PbpA - Revision history

134.76.38.147 at 14:14, 2 April 2014

2014-04-02T14:14:00Z

Jstuelk at 09:24, 5 March 2014

2014-03-05T09:24:12Z

Jstuelk at 09:09, 5 March 2014

2014-03-05T09:09:36Z

134.76.70.252 at 08:52, 12 November 2013

2013-11-12T08:52:26Z

Jstuelk at 06:47, 4 June 2013

2013-06-04T06:47:02Z

134.76.70.252 at 13:55, 16 May 2013

2013-05-16T13:55:58Z

134.76.70.252 at 10:44, 14 May 2013

2013-05-14T10:44:08Z

134.76.70.252 at 13:19, 13 May 2013

2013-05-13T13:19:05Z

134.76.70.252 at 13:51, 7 August 2012

2012-08-07T13:51:16Z

134.76.70.252 at 08:33, 20 April 2012

2012-04-20T08:33:35Z

← Older revision		Revision as of 14:14, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' class B [[penicillin-binding protein]] PBP 2A, required for [[cell wall synthesis]] during cell elongation <br/><br/>~~	+	{{#bank: PbpA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''pbpA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yqgF ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[penicillin-binding protein]] PBP 2A~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| formation of a rod-shaped peptidoglycan cell wall, spore outgrowth~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25000 pbpA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PbpA PbpA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 79 kDa, 9.571~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2148 bp, 716 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[pstS]]'', ''[[yqgE]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25000 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:pbpA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpA_2581771_2583921_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:pbpA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25000]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell wall synthesis]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU25000
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11678]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** required for [[cell wall synthesis]] during cell elongation {{PubMed\|12896990}}
−	** driver of [[MreB]] dynamics {{PubMed\|21636744,21636745}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** part of the [[elongasome]] {{PubMed\|21636744,21636745}}
−	** folding requires [[PrsA]] {{PubMed\|20487272}}
−
−	* '''[[Localization]]:'''
−	** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
−	** localization depends on the availability of peptidoglyan precursors (lipid II) {{PubMed\|23895585}}
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P54488 P54488]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25000]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[pbpA]]'' {{PubMed\|21815947}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpA_2581771_2583921_-1 pbpA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** the mRNA is substantially stabilized upon depletion of [[Rny\|RNase Y]] {{PubMed\|21815947}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>9851991,9139922,12896990 ,18957862, 20487272 21636744,21636745 21815947 23895585 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 1: / Line 1: @@
-* '''Description:''' class B [[penicillin-binding protein]] PBP 2A <br/><br/>
+* '''Description:''' class B [[penicillin-binding protein]] PBP 2A, required for [[cell wall synthesis]] during cell elongation <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 67: / Line 67: @@
 * '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:'''
@@ Line 85: / Line 87: @@
 * '''[[SubtInteract|Interactions]]:'''
-** part of the [[cell wall biosynthetic complex]] {{PubMed|21636744,21636745}}
+** part of the [[elongasome]] {{PubMed|21636744,21636745}}
 ** folding requires [[PrsA]] {{PubMed|20487272}}
-* '''[[Localization]]:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
+* '''[[Localization]]:'''
 === Database entries ===

@@ Line 1: / Line 1: @@
-* '''Description:''' [[penicillin-binding protein]] PBP 2A <br/><br/>
+* '''Description:''' class B [[penicillin-binding protein]] PBP 2A <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 76: / Line 76: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 138: / Line 138: @@
 =References=
-<pubmed>9851991,9139922,12896990 ,18957862, 20487272 21636744,21636745 21815947 </pubmed>
+<pubmed>9851991,9139922,12896990 ,18957862, 20487272 21636744,21636745 21815947 23895585 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25000 pbpA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PbpA PbpA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PbpA PbpA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 9.571

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 112: / Line 108: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpA_2581771_2583921_-1 pbpA] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 142: / Line 138: @@
 =References=
- <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J''  </big>
+<pubmed>9851991,9139922,12896990 ,18957862, 20487272 21636744,21636745 21815947 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpA_2581771_2583921_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:pbpA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pbpA_2581771_2583921_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:pbpA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25000]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pstS]]'', ''[[yqgE]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25000 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25000 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:pbpA_context.gif]]

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || formation of a rod-shaped peptidoglycan cell wall, spore outgrowth
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PbpA PbpA]