GapA - Revision history

134.76.70.252 at 14:06, 17 April 2014

2014-04-17T14:06:50Z

Show changes

134.76.70.252 at 09:24, 17 April 2014

2014-04-17T09:24:53Z

134.76.38.147 at 14:47, 2 April 2014

2014-04-02T14:47:38Z

Jstuelk at 12:53, 5 March 2014

2014-03-05T12:53:56Z

134.76.70.252 at 08:17, 7 January 2014

2014-01-07T08:17:10Z

134.76.70.252 at 09:22, 12 November 2013

2013-11-12T09:22:53Z

Jstuelk: /* Extended information on the protein */

2013-08-27T08:01:51Z

‎Extended information on the protein

Jstuelk: /* Biological materials */

2013-07-13T15:22:57Z

‎Biological materials

Jstuelk at 15:19, 13 July 2013

2013-07-13T15:19:53Z

134.76.70.252 at 14:36, 16 May 2013

2013-05-16T14:36:11Z

@@ Line 149: / Line 149: @@
 **  The accumulation of the ''[[cggR]]-[[gapA]]'' mRNA is strongly dependent on the presence of the [[YkzW]] peptide, due to stabilization of the mRNA {{PubMed|20444087}}.
 ** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
 =Biological materials =

@@ Line 63: / Line 63: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]
@@ Line 107: / Line 108: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 46: / Line 46: @@
 {{SubtiWiki category|[[essential genes]]}},
 {{SubtiWiki category|[[membrane proteins]]}},
-{{SubtiWiki category|[[phosphoproteins]]}}
+{{SubtiWiki category|[[phosphoproteins]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 68: / Line 69: @@
 === Additional information===
 =The protein=
@@ Line 86: / Line 85: @@
 * '''Kinetic information:''' Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/10799476 PubMed]
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
@@ Line 94: / Line 93: @@
 ** Cys152-Cys156 form intramolecular disulfide in response to disulfide stress (diamide, NaOCl-stress) [http://www.ncbi.nlm.nih.gov/sites/entrez/21749987 PubMed]
-* '''Cofactor(s):''' NAD<sup>+</sup> (does not accept NADP<sup>+</sup>) [http://www.ncbi.nlm.nih.gov/sites/entrez/10799476 PubMed]
+* '''[[Cofactors]]:''' NAD<sup>+</sup> (does not accept NADP<sup>+</sup>) [http://www.ncbi.nlm.nih.gov/sites/entrez/10799476 PubMed]
 * '''Effectors of protein activity:'''
@@ Line 143: / Line 142: @@
 * '''Additional information:'''
-** GapA is one of the most abundant proteins in the cell. In the presence of glucose, there are about 25,000 GapA molecules per cell ([http://www.ncbi.nlm.nih.gov/sites/entrez/12634343 PubMed]).
+** GapA is one of the most abundant proteins in the cell. In the presence of glucose, there are about 25,000 GapA molecules per cell ([http://www.ncbi.nlm.nih.gov/sites/entrez/12634343 PubMed])
 ** The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the ''[[cggR]]'' open reading frame. This results in stable mature ''[[gapA]]'' and ''[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' mRNAs. {{PubMed|11489127}}  The processing event requires the [[Rny|RNase Y]] {{PubMed|19193632}}.
 **  The accumulation of the ''[[cggR]]-[[gapA]]'' mRNA is strongly dependent on the presence of the [[YkzW]] peptide, due to stabilization of the mRNA {{PubMed|20444087}}.
@@ Line 179: / Line 179: @@
 =References=
-<pubmed>22517742 23420519 22740702,23034808 21815947  21749987,12850135,19193632, 18673455 , 20444087 17726680, 16479537, 12622823, 12359717,10799476,17505547,11489127, 12123463,17218307, 12634343, 17142398, 17114254,10559165     </pubmed>
+<pubmed>22517742 23420519 22740702,23034808 21815947  21749987,12850135,19193632, 18673455 , 20444087 17726680, 16479537, 12622823, 12359717,10799476,17505547,11489127, 12123463,17218307, 12634343, 17142398, 17114254,10559165  15378759   </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GapA GapA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gapA gapA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35.7 kDa, 5.03

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33940 gapA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GapA GapA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GapA GapA]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''

@@ Line 150: / Line 150: @@
 =Biological materials =
 * '''Mutant:'''
-** GP592 (''gapA''::''cat''), available in [[Stülke]] lab
+** GP592 (''gapA''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
-** GP597 (''gapA''::''erm''), available in [[Stülke]] lab
+** GP597 (''gapA''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
-** GP703 (''gapA''::''cat'' ''[[gapB]]''::''spec''), available in [[Stülke]] lab
+** GP703 (''gapA''::''cat'' ''[[gapB]]''::''spec''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
-** GM1501 (under p(spac) control), available in [[Stephane Aymerich]] lab
+** GM1501 (under p(spac) control), available in [[Stephane Aymerich]]'s lab
-** 1A1003 ( ''gapA''::''erm''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1003&Search=1A1003 BGSC]
+** 1A1003 ( ''gapA''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1003&Search=1A1003 BGSC]
 * '''Expression vector:'''
-** pGP1424 (expression in  ''B. subtilis'', in [[pBQ200]]) (available in [[Stülke]] lab)
+** pGP1424 (expression in  ''B. subtilis'', in [[pBQ200]]) (available in [[Jörg Stülke]]'s lab)
-** pGP90 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
+** pGP90 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Jörg Stülke]]'s lab)
-** pGP704 (N-terminal His-tag, in [[pWH844]]) (available in [[Stülke]] lab)
+** pGP704 (N-terminal His-tag, in [[pWH844]]) (available in [[Jörg Stülke]]'s lab)
-* '''lacZ fusion:''' pGP506 (in [[pAC7]]), pGP512 (in [[pAC6]]) (available in [[Stülke]] lab)
+* '''lacZ fusion:''' pGP506 (in [[pAC7]]), pGP512 (in [[pAC6]]) (available in [[Jörg Stülke]]'s lab)
 * '''GFP fusion:'''
-* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
-* '''Antibody:''' available in [[Stülke]] lab
+* '''Antibody:''' available in [[Jörg Stülke]]'s lab
 =Labs working on this gene/protein=

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gapA_3481698_3482705_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:gapA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gapA_3481698_3482705_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:gapA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33940]]
 |-
 |}