PrpC - Revision history

Jstuelk at 14:23, 15 June 2015

2015-06-15T14:23:26Z

Jstuelk at 09:51, 12 July 2014

2014-07-12T09:51:32Z

134.76.70.252 at 10:09, 17 April 2014

2014-04-17T10:09:19Z

134.76.38.147 at 14:10, 2 April 2014

2014-04-02T14:10:16Z

134.76.38.147 at 13:41, 2 April 2014

2014-04-02T13:41:34Z

Jstuelk at 13:23, 7 January 2014

2014-01-07T13:23:51Z

134.76.70.252 at 10:45, 7 January 2014

2014-01-07T10:45:26Z

Jstuelk at 09:28, 1 July 2013

2013-07-01T09:28:55Z

134.76.70.252 at 13:19, 16 May 2013

2013-05-16T13:19:14Z

134.76.70.252 at 10:11, 14 May 2013

2013-05-14T10:11:45Z

← Older revision		Revision as of 14:23, 15 June 2015
Line 1:		Line 1:
−	* '''Description:~~''' [[Protein kinases and phosphatases\|protein phosphatase]] <br/><br/>~~	+	{{#bank: PrpC}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''prpC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yloO ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[Protein kinases and phosphatases\|protein phosphatase]]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| antagonist of [[PrkC]]-dependent phosphorylation~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15760 prpC]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrpC PrpC]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=prpC prpC]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 27 kDa, 4.355~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 762 bp, 254 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yloN]]'', ''[[prkC]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15760 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:prpC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_1650384_1651148_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:prpC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15760]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein modification]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU15760
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~A ''prpC'' mutant is less lytic in late stationary phase. [http://www.ncbi.nlm.nih.gov/sites/entrez/12399479 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13390]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' A phosphoprotein + H<sub>2</sub>O = a protein + phosphate (according to Swiss-Prot)
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Proteins dephosphorylated by PrpC ===~~
−
−	[[CpgA]], [[tufA \| EF-Tu]], [[YezB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed], [[ptsH \| HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed], [[YkwC]] {{PubMed\|24390483}}
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:''' divalent cations such as magnesium or manganese
−
−	* '''Effectors of protein activity:''' inhibited by inorganic phosphate and glycero-2-phosphate [http://www.ncbi.nlm.nih.gov/sites/entrez/16857667 PubMed]
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[YvcK]]-[[PrpC]] {{PubMed\|25012659}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1TXO 1TXO] (from ''Mycobacterium tuberculosis'', 34% identity, 54% similarity) {{PubMed\|15530359}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O34779 O34779]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15760]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.16 3.1.3.16]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[prpC]]-[[prkC]]-[[cpgA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/16025310?ordinalpos=9&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_1650384_1651148_1 prpC] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 459 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' OMG401 (aphA3), available in the [[Stülke]] lab
−	** 1A962 (no resistance), {{PubMed\|12399479}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A962&Search=1A962 BGSC]
−	** 1A964 (no resistance), {{PubMed\|12399479}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A964&Search=1A964 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 21372323 </pubmed>~~
−	~~==Original publications==~~
−	~~<pubmed>19246764,10986276,17693724 , 12399479, 18757537, 15530359 23793375 16025310 24390483 25012659</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 15: / Line 15: @@
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15760 prpC]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=prpC prpC]'''
@@ Line 92: / Line 94: @@
 * '''[[SubtInteract|Interactions]]:'''
 * '''[[Localization]]:'''
@@ Line 115: / Line 118: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_1650384_1651148_1 prpC] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 148: / Line 151: @@
 <pubmed> 21372323 </pubmed>
 ==Original publications==
-<pubmed>19246764,10986276,17693724 , 12399479, 18757537, 15530359 23793375 16025310 24390483</pubmed>
+<pubmed>19246764,10986276,17693724 , 12399479, 18757537, 15530359 23793375 16025310 24390483 25012659</pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 10:09, 17 April 2014
Line 122:		Line 122:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 459 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 94: / Line 95: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1TXO 1TXO] (from ''Mycobacterium tuberculosis'', 34% identity, 54% similarity) {{PubMed|15530359}}

@@ Line 62: / Line 62: @@
 === Additional information===
 =The protein=
@@ Line 78: / Line 75: @@
 === Proteins dephosphorylated by PrpC ===
-[[CpgA]],  [[tufA | EF-Tu]], [[YezB]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed], [[ptsH | HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537  PubMed]
+[[CpgA]],  [[tufA | EF-Tu]], [[YezB]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed], [[ptsH | HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537  PubMed], [[YkwC]] {{PubMed|24390483}}
 === Extended information on the protein ===
@@ Line 84: / Line 81: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):''' divalent cations such as magnesium or manganese
+* '''[[Cofactors]]:''' divalent cations such as magnesium or manganese
 * '''Effectors of protein activity:''' inhibited by inorganic phosphate and glycero-2-phosphate [http://www.ncbi.nlm.nih.gov/sites/entrez/16857667 PubMed]
@@ Line 146: / Line 143: @@
 <pubmed> 21372323 </pubmed>
 ==Original publications==
-<pubmed>19246764,10986276,17693724 , 12399479, 18757537, 15530359 23793375 16025310</pubmed>
+<pubmed>19246764,10986276,17693724 , 12399479, 18757537, 15530359 23793375 16025310 24390483</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15760 prpC]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=prpC prpC]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 4.355

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 150: / Line 146: @@
 <pubmed> 21372323 </pubmed>
 ==Original publications==
-'''Additional publications:''' {{PubMed|16025310}}
+<pubmed>19246764,10986276,17693724 , 12399479, 18757537, 15530359 23793375 16025310</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_1650384_1651148_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:prpC_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_1650384_1651148_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:prpC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15760]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yloN]]'', ''[[prkC]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15760 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15760 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:prpC_context.gif]]