RelA - Revision history

Jstuelk at 14:55, 23 April 2015

2015-04-23T14:55:35Z

Jstuelk: /* References */

2015-02-10T17:56:15Z

‎References

Jstuelk: /* Phenotypes of a mutant */

2015-02-10T17:55:28Z

‎Phenotypes of a mutant

Jstuelk: /* Phenotypes of a mutant */

2015-02-10T17:53:15Z

‎Phenotypes of a mutant

Jstuelk: /* Biological materials */

2014-10-23T11:06:09Z

‎Biological materials

Atkovacs: /* Labs working on this gene/protein */

2014-10-23T06:53:28Z

‎Labs working on this gene/protein

Atkovacs: /* Labs working on this gene/protein */

2014-10-23T06:52:54Z

‎Labs working on this gene/protein

Atkovacs: /* Labs working on this gene/protein */

2014-10-23T06:52:39Z

‎Labs working on this gene/protein

Atkovacs: /* References */

2014-10-23T06:47:18Z

‎References

Atkovacs: /* Biological materials */

2014-10-23T06:46:20Z

‎Biological materials

← Older revision		Revision as of 14:55, 23 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' GTP pyrophosphokinase ([[stringent response]]) <br/><br/>~~	+	{{#bank: RelA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''relA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| GTP pyrophosphokinase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| synthesis and degradation of (p)ppGpp, <br/>triggering the [[stringent response]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27600 relA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=relA relA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 84 kDa, 6.964~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2202 bp, 734 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[dtd]]'', ''[[apt]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27600 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:relA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=relA_2820529_2822733_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:relA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27600]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[metabolism of signalling nucleotides]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU27600
−
−	~~===Phenotypes of a mutant ===~~
−	* requirement for valine {{PubMed\|24163341}}
−	* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed\|24163341}}
−	* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant acquires suppressor mutations in ''[[guaA]], [[guaB]], [[gmk]]'' or inactivation of ''[[codY]]'' {{PubMed\|24682323,24163341}}
−	* mutation in ''[[relA]]'' results in increased motility and chaining {{PubMed\|25331430}}
−	* ''[[relA]]'' mutation increases flagellin production via elevated [[SigD]] level {{PubMed\|25331430}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27600&redirect=T BSU27600]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/relA-yrvI.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12665]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)
−
−	* '''Protein family:''' relA/spoT family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−	** N-terminal ppGpp hydrolase domain {{PubMed\|24163341}}
−	** central ppGpp synthetase domain {{PubMed\|24163341}}
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27600&redirect=T BSU27600]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O54408 O54408]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27600]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.6.5 2.7.6.5]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[relA]]-[[dtd]]'' {{PubMed\|9383190}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=relA_2820529_2822733_-1 relA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 244 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 573 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' ''[[relA]]'' mutant {{PubMed\|9383190}} - note that ''[[relA]]'' mutants are prone to suppressor mutations in the ''[[ywaC]]'' or ''[[yjbM]]'' loci {{PubMed\|18670626}}
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]~~
−
−	~~Jue D Wang, University of Wisconsin–Madison [https://www.researchgate.net/profile/Jue_Wang8 ResearchGate-Profile]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>25331430,11948165,9383190,10209741,13129942, 24489751, 19447912 13129942 12372825 12081964 11948165 12419222 23028324 24163341 24682323</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 147: / Line 147: @@
 =References=
-<pubmed>25331430,11948165,9383190,10209741,13129942, 24489751, 19447912 13129942  12372825  12081964  11948165 12419222 23028324 24163341</pubmed>
+<pubmed>25331430,11948165,9383190,10209741,13129942, 24489751, 19447912 13129942  12372825  12081964  11948165 12419222 23028324 24163341 24682323</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 55: / Line 55: @@
 * requirement for valine {{PubMed|24163341}}
 * a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
 * mutation in ''[[relA]]'' results in increased motility and chaining {{PubMed|25331430}}
 * ''[[relA]]'' mutation increases flagellin production via elevated [[SigD]] level {{PubMed|25331430}}

@@ Line 55: / Line 55: @@
 * requirement for valine {{PubMed|24163341}}
 * a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
-* mutation in ''[[relA]]'' results increased motility and chaining {{PubMed|25331430}}
+* mutation in ''[[relA]]'' results in increased motility and chaining {{PubMed|25331430}}
 * ''[[relA]]'' mutation increases flagellin production via elevated [[SigD]] level {{PubMed|25331430}}

← Older revision		Revision as of 11:06, 23 October 2014
Line 126:		Line 126:

	* '''Mutant:''' ''[[relA]]'' mutant {{PubMed\|9383190}} - note that ''[[relA]]'' mutants are prone to suppressor mutations in the ''[[ywaC]]'' or ''[[yjbM]]'' loci {{PubMed\|18670626}}		* '''Mutant:''' ''[[relA]]'' mutant {{PubMed\|9383190}} - note that ''[[relA]]'' mutants are prone to suppressor mutations in the ''[[ywaC]]'' or ''[[yjbM]]'' loci {{PubMed\|18670626}}
−

	* '''Expression vector:'''		* '''Expression vector:'''

@@ Line 142: / Line 142: @@
 [[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
-Jue D Wang, University of Wisconsin–Madison [https://www.researchgate.net/profile/Jue_Wang8]
+Jue D Wang, University of Wisconsin–Madison [https://www.researchgate.net/profile/Jue_Wang8 ResearchGate-Profile]
 =Your additional remarks=

← Older revision		Revision as of 06:52, 23 October 2014
Line 141:		Line 141:

	[[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]		[[Mohamed Marahiel]], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
		+
	Jue D Wang, University of Wisconsin–Madison [https://www.researchgate.net/profile/Jue_Wang8]		Jue D Wang, University of Wisconsin–Madison [https://www.researchgate.net/profile/Jue_Wang8]

← Older revision		Revision as of 06:46, 23 October 2014
Line 125:		Line 125:
	=Biological materials =		=Biological materials =

−	* '''Mutant:''' ''[[relA]]'' mutant {{PubMed\|9383190}} - note that relA ~~single~~ mutants are prone to suppressor mutations in the ''[[ywaC]]'' or ''[[yjbM]]'' ~~genes~~ {{PubMed\|18670626}}	+	* '''Mutant:''' ''[[relA]]'' mutant {{PubMed\|9383190}} - note that ''[[relA]]'' mutants are prone to suppressor mutations in the ''[[ywaC]]'' or ''[[yjbM]]'' loci {{PubMed\|18670626}}