SubtiWiki - User contributions [en]

Sven Halbedel

2021-10-28T13:57:24Z

SHalbedel: /* Open positions */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Principal investigator
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Coordination of peptidoglycan biosynthesis with [[cell division]]
*Listeria virulence and molecular infection genetics
*Whole genome sequencing based infection epidemiology of listeriosis
*Protein targeting to, in and across membranes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

Currently (closing date 15th November 2021) we offer a position for a Postdoc: [https://www.jobvector.de/jobs-stellenangebote/biologie-life-sciences/wissenschaftliche-r-mitarbeiter-in/wissenschaftliche-mitarbeiterin-wissenschaftlichen-postdoc-161308.html]

'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2021-02-22T12:40:31Z

SHalbedel:

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Principal investigator
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Coordination of peptidoglycan biosynthesis with [[cell division]]
*Listeria virulence and molecular infection genetics
*Whole genome sequencing based infection epidemiology of listeriosis
*Protein targeting to, in and across membranes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

Currently (closing date 15th March 2021) we offer a position for a PhD student: [https://www.jobvector.de/jobs-stellenangebote/biologie-life-sciences/wissenschaftliche-r-mitarbeiter-in/biologe-biotechnologe-humanbiologe-absolvent-molecular-life-sciences-doktorand-144599.html]

'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2021-01-20T14:51:22Z

SHalbedel:

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Principal investigator
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Coordination of peptidoglycan biosynthesis with [[cell division]]
*Listeria virulence and molecular infection genetics
*Whole genome sequencing based infection epidemiology of listeriosis
*Protein targeting to, in and across membranes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

Currently (closing date 31st Jan 2021) we offer a position for a PhD student: [https://www.jobvector.de/jobs-stellenangebote/biologie-life-sciences/wissenschaftliche-r-mitarbeiter-in/doktorandin-doktorand-144599.html]

'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2021-01-20T14:50:29Z

SHalbedel:

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Principal investigator
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Coordination of peptidoglycan biosynthesis with [[cell division]]
*Listeria virulence and molecular infection genetics
*Whole genome sequencing based infection epidemiology of listeriosis
*Protein targeting to, in and across membranes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

Currently we offer a position for a PhD student: [https://www.jobvector.de/jobs-stellenangebote/biologie-life-sciences/wissenschaftliche-r-mitarbeiter-in/doktorandin-doktorand-144599.html]

'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2020-05-15T16:11:13Z

SHalbedel: /* Research Interests */

Sven Halbedel

2020-05-15T16:08:05Z

SHalbedel: /* Open positions */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Principal investigator
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Penicillin binding proteins and peptidoglycan biosynthesis
*Spatiotemporal coordination of bacterial [[cell division]]
*Listeria virulence
*Identification of Listeria monocytogenes virulence genes by whole genome sequencing of human isolates
*Protein targeting to, in and across membranes
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2020-05-15T16:07:03Z

SHalbedel: /* Sven Halbedel */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Principal investigator
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Penicillin binding proteins and peptidoglycan biosynthesis
*Spatiotemporal coordination of bacterial [[cell division]]
*Listeria virulence
*Identification of Listeria monocytogenes virulence genes by whole genome sequencing of human isolates
*Protein targeting to, in and across membranes
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently (deadline 19.02.2020), we offer a position for a '''PhD student''': [https://interamt.de/koop/app/stelle?id=564723]
'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2020-01-23T13:58:09Z

SHalbedel: /* Open positions */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Penicillin binding proteins and peptidoglycan biosynthesis
*Spatiotemporal coordination of bacterial [[cell division]]
*Listeria virulence
*Identification of Listeria monocytogenes virulence genes by whole genome sequencing of human isolates
*Protein targeting to, in and across membranes
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently (deadline 19.02.2020), we offer a position for a '''PhD student''': [https://interamt.de/koop/app/stelle?id=564723]
'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2016-09-23T08:06:58Z

