EzrA - Revision history

Jstuelk at 12:49, 10 May 2015

2015-05-10T12:49:07Z

Jstuelk at 15:54, 10 April 2015

2015-04-10T15:54:18Z

Jstuelk at 09:33, 19 November 2014

2014-11-19T09:33:59Z

Jstuelk: /* Phenotypes of a mutant */

2014-11-19T09:32:56Z

‎Phenotypes of a mutant

Jstuelk at 09:19, 19 November 2014

2014-11-19T09:19:36Z

Jstuelk at 09:19, 19 November 2014

2014-11-19T09:19:00Z

Jstuelk: /* Original Publications */

2014-07-30T11:17:15Z

‎Original Publications

Jstuelk at 17:00, 16 May 2014

2014-05-16T17:00:24Z

134.76.70.252 at 09:48, 17 April 2014

2014-04-17T09:48:33Z

134.76.38.147 at 14:31, 2 April 2014

2014-04-02T14:31:21Z

← Older revision		Revision as of 12:49, 10 May 2015
Line 1:		Line 1:
−	* '''Description:~~''' negative regulator of [[FtsZ]] ring formation <br/><br/>~~	+	{{#bank: EzrA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ezrA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ytwP ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[FtsZ]]-interacting protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| control of [[FtsZ]] ring formation~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29610 ezrA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=EzrA EzrA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 64 kDa, 4.757~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1686 bp, 562 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[braB]]'', ''[[hisJ]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29610 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:ezrA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ezrA_3029729_3031417_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ezrA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29610]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell division]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU29610
−
−	~~===Phenotypes of a mutant ===~~
−	* altered localization of [[FtsZ]] and [[ponA\|PBP1]] {{PubMed\|25403286}}
−	* growth defects, elongated cells {{PubMed\|25403286}}
−	* the ''[[ezrA]]'' mutation is synthetically lethal with a ''[[sepF]]'' mutation {{PubMed\|24218584}}
−	* a ''[[yvcL]] [[ezrA]]'' double mutant grows poorly, and the cells are filamentous {{PubMed\|24097947}}
−	* a ''[[zapA]] [[ezrA]]'' double mutant forms filamentous cells {{PubMed\|16420366}}
−	* depletion of ''[[pdhA]]'' and deletion of ''[[ezrA]]'' have a strong synthetic defect in [[cell division]] {{PubMed\|24825009}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29610&redirect=T BSU29610]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ezrA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13934]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** inhibits [[FtsZ]] polymerization {{PubMed\|25403286}}
−	** recruits [[ponA\|PBP1]] to the division septum {{PubMed\|25403286}}
−	** activates [[PrkC]] {{PubMed\|25845974}}
−
−	* '''Protein family:''' ezrA family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−	** degradation of [[EzrA]] involves [[FtsH]] {{PubMed\|24222488}}
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[EzrA]]-[[UgtP]] {{PubMed\|17662947}}
−	** [[FtsZ]]-[[EzrA]] {{PubMed\|25403286,10449747}}
−	** [[EzrA]]-[[GpsB]] {{PubMed\|18363795}}
−	** [[EzrA]]-[[PonA]] {{PubMed\|18363795}}
−
−	* '''[[Localization]]:'''
−	** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
−	** 1 transmembrane domain at the N-terminus {{PubMed\|25403286}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29610&redirect=T BSU29610]
−
−	* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=4uxv 4UXV] (the 60 kDa cytoplasmic domain) {{PubMed\|25403286}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O34894 O34894]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29610]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[ezrA]]'' {{PubMed\|9387221}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ezrA_3029729_3031417_-1 ezrA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|15317798}}
−
−	* '''Regulation:'''
−	** constitutively expressed {{PubMed\|23701187}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** degradation of [[EzrA]] involves [[FtsH]] {{PubMed\|24222488}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 125 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 495 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A805 ( ''ezrA''::''kan''), {{PubMed\|15101985}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A805&Search=1A805 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~==Reviews==~~
−	~~<pubmed> 19680248 19884039 </pubmed>~~
−	~~==Original Publications==~~
−	<pubmed>10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221 15317798 , 19429628, 18763711 21401734 24222488 23701187 17718511 24218584 24097947 24825009 25068683 25403286 25845974</pubmed>
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 76: / Line 76: @@
 ** inhibits [[FtsZ]] polymerization {{PubMed|25403286}}
 ** recruits [[ponA|PBP1]] to the division septum {{PubMed|25403286}}
 * '''Protein family:''' ezrA family (according to Swiss-Prot)
@@ Line 159: / Line 160: @@
 <pubmed> 19680248  19884039 </pubmed>
 ==Original Publications==
-<pubmed>10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221 15317798 , 19429628, 18763711 21401734 24222488 23701187 17718511 24218584 24097947 24825009 25068683 25403286 </pubmed>
+<pubmed>10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221 15317798 , 19429628, 18763711 21401734 24222488 23701187 17718511 24218584 24097947 24825009 25068683 25403286 25845974</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 53: / Line 53: @@
 ===Phenotypes of a mutant ===
-* altered localization of [[FtsZ]] and
+* altered localization of [[FtsZ]] and [[ponA|PBP1]] {{PubMed|25403286}}
 * the ''[[ezrA]]'' mutation is synthetically lethal with a ''[[sepF]]'' mutation {{PubMed|24218584}}
 * a ''[[yvcL]] [[ezrA]]'' double mutant grows poorly, and the cells are filamentous {{PubMed|24097947}}

