KtrA - Revision history

Jstuelk: /* Biological materials */

2015-07-20T08:34:37Z

‎Biological materials

Jstuelk: /* Original publications */

2015-05-14T13:20:00Z

‎Original publications

Jstuelk: /* Original publications */

2015-05-11T16:56:39Z

‎Original publications

Jstuelk: /* References */

2015-04-17T16:06:21Z

‎References

Jstuelk at 08:18, 1 December 2014

2014-12-01T08:18:33Z

134.76.38.147 at 14:36, 2 April 2014

2014-04-02T14:36:33Z

Jkampf: /* Biological materials */

2014-03-14T09:24:22Z

‎Biological materials

Jkampf: /* Biological materials */

2014-03-14T09:20:21Z

‎Biological materials

Jstuelk: /* Expression and regulation */

2014-02-19T15:51:25Z

‎Expression and regulation

134.76.70.252 at 11:32, 7 January 2014

2014-01-07T11:32:37Z

← Older revision		Revision as of 08:34, 20 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' high affinity potassium transporter [[~~KtrA~~]]-[[KtrB]], peripheric membrane component (proton symport) <br/><br/>~~	+	{{#bank: KtrA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ktrA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yuaA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| high affinity potassium transporter [[KtrA]]-[[KtrB]], <br/>peripheric membrane component (proton symport)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| potassium uptake~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31090 ktrA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KtrA KtrA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ktrA ktrA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 24 kDa, 5.981~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 666 bp, 222 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[bslA]]'', ''[[ktrB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31090 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yuaA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrA_3188414_3189082_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ktrA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31090]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[transporters/ other]]}},~~
−	~~{{SubtiWiki category\|[[metal ion homeostasis (K, Na, Ca, Mg)]]}},~~
−	~~{{SubtiWiki category\|[[coping with hyper-osmotic stress]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[YdaO riboswitch]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU31090
−
−	~~===Phenotypes of a mutant ===~~
−	* The ''[[ktrA]]-[[ktrB]]'' mutant of ''B. subtilis'' NCIB3610 is reduced in sliding (dendritic spreading) {{PubMed\|16321950}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31090&redirect=T BSU31090]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13943]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[KtrC]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−	** contains a [[RCK_N domain]] at the N-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+N-terminal+domain*%22])
−	** contains a [[RCK_C domain]] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+C-terminal+domain*%22])
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[KtrA]]-[[KtrB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed], composed of an octameric [[KtrA]] ring and two [[KtrB]] dimers {{PubMed\|23598340}}
−
−	* '''[[Localization]]:''' peripheral membrane protein {{PubMed\|12562800}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU31090&redirect=T BSU31090]
−
−	* '''Structure:'''
−	** [http://www.rcsb.org/pdb/explore.do?structureId=1LSU 1LSU] (complex with NADH)
−	** [http://www.proteopedia.org/wiki/index.php/2hmw 2HMW] ( complex with ATP)
−	** [http://www.pdb.org/pdb/explore/explore.do?structureId=4J7C 4J7C] (the [[KtrA]]-[[KtrB]] complex) {{PubMed\|23598340}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O32080 O32080]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31090]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[ktrA]]-[[ktrB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrA_3188414_3189082_1 ktrA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−	** expression is controlled via termination antitermination by the [[ydaO riboswitch]], expression is switched off upon binding of c-di-AMP {{PubMed\|24141192,20511502}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A954 ( ''ktrA''::''kan''), {{PubMed\|12562800}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
−	** GHB1 (D(''[[ktrA]]-[[ktrB]]'')::''aphA3''), available in [[Erhard Bremer]]'s lab
−	** GP92 (D(''[[ktrA]]-[[ktrB]]'')::''aphA3''), available in [[Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−	**pGP2594: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Erhard Bremer]], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>25869574 </pubmed>~~
−	~~==Original publications==~~
−	~~<pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 16990138 24141192 25957408 16990138</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 157: / Line 157: @@
 <pubmed>25869574 </pubmed>
 ==Original publications==
-<pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 16990138 24141192 25957408</pubmed>
+<pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 16990138 24141192 25957408 16990138</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 157: / Line 157: @@
 <pubmed>25869574 </pubmed>
 ==Original publications==
-<pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 16990138 24141192</pubmed>
+<pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 16990138 24141192 25957408</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 154: / Line 154: @@
 =References=
+==Reviews==
 <pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 16990138 24141192</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 85: / Line 85: @@
 * '''[[Domains]]:'''
 ** contains a [[RCK_N domain]] at the N-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+N-terminal+domain*%22])
-** contains a c-di-AMP-binding [[RCK_C domain]] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+C-terminal+domain*%22]) {{PubMed|23671116}}
+** contains a [[RCK_C domain]] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+C-terminal+domain*%22])
 * '''Modification:'''
@@ Line 92: / Line 92: @@
 * '''Effectors of protein activity:'''
 * '''[[SubtInteract|Interactions]]:'''
@@ Line 156: / Line 155: @@
 =References=
-<pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 23671116 16990138 24141192</pubmed>
+<pubmed>12562800,15096624,16321950, 20511502, 23086297 23598340 16990138 24141192</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 60: / Line 60: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 99: / Line 100: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 136: / Line 136: @@
 * '''Expression vector:'''
-**pGP2594: (expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
+**pGP2594: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

← Older revision		Revision as of 09:20, 14 March 2014
Line 136:		Line 136:

	* '''Expression vector:'''		* '''Expression vector:'''
		+	**pGP2594: (expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab

	* '''lacZ fusion:'''		* '''lacZ fusion:'''

@@ Line 124: / Line 124: @@
 * '''Regulatory mechanism:'''
-** expression is controlled via termination antitermination by the [[ydaO riboswitch]], expression is switched off upon binding od c-di-AMP {{PubMed|24141192,20511502}}
+** expression is controlled via termination antitermination by the [[ydaO riboswitch]], expression is switched off upon binding of c-di-AMP {{PubMed|24141192,20511502}}
 * '''Additional information:'''

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=KtrA KtrA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis], [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ktrA ktrA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.981