SHalbedel: /* Research Interests */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]]- and [[GpsB]]- like proteins
*Penicillin binding proteins and peptidoglycan biosynthesis
*Spatiotemporal coordination of bacterial [[cell division]]
*Listeria virulence
*Identification of Listeria monocytogenes virulence genes by whole genome sequencing of human isolates
*Protein targeting to, in and across membranes
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently (deadline 23.10.2016), we offer a position for a '''PhD student''': [http://www.jobvector.de/jobs-stellenangebote/biologie-life-sciences/forschung-entwicklung/doktorandin-doktorand-80024.html]
'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2016-09-23T08:03:45Z

SHalbedel: /* Research Interests */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[DivIVA] and [GpsB]- like proteins
*Listeria virulence
*Spatiotemporal coordination of bacterial [[cell division]]
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently (deadline 23.10.2016), we offer a position for a '''PhD student''': [http://www.jobvector.de/jobs-stellenangebote/biologie-life-sciences/forschung-entwicklung/doktorandin-doktorand-80024.html]
'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2016-09-23T08:02:44Z

SHalbedel: /* Open positions */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]] like proteins
*Listeria virulence
*Spatiotemporal coordination of bacterial [[cell division]]
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently (deadline 23.10.2016), we offer a position for a '''PhD student''': [http://www.jobvector.de/jobs-stellenangebote/biologie-life-sciences/forschung-entwicklung/doktorandin-doktorand-80024.html]
'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2015-11-27T13:04:52Z

SHalbedel: /* Open positions */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]] like proteins
*Listeria virulence
*Spatiotemporal coordination of bacterial [[cell division]]
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently we offer a position for a '''PhD student''': [http://www.rki.de/DE/Content/Service/Stellen/Angebote/2015/113_15.html]
'''Back to [[Labs working on Bacillus]]'''

DivIVA

2014-04-05T18:51:59Z

SHalbedel: /* Biological materials */

* '''Description:''' curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, cell-division initiation protein (septum placement), part of the Min system (with [[MinC]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420 divIVA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DivIVA DivIVA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15420 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:divIVA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[Spo0A regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
* Deletion of ''divIVA'' leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed]
* A ''divIVA'' mutant has a severe [[sporulation]] defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15420&redirect=T BSU15420]

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
** curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum
** DivIVA is required for polar localisation of [[MinC]]-[[MinD]] via [[MinJ]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed]
** It also recruits [[RacA]] to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''[[Domains]]:'''
** the first 60 amino acids constitute a conserved lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
** the C-terminal domain is less conserved
** multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain {{PubMed|18388125}} {{PubMed|23264578}}

* '''Modification:'''
** phosphorylated on Arg-102 {{PubMed|22517742}}
** The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed]
** DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed][http://www.ncbi.nlm.nih.gov/sites/entrez/22211696 PubMed]

* '''[[Cofactors]]:''' not known

* '''Effectors of protein activity:''' not known

* '''[[SubtInteract|Interactions]]:'''
** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}, [[MinJ]] binds to the N-terminal lipid-binding domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[RacA]], [[RacA]] binds to the C-terminal domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[DivIVA]] {{PubMed|23264578}}
** [[Maf]]-[[DivIVA]] {{PubMed|21564336}}
** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}
** [[DivIVA]]-[[SecA]] {{PubMed|24592260}}

* '''[[Localization]]:'''
** DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].
** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}
** membrane targeting requires [[SecA]] {{PubMed|24592260}}

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15420&redirect=T BSU15420]

* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 divIVA] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
** constitutively expressed {{PubMed|23701187}}

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** 4041 (''divIVA''::''tet''), available in [[Leendert Hamoen]]'s, [[Jörg Stülke]]'s, and [[Sven Halbedel]] 's lab

* '''Expression vector:''' DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Sven Halbedel]]'s and [[Jörg Stülke]]'s labs

* '''Antibody:''' A polyclonal anti-DivIVA antiserum generated in rabbit is described here {{PubMed|11445541}}.

