MtlA - Revision history

Jstuelk: /* Original publications */

2014-08-01T14:36:11Z

‎Original publications

134.76.38.147 at 12:59, 2 April 2014

2014-04-02T12:59:55Z

134.76.70.252 at 10:09, 7 January 2014

2014-01-07T10:09:57Z

134.76.70.252 at 15:12, 11 November 2013

2013-11-11T15:12:41Z

134.76.70.252 at 12:33, 16 May 2013

2013-05-16T12:33:59Z

134.76.70.252 at 09:29, 14 May 2013

2013-05-14T09:29:32Z

134.76.70.252 at 12:09, 13 May 2013

2013-05-13T12:09:21Z

Jstuelk at 08:59, 21 January 2013

2013-01-21T08:59:46Z

Jstuelk at 16:03, 3 January 2013

2013-01-03T16:03:52Z

Raphael2215 at 15:48, 3 January 2013

2013-01-03T15:48:13Z

← Older revision		Revision as of 14:36, 1 August 2014
Line 1:		Line 1:
−	* '''Description:~~''' [[trigger enzymes\|trigger enzyme]]: mannitol-specific [[PTS\|phosphotransferase system]], EIICB of the [[PTS]]<br/><br/>~~	+	{{#bank: MtlA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mtlA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[trigger enzymes\|trigger enzyme]]: mannitol-specific [[PTS\|phosphotransferase system]], EIICB~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| mannitol uptake and phosphorylation, control of [[MtlR]] activity~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03981 mtlA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MtlA MtlA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mtlA mtlA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 50 kDa, 8.814~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1434 bp, 478 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ycnL]]'', ''[[mtlF]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03981 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03981 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03981 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mtlA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mtlA_449724_451160_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:mtlA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03981]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[phosphotransferase systems]]}},~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}},~~
−	~~{{SubtiWiki category\|[[transcription factors and their control]]}},~~
−	~~{{SubtiWiki category\|[[trigger enzyme]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[MtlR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU03981
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03981&redirect=T BSU03981]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mtlAD.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11215]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
−
−	* '''Protein family:''' [[PTS]] permease, fructose/ mannitol permease (Fru) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:''' phosphorylation on Ser-559 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[MtlA]]-[[MtlR]] {{PubMed\|20444094}} (the non-phosphorylated IIB domain of [[MtlA]] interacts with the two C-terminal EIIB (Gat)-like and EIIA (Mtl)-like domains of [[MtlR]]) {{PubMed\|23279188}}
−
−	* '''[[Localization]]:'''
−	** cell membrane (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03981&redirect=T BSU03981]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A3A 1A3A] (from ''Escherichia coli k12 mutant'', 42% identity, 66% similarity) {{PubMed\|9551558}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P42956 P42956]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU03980]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[mtlA]]-[[mtlF]]-[[mtlD]]'' {{PubMed\|12897001}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mtlA_449724_451160_1 mtlA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|22014119}}
−
−	* '''Regulation:'''
−	** induced by mannitol ([[MtlR]]) {{PubMed\|20444094}}
−	** repressed by glucose ([[MtlR]]) {{PubMed\|20444094}}
−
−	* '''Regulatory mechanism:'''
−	** [[MtlR]]: transcription activation {{PubMed\|20444094}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 23318733 </pubmed>~~
−	~~==Original publications==~~
−	~~'''Additional publications:''' {{PubMed\|23279188,22014119}}~~
−	~~<pubmed>12897001, 20444094, 10627040 17218307</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 63: / Line 63: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mtlAD.html]
@@ Line 102: / Line 103: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A3A 1A3A] (from ''Escherichia coli k12 mutant'', 42% identity, 66% similarity)	{{PubMed|9551558}}

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MtlA MtlA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mtlA mtlA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 8.814

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03981 mtlA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MtlA MtlA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MtlA MtlA]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''

@@ Line 33: / Line 33: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mtlA_449724_451160_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mtlA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mtlA_449724_451160_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mtlA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03981]]
 |-
 |}

@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycnL]]'', ''[[mtlF]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03981 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03981 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03981 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03981 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03981 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03981 DNA_with_flanks]
 |-
 |-

@@ Line 119: / Line 119: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mtlA_449724_451160_1 mtlA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|22014119}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|22014119}}
 * '''Regulation:'''
@@ Line 149: / Line 149: @@
 =References=
 '''Additional publications:''' {{PubMed|23279188,22014119}}
 <pubmed>12897001, 20444094, 10627040 17218307</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 38: / Line 38: @@
 __TOC__
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[phosphotransferase systems]]}},