@@ Line 53: / Line 53: @@
 ===Phenotypes of a mutant ===
 * the ''[[ezrA]]'' mutation is synthetically lethal with a ''[[sepF]]'' mutation {{PubMed|24218584}}
 * a ''[[yvcL]] [[ezrA]]'' double mutant grows poorly, and the cells are filamentous {{PubMed|24097947}}

@@ Line 73: / Line 73: @@
 * '''Catalyzed reaction/ biological activity:'''
 ** inhibits [[FtsZ]] polymerization {{PubMed|25403286}}
-** recruits [[PBP1|ponA]] to the division septum {{PubMed|25403286}}
+** recruits [[ponA|PBP1]] to the division septum {{PubMed|25403286}}
 * '''Protein family:''' ezrA family (according to Swiss-Prot)

@@ Line 66: / Line 66: @@
 === Additional information===
 =The protein=
@@ Line 75: / Line 72: @@
 * '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:''' ezrA family (according to Swiss-Prot)
@@ Line 95: / Line 94: @@
 * '''[[SubtInteract|Interactions]]:'''
 ** [[EzrA]]-[[UgtP]] {{PubMed|17662947}}
-** [[FtsZ]]-[[EzrA]] {{PubMed|10449747}}
+** [[FtsZ]]-[[EzrA]] {{PubMed|25403286,10449747}}
 ** [[EzrA]]-[[GpsB]] {{PubMed|18363795}}
 ** [[EzrA]]-[[PonA]] {{PubMed|18363795}}
-* '''[[Localization]]:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
+* '''[[Localization]]:'''
-** 1 transmembrane domain at the N-terminus according to SOSUI
+** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
 === Database entries ===
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29610&redirect=T BSU29610]
-* '''Structure:'''
+* '''Structure:'''	[http://www.pdb.org/pdb/explore/explore.do?structureId=4uxv 4UXV] (the 60 kDa cytoplasmic domain) {{PubMed|25403286}}
 * '''UniProt:''' [http://www.uniprot.org/uniprot/O34894 O34894]
@@ Line 157: / Line 157: @@
 <pubmed> 19680248  19884039 </pubmed>
 ==Original Publications==
-<pubmed>10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221 15317798 , 19429628, 18763711 21401734 24222488 23701187 17718511 24218584 24097947 24825009 25068683 </pubmed>
+<pubmed>10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221 15317798 , 19429628, 18763711 21401734 24222488 23701187 17718511 24218584 24097947 24825009 25068683 25403286 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 129: / Line 129: @@
 * '''Additional information:'''
 ** degradation of [[EzrA]] involves [[FtsH]] {{PubMed|24222488}}
 =Biological materials =