MecA - Revision history

134.76.70.252 at 14:09, 17 April 2014

2014-04-17T14:09:57Z

134.76.70.252 at 10:00, 17 April 2014

2014-04-17T10:00:00Z

134.76.38.147 at 13:25, 2 April 2014

2014-04-02T13:25:14Z

134.76.70.252 at 16:00, 11 November 2013

2013-11-11T16:00:40Z

Jstuelk at 15:52, 14 June 2013

2013-06-14T15:52:25Z

134.76.70.252 at 12:59, 16 May 2013

2013-05-16T12:59:10Z

134.76.70.252 at 09:55, 14 May 2013

2013-05-14T09:55:04Z

134.76.70.252 at 12:32, 13 May 2013

2013-05-13T12:32:59Z

Jstuelk at 11:39, 14 March 2013

2013-03-14T11:39:50Z

Jstuelk at 12:45, 5 February 2013

2013-02-05T12:45:05Z

← Older revision		Revision as of 14:09, 17 April 2014
Line 1:		Line 1:
−	* '''Description:''' buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets [[ComK]] and [[ComS]] to [[ClpC]]-[[ClpP]] degradation machine (in log Phase), inhibits the transcriptional activity of [[Spo0A]]∼P by direct interaction <br/><br/>	+	{{#bank: MecA}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''mecA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| adaptor protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| control of [[ComK]] degradation, regulation of competence~~
−	\|-
−	~~\|colspan="2" style="background:~~#~~FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http~~:~~//subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11520 mecA]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MecA ~~MecA]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 25 kDa, 4.209~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 654 bp, 218 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yjbE]]'', ''[[coiA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11520 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:mecA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mecA_1229068_1229724_1 Expression at a glance]'''   {{PubMed\|22383849~~}~~}<br/>[[Image:mecA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11520]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[genetic competence]]}},~~
−	~~{{SubtiWiki category\|[[proteolysis]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU11520
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11520&redirect=T BSU11520]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mecA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10680]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' mecA family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[YpbH]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−	** N-terminal domain (1 ... 120): recruitment of protein substrates {{PubMed\|19801546}}
−	** C-terminal domain (121 ... 218): facilitates assembly of the [[ClpC]]-[[ClpP]] oligomer and acts as a degradation tag {{PubMed\|19801546}}
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[MecA]]-[[ComK]] {{PubMed\|10447896}}
−	** [[MecA]]-[[ComS]] {{PubMed\|10447896}}
−	** [[ClpC]]-[[MecA]] {{PubMed\|10447896}}
−	** [[MecA]]-[[Spo0A]] {{PubMed\|21435029}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11520&redirect=T BSU11520]
−
−	* '''Structure:'''
−	** [http://www.proteopedia.org/wiki/index.php/3jtp 3JTP] (C-terminal domain) {{PubMed\|19801546}}
−	** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3PXG 3PXG], [http://www.rcsb.org/pdb/explore/explore.do?pdbId= 3J3U 3J3U] (the [[MecA]]-[[ClpC]] complex) {{PubMed\|23595989}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P37958 P37958]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11520]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[mecA]]'' {{PubMed\|8412687}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mecA_1229068_1229724_1 mecA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|8412687}}
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 53 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' GP813 (spc), GP814 (aphA3) both available in the [[Stülke]] lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews ==~~
−	~~<pubmed> 23375660 23479438,19609260</pubmed>~~
−	~~==Original Publications==~~
−	<pubmed>11914365,9000055,17560370,19361434, 9890793,2113920,8817496, , 8412687,11918817, 10447896, 12028382, 8016067, 10361283, 11703662 9535081 19767395 19801546 8016066 19202088 8083167 21368759 23595989 16525504,12598648,21435029</pubmed>
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 10:00, 17 April 2014
Line 122:		Line 122:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 53 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 54: / Line 54: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mecA.html]
@@ Line 94: / Line 95: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11520 mecA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MecA MecA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MecA MecA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 4.209

@@ Line 1: / Line 1: @@
-* '''Description:''' buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets [[ComK]] to [[ClpC]]-[[ClpP]] degradation machine (in log Phase), inhibits the transcriptional activity of [[Spo0A]]∼P by direct interaction <br/><br/>
+* '''Description:''' buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets [[ComK]] and [[ComS]] to [[ClpC]]-[[ClpP]] degradation machine (in log Phase), inhibits the transcriptional activity of [[Spo0A]]∼P by direct interaction <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-
@@ Line 97: / Line 97: @@
 * '''Structure:'''
 ** [http://www.proteopedia.org/wiki/index.php/3jtp 3JTP] (C-terminal domain) {{PubMed|19801546}}
-** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3PXG 3PXG] (the [[MecA]]-[[ClpC]] complex) {{PubMed|21368759}}
+** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3PXG 3PXG], [http://www.rcsb.org/pdb/explore/explore.do?pdbId= 3J3U 3J3U] (the [[MecA]]-[[ClpC]] complex) {{PubMed|23595989}}
 * '''UniProt:''' [http://www.uniprot.org/uniprot/P37958 P37958]
@@ Line 141: / Line 141: @@
 =References=
 ==Reviews ==
-'''Additional reviews:''' {{PubMed|23479438,19609260}}
+<pubmed> 23375660 23479438,19609260</pubmed>
 ==Original Publications==
-'''Additonal publications:''' {{PubMed|16525504,12598648,21435029}}
+<pubmed>11914365,9000055,17560370,19361434, 9890793,2113920,8817496, , 8412687,11918817, 10447896, 12028382, 8016067, 10361283, 11703662  9535081 19767395 19801546 8016066 19202088 8083167 21368759 23595989 16525504,12598648,21435029</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 28: / Line 28: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mecA_1229068_1229724_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mecA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mecA_1229068_1229724_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mecA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11520]]
 |-
 |}

@@ Line 23: / Line 23: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbE]]'', ''[[coiA]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11520 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11520 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:mecA_context.gif]]

@@ Line 36: / Line 36: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 117: / Line 113: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mecA_1229068_1229724_1 mecA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|8412687}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8412687}}
 * '''Regulation:'''
@@ Line 144: / Line 140: @@
 =References=
-==Reviews: {{PubMed|19609260}}==
+==Reviews ==
 ==Original Publications==
 '''Additonal publications:''' {{PubMed|16525504,12598648,21435029}}
 <pubmed>11914365,9000055,17560370,19361434, 9890793,2113920,8817496, , 8412687,11918817, 10447896, 12028382, 8016067, 10361283, 11703662  9535081 19767395 19801546 8016066 19202088 8083167 21368759</pubmed>
 [[Category:Protein-coding genes]]