PtsH - Revision history

Jstuelk: /* References */

2015-08-20T12:35:34Z

‎References

Show changes

Fabian Commichau: /* Biological materials */

2014-12-08T14:27:03Z

‎Biological materials

Fabian Commichau: /* Biological materials */

2014-12-08T14:26:11Z

‎Biological materials

Fabian Commichau: /* Biological materials */

2014-12-08T13:21:17Z

‎Biological materials

Jstuelk: /* Extended information on the protein */

2014-06-07T16:05:47Z

‎Extended information on the protein

134.76.70.252 at 14:12, 17 April 2014

2014-04-17T14:12:30Z

134.76.70.252 at 10:01, 17 April 2014

2014-04-17T10:01:40Z

134.76.38.147 at 13:34, 2 April 2014

2014-04-02T13:34:02Z

Jstuelk at 16:16, 5 March 2014

2014-03-05T16:16:37Z

Raphael2215 at 08:35, 7 January 2014

2014-01-07T08:35:23Z

@@ Line 172: / Line 172: @@
 ** pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], available in [[Jörg Stülke]]'s lab
 ** pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Jörg Stülke]]'s lab
-** pGP2431 (N-terminal Strep-tag, purification from ''B. subtilis'', in [[pGP380]]), for [[SPINE]], available in [[Jörg Stülke]]'s lab
+** pGP2431 (N-terminal Strep-tag, expression and purification from ''B. subtilis'', in [[pGP380]]), for [[SPINE]], available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

@@ Line 172: / Line 172: @@
 ** pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], available in [[Jörg Stülke]]'s lab
 ** pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

@@ Line 179: / Line 179: @@
 * '''CFP fusion:''' B. subtilis GP1267 ptsH-cfp ermC- without terminator, available in [[Jörg Stülke]]'s lab
-* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
 * '''Antibody:''' available in [[Jörg Stülke]]'s lab

@@ Line 108: / Line 108: @@
 ** [[PtsH|HPr(Ser-46)-P]]-[[CcpA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12432959 PubMed]
 ** [[PtsH|HPr]]-[[RbsR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16519689 PubMed]
-** [[PtsH|HPr(His)-P]]-[[YesS]] {{PubMed|19651770}}
+** [[PtsH|HPr(His)-P]]-[[RhgR]] {{PubMed|19651770}}
 * '''[[Localization]]:''' cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16395550 PubMed]

@@ Line 154: / Line 154: @@
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 352 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:''' available in [[Jörg Stülke]]'s lab:
 ** MZ303 (cat)

@@ Line 61: / Line 61: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptsGHI.html]
@@ Line 112: / Line 113: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 45: / Line 45: @@
 {{SubtiWiki category|[[phosphotransferase systems]]}},
 {{SubtiWiki category|[[transcription factors and their control]]}},
-{{SubtiWiki category|[[phosphoproteins]]}}
+{{SubtiWiki category|[[phosphoproteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 66: / Line 67: @@
 === Additional information===
 =The protein=
@@ Line 83: / Line 82: @@
 * '''Kinetic information:'''
-* '''Domains:''' HPr Domain (2–88)
+* '''[[Domains]]:''' HPr Domain (2–88)
 * '''Modification:'''
@@ Line 92: / Line 91: @@
 ** ''in vitro'' phosphorylated by [[PrkC]] on Ser-12 {{PubMed|20389117}}
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 151: / Line 150: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 195: / Line 195: @@
 =References=
-<pubmed>16519689,,12850135 17218307 16519689 17142398 12359875 1577686 9162046 11929549 9336834 7803390, 7623661, 2846556, 8169206, 9973552, 15126459, 10048041, 12169607, 9622354, 10217795, 17693724, 18757537 , 9202047 , 7592487, 15369672 , 14527945, 2507315 8580838 19651770 8418852 1303754 1549615 20081037 20389117 20444094 22001508 22722928 23551403</pubmed>
+<pubmed>16519689,,12850135 17218307 16519689 17142398 12359875 1577686 9162046 11929549 9336834 7803390, 7623661, 2846556, 8169206, 9973552, 15126459, 10048041, 12169607, 9622354, 10217795, 17693724, 18757537 , 9202047 , 7592487, 15369672 , 14527945, 2507315 8580838 19651770 8418852 1303754 1549615 20081037 20389117 20444094 22001508 22722928 23551403 15378759</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PtsH PtsH]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ptsH ptsH]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ptsH PtsH]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9,1 kDa, 4.58