PHT253 - Revision history

Bzhu at 11:35, 28 August 2017

2017-08-28T11:35:42Z

Bzhu at 09:24, 28 August 2017

2017-08-28T09:24:32Z

Bzhu at 13:41, 21 August 2017

2017-08-21T13:41:29Z

Bzhu at 13:41, 21 August 2017

2017-08-21T13:41:21Z

Bzhu: Created page with "{{ContentDislamer}} 600px 230px The pHT253 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular p..."

2017-08-21T13:40:30Z

Created page with "{{ContentDislamer}} 600px 230px The pHT253 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular p..."

New page

{{ContentDislamer}}

[[File:PHT253.PNG|600px]]
[[File:Legend.png|230px]]

The pHT253 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant N-terminal His tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.
The expression is controlled by the strong P''grac100'' promoter, consisting of the ''groeESL'' promoter of ''B. subtilis'' with improved regulatory elements fused to the ''lac'' operator allowing their induction by IPTG. Nucleotides were optimized at the conserved regions of the ''groESL'' promoter including the UP element, the −35 and the −15 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein of ''B. subtilis'' (Phan ''et al''., 2011). pHT253 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.

=== Features ===
* Strong promoter with improved regulatory elements
* Enhanced amount of produced recombinant proteins
* IPTG inducible gene expression
* N-terminal 8xHis-tag
* ''E. coli / B. subtilis'' shuttle vector

The pHT253 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]

== References ==
<pubmed>17624574, 16005967, 16125412</pubmed>

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	== References ==		== References ==
−	<pubmed>~~17624574~~, ~~16005967~~, ~~16125412~~</pubmed>	+	<pubmed>16125412, 22100269, 25990516</pubmed>

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 * ''E. coli / B. subtilis'' shuttle vector
-The pHT253 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]
+The pHT253 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]]
 == References ==
 <pubmed>16125412, 22100269, 25990516</pubmed>

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 [[File:PHT253.PNG|600px]]
-[[File:Legend.png|230px]]
+[[File:PHT253-Legende.jpg|300px]]
 The pHT253 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant N-terminal His tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.