GroEL - Revision history

134.76.70.252 at 14:07, 17 April 2014

2014-04-17T14:07:45Z

134.76.70.252 at 09:38, 17 April 2014

2014-04-17T09:38:01Z

134.76.38.147 at 13:06, 2 April 2014

2014-04-02T13:06:30Z

Jstuelk at 13:00, 5 March 2014

2014-03-05T13:00:53Z

134.76.70.252 at 10:15, 7 January 2014

2014-01-07T10:15:44Z

Jstuelk at 07:33, 16 December 2013

2013-12-16T07:33:05Z

134.76.70.252 at 15:18, 11 November 2013

2013-11-11T15:18:55Z

134.76.70.252 at 12:41, 16 May 2013

2013-05-16T12:41:01Z

134.76.70.252 at 09:36, 14 May 2013

2013-05-14T09:36:20Z

134.76.70.252 at 12:16, 13 May 2013

2013-05-13T12:16:09Z

← Older revision		Revision as of 14:07, 17 April 2014
Line 1:		Line 1:
−	* '''Description:''' chaperonin and co-repressor for [[HrcA]]<br/><br/>	+	{{#bank: GroEL}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''groEL''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| chaperonin, co-repressor for [[HrcA]]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| protein folding and re-folding~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06030 groEL]
−	\|-
−	~~\|colspan="2" style="background:~~#~~FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http~~:~~//subtiwiki.uni-goettingen.de/interact/index.php?protein=GroEL~~ GroEL]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=groEL groEL]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 57 kDa, 4.531~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1632 bp, 544 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[groES]]'', ''[[ydzT/1]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06030 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06030 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06030 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:groEL_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 Expression at a glance]'''   {{PubMed\|22383849}~~}~~<br/>[[Image:groEL_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06030]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[chaperones/ protein folding]]}},~~
−	~~{{SubtiWiki category\|[[heat shock proteins]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}},~~
−	~~[[most abundant proteins]]~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[HrcA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU06030
−
−	~~[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 Expression]~~
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06030&redirect=T BSU06030]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/groESL.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10423]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' acts as co-repressor for [[HrcA]]
−
−	* '''Protein family:''' chaperonin (HSP60) family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−	** phosphorylated on Arg-35, Arg-116, and Arg-282 {{PubMed\|22517742}}
−	** phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:''' [[HrcA]]-[[GroEL]], [[GroEL]]-[[GroES]]
−
−	* '''[[Localization]]:'''
−	** cytoplasm (according to Swiss-Prot)
−	** recruited to the membrane after ethanol stress {{PubMed\|22268681}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06030&redirect=T BSU06030]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P28598 P28598]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU06030]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[groES]]-[[groEL]]'' {{PubMed\|1350777}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 groEL] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|1350776}}
−
−	* '''Regulation:''' induced by heat shock ([[HrcA]]) {{PubMed\|1350777,8576042}}
−
−	* '''Regulatory mechanism:''' [[HrcA]]: transcription repression {{PubMed\|8576042}}
−
−	* '''Additional information:'''
−	** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 14567 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 33441 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−	** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1000 available in [[Jörg Stülke]]'s lab
−	** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP1002 available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Wolfgang Schumann]], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>9303302,1350777,12082092,15378759,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 127: / Line 127: @@
 * '''Additional information:'''
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 63: / Line 63: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/groESL.html]
@@ Line 101: / Line 102: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 44: / Line 44: @@
 {{SubtiWiki category|[[heat shock proteins]]}},
 {{SubtiWiki category|[[essential genes]]}},
-{{SubtiWiki category|[[phosphoproteins]]}}
+{{SubtiWiki category|[[phosphoproteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 68: / Line 69: @@
 === Additional information===
 =The protein=
@@ Line 126: / Line 124: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 150: / Line 149: @@
 =References=
-<pubmed>9303302,1350777,12082092,,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed>
+<pubmed>9303302,1350777,12082092,15378759,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 17: / Line 17: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GroEL GroEL]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=groEL groEL]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 4.531

@@ Line 38: / Line 38: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 90: / Line 86: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
@@ Line 96: / Line 92: @@
 ** phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 123: / Line 119: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 groEL] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|1350776}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1350776}}
 * '''Regulation:''' induced by heat shock ([[HrcA]]) {{PubMed|1350777,8576042}}
@@ Line 136: / Line 132: @@
 * '''Expression vector:'''
-** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1000 available in [[Stülke]] lab
+** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1000 available in [[Jörg Stülke]]'s lab
-** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP1002 available in [[Stülke]] lab
+** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP1002 available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
@@ Line 154: / Line 150: @@
 =References=
-'''Additional publications:''' {{PubMed|22268681}}
+<pubmed>9303302,1350777,12082092,,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06030 groEL]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GroEL GroEL]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GroEL GroEL]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''

@@ Line 30: / Line 30: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:groEL_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:groEL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06030]]
 |-
 |}

@@ Line 25: / Line 25: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[groES]]'', ''[[ydzT/1]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06030 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06030 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06030 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06030 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06030 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06030 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:groEL_context.gif]]