IlvC - Revision history

Jstuelk: /* References */

2015-07-30T13:00:36Z

‎References

Jstuelk: /* References */

2015-07-30T09:17:08Z

‎References

Jstuelk: /* References */

2014-09-19T12:36:42Z

‎References

134.76.70.252 at 14:09, 17 April 2014

2014-04-17T14:09:07Z

134.76.70.252 at 09:23, 17 April 2014

2014-04-17T09:23:52Z

134.76.38.147 at 14:25, 2 April 2014

2014-04-02T14:25:51Z

Jstuelk at 13:22, 5 March 2014

2014-03-05T13:22:49Z

134.76.70.252 at 11:23, 7 January 2014

2014-01-07T11:23:20Z

Jstuelk at 12:20, 22 December 2013

2013-12-22T12:20:15Z

134.76.70.252 at 14:11, 16 May 2013

2013-05-16T14:11:44Z

← Older revision		Revision as of 13:00, 30 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' ketol-acid reductoisomerase (2,3-dihydroxy-3-methylbutanoate, 2-acetolactate)<br/><br/>~~	+	{{#bank: IlvC}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ilvC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| ketol-acid reductoisomerase <br/>(2,3-dihydroxy-3-methylbutanoate, 2-acetolactate)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| biosynthesis of branched-chain amino acids~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28290 ilvC]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ilvC ilvC]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 37 kDa, 5.37~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1026 bp, 342 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[leuA]]'', ''[[ilvH]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28290 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28290 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28290 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:ilvC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvC_2893681_2894709_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ilvC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28290]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis/ acquisition of amino acids]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}},~~
−	~~[[most abundant proteins]]~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[CodY regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[T-box]]}},~~
−	~~{{SubtiWiki regulon\|[[TnrA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU28290
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28290&redirect=T BSU28290]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ilvBHC-leuABCD.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10672]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' (R)-2,3-dihydroxy-3-methylbutanoate + NADP<sup>+</sup> = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH (according to Swiss-Prot)
−
−	* '''Protein family:''' ketol-acid reductoisomerase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−	** phosphorylated on Arg-298 {{PubMed\|22517742}}
−	** phosphorylated on Ser-338 {{PubMed\|20509597}}
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28290&redirect=T BSU28290]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NP3 1NP3] (from ''Escherichia coli'', 53% identity, 71% similarity) {{PubMed\|12691757}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P37253 P37253]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28290]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.86 1.1.1.86]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[ilvB]]-[[ilvH]]-[[ilvC]]-[[leuA]]-[[leuB]]-[[leuC]]-[[leuD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvC_2893681_2894709_-1 ilvC] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed]
−
−	* '''Regulation:'''
−	** for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]
−	** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
−	** expression is stimulated in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed]
−	** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed]
−	** repressed during growth in the presence of branched chain amino acids ([[CodY]], [[T-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
−	** [[CcpA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed]
−	** [[T-box]]: tRNA-controlled [[RNA switch]] that mediates termination/antitermination
−	** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed]
−
−	* '''Additional information:'''
−	** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 21254 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 2878 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 75395 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 36887 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 50694 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:''' pGP521 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455, 12107147, 20509597 22517742 15378759 25755103 25157083 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 156: / Line 156: @@
 =References=
-<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455, 12107147, 20509597 22517742 15378759 25157083 </pubmed>
+<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455, 12107147, 20509597 22517742 15378759 25755103 25157083 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 156: / Line 156: @@
 =References=
-<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455, 12107147, 20509597 22517742 15378759</pubmed>
+<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455, 12107147, 20509597 22517742 15378759 25157083 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 133: / Line 133: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 21254 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 2878 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''

@@ Line 131: / Line 131: @@
 * '''Additional information:'''
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 59: / Line 59: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ilvBHC-leuABCD.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NP3 1NP3] (from ''Escherichia coli'', 53% identity, 71% similarity) {{PubMed|12691757}}

@@ Line 41: / Line 41: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
-{{SubtiWiki category|[[phosphoproteins]]}}
+{{SubtiWiki category|[[phosphoproteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 64: / Line 65: @@
 === Additional information===
 =The protein=
@@ Line 130: / Line 128: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 151: / Line 150: @@
 =References=
-<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455,12107147, 20509597 22517742</pubmed>
+<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455, 12107147, 20509597 22517742 15378759</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28290 ilvC]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val], [http://subtiwiki.uni-goettingen.de/pathways/CoA_synthesis.html Coenzyme A]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ilvC ilvC]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.37

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 86: / Line 82: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
@@ Line 92: / Line 88: @@
 ** phosphorylated on Ser-338 {{PubMed|20509597}}
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 118: / Line 114: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvC_2893681_2894709_-1 ilvC] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed]
+* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed]
-* '''Regulation:''' for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]
+* '''Regulation:'''
 ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
 ** expression is stimulated in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed]
@@ Line 140: / Line 137: @@
 * '''Expression vector:'''
-* '''lacZ fusion:''' pGP521 (in [[pAC5]]), available in [[Stülke]] lab
+* '''lacZ fusion:''' pGP521 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab
 * '''GFP fusion:'''
@@ Line 154: / Line 151: @@
 =References=
-<pubmed>15060025,12193635,19258532,8289305,18641142,15547269,12618455,20935095,15547269,12618455,12107147, 20509597 22517742</pubmed>
+<pubmed>24163341 15060025,12193635,19258532,8289305,18641142, 15547269,12618455,20935095,15547269, 12618455,12107147, 20509597 22517742</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvC_2893681_2894709_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ilvC_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvC_2893681_2894709_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ilvC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28290]]
 |-
 |}