MotA - Revision history

Jstuelk: /* Biological materials */

2015-07-03T14:47:08Z

‎Biological materials

Atkovacs at 13:14, 2 July 2015

2015-07-02T13:14:41Z

Jstuelk at 08:17, 5 May 2014

2014-05-05T08:17:27Z

134.76.70.252 at 09:53, 17 April 2014

2014-04-17T09:53:11Z

134.76.38.147 at 13:33, 2 April 2014

2014-04-02T13:33:07Z

Jstuelk: /* Phenotypes of a mutant */

2013-12-04T20:16:07Z

‎Phenotypes of a mutant

Jstuelk at 20:09, 4 December 2013

2013-12-04T20:09:55Z

134.76.70.252 at 16:07, 11 November 2013

2013-11-11T16:07:08Z

Jstuelk at 10:31, 10 August 2013

2013-08-10T10:31:38Z

Jstuelk: /* References */

2013-06-19T10:19:31Z

‎References

← Older revision		Revision as of 14:47, 3 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' H+-coupled [[~~MotA~~]]-[[MotB]] flagellar stator <br/><br/>~~	+	{{#bank: MotA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''motA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''mot ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| motility protein (flagellar motor rotation)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[motility and chemotaxis]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13690 motA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MotA MotA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 29 kDa, 5.01~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 810 bp, 270 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[motB]]'', ''[[clpE]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13690 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:motA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motA_1434433_1435245_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:motA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13690]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[motility and chemotaxis]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigD regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU13690
−
−	~~===Phenotypes of a mutant ===~~
−	* loss of swimming and swarming motility {{PubMed\|24296669}}
−	* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed\|24296669}}
−	* not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed\|26122431}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13690&redirect=T BSU13690]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/motAB.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10688]
−
−	~~=== Additional information===~~
−	* A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed\|21821766}}
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' motA family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[MotP]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−	** the interaction with [[DgrA]] inhibits [[MotA]] activity {{PubMed\|22821967}}
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[MotA]]-[[MotB]]
−	** [[DgrA]]-[[MotA]] {{PubMed\|22821967}}
−
−	* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13690&redirect=T BSU13690]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P28611 P28611]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13690]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[motA]]-[[motB]]'' {{PubMed\|6313226}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motA_1434433_1435245_-1 motA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigD]] {{PubMed\|6313226}}
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 100 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 613 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A631 ( ''motA''::''erm''), {{PubMed\|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC]
−	** 1A923 ( ''motA''::''erm''), {{PubMed\|12813063}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC]
−	** DS7498 (marker-less in NCIB3610) {{PubMed\|24296669}}
−	** TB193 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed\|26122431}}
−	** TB210 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed\|26122431}}
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed> 26122431, 6313226 21821766 22821967 24296669 24771657 </pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 55: / Line 55: @@
 * loss of swimming and swarming motility {{PubMed|24296669}}
 * mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
 === Database entries ===
@@ Line 128: / Line 129: @@
 ** 1A631 ( ''motA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC]
 ** 1A923 ( ''motA''::''erm''), {{PubMed|12813063}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC]
 * '''Expression vector:'''
@@ Line 144: / Line 148: @@
 =References=
-<pubmed> 6313226 21821766 22821967 24296669 24771657 </pubmed>
+<pubmed> 26122431, 6313226 21821766 22821967 24296669 24771657 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 74: / Line 74: @@
 * '''Protein family:''' motA family (according to Swiss-Prot)
-* '''Paralogous protein(s):'''
+* '''Paralogous protein(s):''' [[MotP]]
 === Extended information on the protein ===
@@ Line 144: / Line 144: @@
 =References=
-<pubmed> 6313226 21821766 22821967 24296669 </pubmed>
+<pubmed> 6313226 21821766 22821967 24296669 24771657 </pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 09:53, 17 April 2014
Line 120:		Line 120:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 100 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 613 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 57: / Line 57: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/motAB.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 53: / Line 53: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 77: / Line 79: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
 ** the interaction with [[DgrA]] inhibits [[MotA]] activity {{PubMed|22821967}}
 * '''[[SubtInteract|Interactions]]:'''
 ** [[MotA]]-[[MotB]]
@@ Line 137: / Line 140: @@
 =References=
-<pubmed> 6313226 21821766 22821967</pubmed>
+<pubmed> 6313226 21821766 22821967 24296669 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13690 motA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MotA MotA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MotA MotA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.01

@@ Line 84: / Line 84: @@
 * '''Effectors of protein activity:'''
-** the interaction with [[YpfA]] inhibits [[MotA]] activity {{PubMed|22821967}}
+** the interaction with [[DgrA]] inhibits [[MotA]] activity {{PubMed|22821967}}
 * '''[[SubtInteract|Interactions]]:'''
 ** [[MotA]]-[[MotB]]
-** [[YpfA]]-[[MotA]] {{PubMed|22821967}}
+** [[DgrA]]-[[MotA]] {{PubMed|22821967}}
 * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
@@ Line 109: / Line 109: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=motA_1434433_1435245_-1 motA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigD]] {{PubMed|6313226}}
+* '''[[Sigma factor]]:''' [[SigD]] {{PubMed|6313226}}
 * '''Regulation:'''