MetE - Revision history

Jstuelk: /* References */

2015-04-10T11:02:21Z

‎References

134.76.70.252 at 14:10, 17 April 2014

2014-04-17T14:10:06Z

134.76.70.252 at 09:23, 17 April 2014

2014-04-17T09:23:58Z

134.76.38.147 at 13:31, 2 April 2014

2014-04-02T13:31:19Z

Jstuelk at 12:54, 5 March 2014

2014-03-05T12:54:03Z

134.76.70.252 at 10:36, 7 January 2014

2014-01-07T10:36:31Z

Jstuelk at 14:07, 22 December 2013

2013-12-22T14:07:35Z

Jstuelk: /* Extended information on the protein */

2013-12-13T19:19:22Z

‎Extended information on the protein

Jstuelk at 19:13, 13 December 2013

2013-12-13T19:13:19Z

Jstuelk: /* Extended information on the protein */

2013-12-13T18:53:03Z

‎Extended information on the protein

← Older revision		Revision as of 11:02, 10 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' methionine synthase <br/><br/>~~	+	{{#bank: MetE}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''metE''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''metC ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| methionine synthase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| biosynthesis of methionine~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13180 metE]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MetE MetE]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=metE metE]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 86 kDa, 4.839~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2286 bp, 762 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[guaD]]'', ''[[ispA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13180 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:metE_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=metE_1383320_1385608_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:metE_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13180]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis/ acquisition of amino acids]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}},~~
−	~~[[most abundant proteins]]~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[S-box]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU13180
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13180&redirect=T BSU13180]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12616]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine (according to Swiss-Prot)
−
−	* '''Protein family:''' vitamin-B12 independent methionine synthase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−	**phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed], S-cysteinylation after diamide stress (C719) [http://www.ncbi.nlm.nih.gov/sites/entrez/17611193 PubMed]
−	** Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species {{PubMed\|21749987}} {{PubMed\|22938038}}
−	** MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species {{PubMed\|21749987}} {{PubMed\|22938038}}
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[BrxA]]-[[MetE]], to de-bacillithiolate S-bacillithiolated [[MetE]] {{PubMed\|24313874}}
−	** [[BrxB]]-[[MetE]], to de-bacillithiolate S-bacillithiolated [[MetE]] {{PubMed\|24313874}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13180&redirect=T BSU13180]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1T7L 1T7L] (from ''Thermotoga maritima'', 44% identity, 61% similarity) {{PubMed\|15630480}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P80877 P80877]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13180]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.1.1.14 2.1.1.14]
−
−	~~=== Additional information===~~
−
−	~~:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]~~
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''metE''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=metE_1383320_1385608_-1 metE] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
−	** repressed by methionine (about 1000-fold)([[S-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed]
−
−	* '''Regulatory mechanism:''' [[S-box]]: transcription termination/ antitermination, the [[S-box]] [[riboswitch]] binds S-adenosylmethionine resulting in termination [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed]
−
−	* '''Additional information:'''
−	** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
−	** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 10685 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 1682 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9230 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 4809 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3703 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−	** 1A607 ( ''metE''::''erm''), {{PubMed\|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A607&Search=1A607 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>21749987 22938038,21749987,19258532,16194229,10094622,17611193,18039762 ,17726680 12107147 24313874 24163341 15378759</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 131: / Line 131: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 10685 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1682 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''
 ** 1A607 ( ''metE''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A607&Search=1A607 BGSC]

@@ Line 129: / Line 129: @@
 ** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 98: / Line 99: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1T7L 1T7L] (from ''Thermotoga maritima'', 44% identity, 61% similarity) {{PubMed|15630480}}

@@ Line 43: / Line 43: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
-{{SubtiWiki category|[[phosphoproteins]]}}
+{{SubtiWiki category|[[phosphoproteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 123: / Line 124: @@
 * '''Regulatory mechanism:''' [[S-box]]: transcription termination/ antitermination, the [[S-box]] [[riboswitch]] binds S-adenosylmethionine resulting in termination [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed]
-* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
+* '''Additional information:'''
 =Biological materials =
@@ Line 145: / Line 148: @@
 =References=
-<pubmed>21749987 22938038,21749987,19258532,16194229,10094622,17611193,18039762 ,17726680 12107147 24313874 24163341</pubmed>
+<pubmed>21749987 22938038,21749987,19258532,16194229,10094622,17611193,18039762 ,17726680 12107147 24313874 24163341 15378759</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MetE MetE]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=metE metE]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 86 kDa, 4.839

@@ Line 91: / Line 91: @@
 * '''[[SubtInteract|Interactions]]:'''
-** [[BrxA]]-[[MetE]] {{PubMed|24313874}}
+** [[BrxA]]-[[MetE]], to de-bacillithiolate S-bacillithiolated [[MetE]] {{PubMed|24313874}}
-** [[BrxB]]-[[MetE]] {{PubMed|24313874}}
+** [[BrxB]]-[[MetE]], to de-bacillithiolate S-bacillithiolated [[MetE]] {{PubMed|24313874}}
 * '''[[Localization]]:'''

@@ Line 83: / Line 83: @@
 ** Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species {{PubMed|21749987}} {{PubMed|22938038}}
 ** MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species {{PubMed|21749987}} {{PubMed|22938038}}
 * '''[[Cofactors]]:'''