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

[[Sven Halbedel]], Robert Koch Institute [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 19654604, 19884039 22722244 </pubmed>

==Original Publications==
<pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 24391905 24592260 24600441 </pubmed>
[[Category:Protein-coding genes]]

PonA

2013-09-09T14:21:10Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' [[penicillin-binding protein]] 1A/1B 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ponA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[penicillin-binding protein]] 1A/1B
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || bifunctional glucosyltransferase/ transpeptidase
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22320 ponA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PonA PonA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 99 kDa, 4.752
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2742 bp, 914 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[recU]]'', ''[[ypoC]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22320 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:ponA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ponA_2341444_2344188_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ponA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22320]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell wall synthesis]]}},
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
{{SubtiWiki category|[[membrane proteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[SigM regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU22320

===Phenotypes of a mutant ===
* prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a ''[[mreB]]'' mutation [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed]
* deletion of ''ponA'' restores growth and normal shape of a ''[[yvcK]]'' mutant on gluconeogenic carbon sources {{PubMed|21320184}}

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/recU-ponA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10954]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[EzrA]]-[[PonA]] {{PubMed|18363795}}
** [[GpsB]]-[[PonA]] {{PubMed|18363795}}

* '''[[Localization]]:'''
** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
** localizes to the spore septum during sporulation {{PubMed|15758244,15262952}}
** localizes to the vegetative septum {{PubMed|15262952}}, this depends on [[YvcK]] {{PubMed|21320184}}

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39793 P39793]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22320]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[recU]]-[[ponA]]'' {{PubMed|7814321}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ponA_2341444_2344188_1 ponA] {{PubMed|22383849}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|22211522}}, [[SigM]] {{PubMed|18179421}}

* '''Regulation:''' constitutive during vegetative growth [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab

* '''Antibody:'''

=Labs working on this gene/protein=

[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed>21371139 </pubmed>
==Original publications==
<pubmed>8606187,15262952,9721295,10322023,19192185,7814321,18363795,14731276, 21320184,18763711 19192185, 18179421 23531131 18363795</pubmed>

[[Category:Protein-coding genes]]

DivIVA

2013-09-08T17:42:37Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, cell-division initiation protein (septum placement), part of the Min system (with [[MinC]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420 divIVA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DivIVA DivIVA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15420 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:divIVA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[Spo0A regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed] A divIVA mutant has a severe sporulation defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
** curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum
** DivIVA is required for polar localisation of [[MinC]]-[[MinD]] via [[MinJ]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed]
** It also recruits [[RacA]] to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** the first 60 amino acids constitute a conserved lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
** the C-terminal domain is less conserved
** multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain {{PubMed|18388125}} {{PubMed|23264578}}

* '''Modification:'''
** phosphorylated on Arg-102 {{PubMed|22517742}}
** The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed]
** DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed][http://www.ncbi.nlm.nih.gov/sites/entrez/22211696 PubMed]

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''[[SubtInteract|Interactions]]:'''
** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}, [[MinJ]] binds to the N-terminal lipid-binding domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[RacA]], [[RacA]] binds to the C-terminal domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[DivIVA]] {{PubMed|23264578}}
** [[Maf]]-[[DivIVA]] {{PubMed|21564336}}
** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}

* '''[[Localization]]:'''
** DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].
** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}

=== Database entries ===

* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 divIVA] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
** constitutively expressed {{PubMed|23701187}}

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:''' DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

[[Sven Halbedel]], Robert Koch Institute [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 19654604, 19884039 22722244 </pubmed>

==Original Publications==
<pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 </pubmed>
[[Category:Protein-coding genes]]

DivIVA

2013-09-08T17:39:04Z

SHalbedel: /* Biological materials */

* '''Description:''' curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, cell-division initiation protein (septum placement), part of the Min system (with [[MinC]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420 divIVA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DivIVA DivIVA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15420 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:divIVA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[Spo0A regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed] A divIVA mutant has a severe sporulation defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
** curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum
** DivIVA is required for polar localisation of [[MinC]]-[[MinD]] via [[MinJ]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed]
** It also recruits [[RacA]] to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** the first 60 amino acids constitute a conserved lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
** the C-terminal domain is less conserved
** multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:'''
** phosphorylated on Arg-102 {{PubMed|22517742}}
** The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed]
** DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed][http://www.ncbi.nlm.nih.gov/sites/entrez/22211696 PubMed]

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''[[SubtInteract|Interactions]]:'''
** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}, [[MinJ]] binds to the N-terminal lipid-binding domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[RacA]], [[RacA]] binds to the C-terminal domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[DivIVA]] {{PubMed|23264578}}
** [[Maf]]-[[DivIVA]] {{PubMed|21564336}}
** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}

