AcuA - Revision history

134.76.38.147 at 14:31, 2 April 2014

2014-04-02T14:31:44Z

134.76.70.252 at 14:19, 16 May 2013

2013-05-16T14:19:25Z

134.76.70.252 at 11:01, 14 May 2013

2013-05-14T11:01:38Z

134.76.70.252 at 13:37, 13 May 2013

2013-05-13T13:37:07Z

Jstuelk: /* References */

2012-08-21T15:46:01Z

‎References

134.76.70.252 at 14:59, 7 August 2012

2012-08-07T14:59:39Z

134.76.70.252 at 11:10, 20 April 2012

2012-04-20T11:10:15Z

134.76.70.252 at 15:25, 16 April 2012

2012-04-16T15:25:24Z

Jstuelk: /* Biological materials */

2011-07-26T08:39:05Z

‎Biological materials

Jstuelk at 10:13, 16 July 2011

2011-07-16T10:13:49Z

← Older revision		Revision as of 14:31, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' protein acetylase for the control of [[AcsA]] activity <br/><br/>~~	+	{{#bank: AcuA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''acuA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| Gcn5-related N-acetyltransferase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| control of [[AcsA]] activity~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29690 acuA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 24 kDa, 5.028~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 630 bp, 210 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[acsA]]'', ''[[acuB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29690 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:acuA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acuA_3040092_3040724_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:acuA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29690]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}},~~
−	~~{{SubtiWiki category\|[[utilization of lipids]]}},~~
−	~~{{SubtiWiki category\|[[protein modification]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU29690
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acuABC.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10369]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' acetylates (and thereby inactivates) [[AcsA]] on Lys-549 [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]
−
−	* '''Protein family:''' acetyltransferase family (according to Swiss-Prot) Gcn5-relatedN-acetyltransferase [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed]
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39065 P39065]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29690]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[acuA]]-[[acuB]]-[[acuC]]'' {{PubMed\|7934817}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acuA_3040092_3040724_1 acuA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigA]] {{PubMed\|7913927}}
−
−	* '''Regulation:'''
−	** repressed by glucose (3.4-fold) ([[CcpA]]) {{PubMed\|7913927,12850135}}
−
−	* '''Regulatory mechanism:'''
−	** [[CcpA]]: transcription repression {{PubMed\|7913927}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP1211 (''acuA''::''kan''), available in [[Jörg Stülke]]'s lab
−	** GP1255 (''[[acuA]]''::''kan'' ''[[yjcK]]''::''cat'' ''[[ynaD]]''::''spec'' ''[[yoaA]]''::''tet''), available in [[Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>7913927, 22900538,16855235,7934817, 19136592, 18487328, 12850135 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acuA_3040092_3040724_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:acuA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acuA_3040092_3040724_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:acuA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29690]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acsA]]'', ''[[acuB]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29690 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29690 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:acuA_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[AcsA]] activity
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU29690 acuA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29690 acuA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.028
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acsA]]'', ''[[acuB]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14947&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29690 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:acuA_context.gif]]

@@ Line 143: / Line 143: @@
 =References=
-<pubmed>7913927,,16855235,7934817, 19136592, 18487328, 12850135 </pubmed>
+<pubmed>7913927, 22900538,16855235,7934817, 19136592, 18487328, 12850135 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 99: / Line 99: @@
 * '''Operon:''' ''[[acuA]]-[[acuB]]-[[acuC]]'' {{PubMed|7934817}}
-* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7913927}}
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=acuA_3040092_3040724_1 acuA] {{PubMed|22383849}}
 * '''Regulation:'''

@@ Line 112: / Line 112: @@
 * '''Mutant:'''
-** GP1211 (''acuA''::''kan''), available in [[Stülke]] lab
+** GP1211 (''acuA''::''kan''), available in [[Jörg Stülke]]'s lab
 * '''Expression vector:'''

@@ Line 82: / Line 82: @@
 * '''[[SubtInteract|Interactions]]:'''
-* '''Localization:'''
+* '''[[Localization]]:'''
 === Database entries ===