BcaP - Revision history

Jstuelk: /* References */

2015-03-18T17:22:31Z

‎References

Jstuelk: /* Biological materials */

2015-02-04T15:01:19Z

‎Biological materials

Jstuelk at 15:00, 4 February 2015

2015-02-04T15:00:41Z

Jstuelk: /* Phenotypes of a mutant */

2015-02-04T14:56:54Z

‎Phenotypes of a mutant

Jstuelk at 14:47, 4 February 2015

2015-02-04T14:47:37Z

Jstuelk at 19:01, 1 February 2015

2015-02-01T19:01:50Z

134.76.70.252 at 11:42, 8 April 2014

2014-04-08T11:42:07Z

134.76.38.147 at 13:17, 2 April 2014

2014-04-02T13:17:48Z

Jstuelk: /* Basic information/ Evolution */

2013-11-28T18:38:39Z

‎Basic information/ Evolution

Jstuelk at 13:04, 29 October 2013

2013-10-29T13:04:28Z

← Older revision		Revision as of 17:22, 18 March 2015
Line 1:		Line 1:
−	* '''Description:''' branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport <br/><br/>	+	{{#bank: BcaP}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''bcaP''~~
−	\|-
−	~~\|style="background:~~#~~ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yhdG ''~~
−	\|-
−	~~\|style="background~~:~~#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| branched-chain amino acid transporter~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| biosynthesis/acquisition of branched-chain amino acids~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09460 bcaP]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=BcaP ~~BcaP]'''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 49 kDa, 9.735~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1395 bp, 465 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yhdF]]'', ''[[yhdH]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09460 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yhdG_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ctrA_1023350_1024747_1 Expression at a glance]'''   {{PubMed\|22383849~~}~~}<br/>[[Image:bcaP_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09460]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[transporters/ other]]}},~~
−	~~{{SubtiWiki category\|[[biosynthesis/ acquisition of amino acids]]}},~~
−	~~{{SubtiWiki category\|[[utilization of amino acids]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CodY regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU09460
−
−	~~===Phenotypes of a mutant ===~~
−	* no phenotype for the single mutant, the triple ''[[bcaP]] [[brnQ]] [[braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations {{PubMed\|25645558}}
−	* resistant to growth inhibition by high concentrations of threonine {{PubMed\|25645558}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU09460&redirect=T BSU09460]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13013]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** high affinity uptake of isoleucine and valine {{PubMed\|25645558}}
−
−	* '''Protein family:'''
−	** [[APC superfamily of transport proteins\|amino acid, polyamine, organocation (APC) superfamily of transporters]], [http://www.tcdb.org/search/result.php?tc=2.A.3 TC 2.A.3]
−
−	* '''Paralogous protein(s):''' [[YfnA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−	** for Ile: Km ≈ 4.1 μM; Vmax at 26°C was ~15 nmol/min/ mg protein {{PubMed\|25645558}}
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU09460&redirect=T BSU09460]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O07576 O07576]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09460]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ctrA_1023350_1024747_1 bcaP] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed\|12618455,21097623}}
−
−	* '''Regulatory mechanism:'''
−	** [[CodY]]: transcription repression {{PubMed\|12618455,21097623,18083814,21699902}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' BKE09460 (''[[bcaP]]''::''erm'', available in the BGSC and in [[Fabian Commichau]]'s and [[Jörg Stülke]]'s labs)
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>18083814,12618455, 20935095, 18763711, 21097623 21699902 25645558</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 126: / Line 126: @@
 =Biological materials =
-* '''Mutant:''' BKE09460 (''[[bcaP]]''::''erm'', available in the BGSC and in [[Fabian Commichau]]'s lab)
+* '''Mutant:''' BKE09460 (''[[bcaP]]''::''erm'', available in the BGSC and in [[Fabian Commichau]]'s and [[Jörg Stülke]]'s labs)
 * '''Expression vector:'''

@@ Line 1: / Line 1: @@
-* '''Description:''' branched-chain amino acid transporter, major transporter for isoleucine and valine <br/><br/>
+* '''Description:''' branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-

@@ Line 55: / Line 55: @@
 ===Phenotypes of a mutant ===
 * no phenotype for the single mutant, the triple ''[[bcaP]] [[brnQ]] [[braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations {{PubMed|25645558}}
 === Database entries ===

@@ Line 1: / Line 1: @@
-* '''Description:''' branched-chain amino acid transporter <br/><br/>
+* '''Description:''' branched-chain amino acid transporter, major transporter for isoleucine and valine <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-
@@ Line 54: / Line 54: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 69: / Line 70: @@
 * '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:'''
@@ Line 78: / Line 80: @@
 * '''Kinetic information:'''
 * '''Domains:'''
@@ Line 140: / Line 143: @@
 =References=
-<pubmed>18083814,12618455, 20935095, 18763711, 21097623 21699902 </pubmed>
+<pubmed>18083814,12618455, 20935095, 18763711, 21097623 21699902 25645558</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 63: / Line 63: @@
 === Additional information===
 =The protein=
@@ Line 113: / Line 110: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ctrA_1023350_1024747_1 bcaP] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 125: / Line 122: @@
 =Biological materials =
-* '''Mutant:'''
+* '''Mutant:''' BKE09460 (''[[bcaP]]''::''erm'', available in the BGSC and in [[Fabian Commichau]]'s lab)
 * '''Expression vector:'''

@@ Line 14: / Line 14: @@
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09460 bcaP]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 9.735

@@ Line 70: / Line 70: @@
 * '''Catalyzed reaction/ biological activity:'''
-* '''Protein family:''' amino acid-polyamine-organocation (APC) superfamily (according to Swiss-Prot)
+* '''Protein family:'''
 * '''Paralogous protein(s):''' [[YfnA]]

@@ Line 34: / Line 34: @@
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