* '''[[Localization]]:'''
** DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].
** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}

=== Database entries ===

* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 divIVA] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
** constitutively expressed {{PubMed|23701187}}

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:''' DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

[[Sven Halbedel]], Robert Koch Institute [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 19654604, 19884039 22722244 </pubmed>

==Original Publications==
<pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 </pubmed>
[[Category:Protein-coding genes]]

DivIVA

2013-09-08T17:36:29Z

SHalbedel: /* Labs working on this gene/protein */

* '''Description:''' curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, cell-division initiation protein (septum placement), part of the Min system (with [[MinC]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420 divIVA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DivIVA DivIVA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15420 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:divIVA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15420]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[Spo0A regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed] A divIVA mutant has a severe sporulation defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
** curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum
** DivIVA is required for polar localisation of [[MinC]]-[[MinD]] via [[MinJ]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed]
** It also recruits [[RacA]] to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** the first 60 amino acids constitute a conserved lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
** the C-terminal domain is less conserved
** multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:'''
** phosphorylated on Arg-102 {{PubMed|22517742}}
** The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed]
** DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed][http://www.ncbi.nlm.nih.gov/sites/entrez/22211696 PubMed]

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''[[SubtInteract|Interactions]]:'''
** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}, [[MinJ]] binds to the N-terminal lipid-binding domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[RacA]], [[RacA]] binds to the C-terminal domain of [[DivIVA]] {{PubMed|23264578}}
** [[DivIVA]]-[[DivIVA]] {{PubMed|23264578}}
** [[Maf]]-[[DivIVA]] {{PubMed|21564336}}
** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}

* '''[[Localization]]:'''
** DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].
** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}

=== Database entries ===

* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 divIVA] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
** constitutively expressed {{PubMed|23701187}}

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

[[Sven Halbedel]], Robert Koch Institute [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 19654604, 19884039 22722244 </pubmed>

==Original Publications==
<pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 </pubmed>
[[Category:Protein-coding genes]]

GpsB

2013-09-06T18:39:46Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' required for removal of [[ponA|PBP1]] from the cell pole after completion of cell pole maturation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gpsB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypsB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell division protein, member of the divisome
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || cell division, cell elongation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22180 gpsB]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 5.629
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 294 bp, 98 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnpB]]'', ''[[ypsA]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22180 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:ypsB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gpsB_2331779_2332075_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:gpsB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22180]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU22180

===Phenotypes of a mutant ===
* synthetic lethal phenotype with a ''[[ftsA]]'' mutation {{PubMed|18776011}}; synthetic sick phenotype when combined with an ''[[ezrA]]'' mutation {{PubMed|18363795}}

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11454]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[DivIVA]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' contains a [[DivIVA]]-like N-terminal domain

* '''Modification:''' phosphorylation on (Ser-73 OR Thr-75) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[GpsB]]-[[EzrA]] {{PubMed|18363795}}
** [[GpsB]]-[[PonA]] {{PubMed|18363795}}
** [[GpsB]]-[[MreC]] {{PubMed|18363795}}

* '''[[Localization]]:'''
** dynamic, shuttles between lateral membrane and the divison septum [http://www.ncbi.nlm.nih.gov/sites/entrez/18363795 PubMed], localizes to the divisome after Z-ring formation {{PubMed|18776011}}

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P50839 P50839]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22180]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gpsB_2331779_2332075_-1 gpsB] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** 1A934 ( ''gpsB''::''kan''), {{PubMed|18776011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
** 1A970 ( ''gpsB''::''kan''), {{PubMed|18776011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A970&Search=1A970 BGSC]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>18776011,, 19429628, 17218307, 18363795 </pubmed>

[[Category:Protein-coding genes]]

EzrA

2013-09-06T18:38:19Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' negative regulator of [[FtsZ]] ring formation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ezrA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytwP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[FtsZ]]-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[FtsZ]] ring formation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29610 ezrA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/EzrA EzrA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 4.757
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1686 bp, 562 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[braB]]'', ''[[hisJ]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29610 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:ezrA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ezrA_3029729_3031417_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ezrA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29610]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29610

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ezrA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13934]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' ezrA family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[EzrA]]-[[UgtP]] {{PubMed|17662947}}
** [[FtsZ]]-[[EzrA]] {{PubMed|10449747}}
** [[EzrA]]-[[GpsB]] {{PubMed|18363795}}
** [[EzrA]]-[[PonA]] {{PubMed|18363795}}

* '''[[Localization]]:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
** 1 transmembrane domain at the N-terminus according to SOSUI

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34894 O34894]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29610]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[ezrA]]'' {{PubMed|9387221}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ezrA_3029729_3031417_-1 ezrA] {{PubMed|22383849}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15317798}}

* '''Regulation:'''
** constitutively expressed {{PubMed|23701187}}

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** 1A805 ( ''ezrA''::''kan''), {{PubMed|15101985}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A805&Search=1A805 BGSC]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

==Reviews==
<pubmed> 19680248 19884039 </pubmed>
==Original Publications==
<pubmed>10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221 15317798 , 19429628, 18763711 21401734 23701187</pubmed>

[[Category:Protein-coding genes]]

EzrA

2013-09-06T09:22:54Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' negative regulator of [[FtsZ]] ring formation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ezrA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytwP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[FtsZ]]-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[FtsZ]] ring formation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29610 ezrA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/EzrA EzrA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 4.757
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1686 bp, 562 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[braB]]'', ''[[hisJ]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29610 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:ezrA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ezrA_3029729_3031417_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ezrA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29610]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29610

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ezrA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13934]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' ezrA family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[EzrA]]-[[UgtP]] {{PubMed|17662947}}
** [[FtsZ]]-[[EzrA]] {{PubMed|10449747}}

* '''[[Localization]]:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
** 1 transmembrane domain at the N-terminus accodring to SOSUI

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34894 O34894]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29610]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[ezrA]]'' {{PubMed|9387221}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ezrA_3029729_3031417_-1 ezrA] {{PubMed|22383849}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15317798}}

* '''Regulation:'''
** constitutively expressed {{PubMed|23701187}}

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** 1A805 ( ''ezrA''::''kan''), {{PubMed|15101985}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A805&Search=1A805 BGSC]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

==Reviews==
<pubmed> 19680248 19884039 </pubmed>
==Original Publications==
<pubmed>10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221 15317798 , 19429628, 18763711 21401734 23701187</pubmed>

[[Category:Protein-coding genes]]

GpsB

2013-03-19T14:50:15Z

SHalbedel:

* '''Description:''' required for removal of [[ponA|PBP1]] from the cell pole after completion of cell pole maturation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gpsB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypsB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell division protein, member of the divisome
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || cell division, cell elongation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22180 gpsB]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 5.629
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 294 bp, 98 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnpB]]'', ''[[ypsA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14135]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ypsB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gpsB_2331779_2332075_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:gpsB_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU22180

===Phenotypes of a mutant ===
* synthetic lethal phenotype with a ''[[ftsA]]'' mutation {{PubMed|18776011}}; synthetic sick phenotype when combined with an ''[[ezrA]]'' mutation {{PubMed|18363795}}

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11454]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[DivIVA]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' contains a [[DivIVA]]-like N-terminal domain

* '''Modification:''' phosphorylation on (Ser-73 OR Thr-75) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''
** dynamic, shuttles between lateral membrane and the divison septum [http://www.ncbi.nlm.nih.gov/sites/entrez/18363795 PubMed], localizes to the divisome after Z-ring formation {{PubMed|18776011}}

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P50839 P50839]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22180]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gpsB_2331779_2332075_-1 gpsB] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** 1A934 ( ''gpsB''::''kan''), {{PubMed|18776011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
** 1A970 ( ''gpsB''::''kan''), {{PubMed|18776011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A970&Search=1A970 BGSC]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>18776011,, 19429628, 17218307, 18363795 </pubmed>

[[Category:Protein-coding genes]]

Sven Halbedel

2013-02-21T12:31:05Z

SHalbedel: /* Sven Halbedel */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]
*Homepage: [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]] like proteins
*Listeria virulence
*Spatiotemporal coordination of bacterial [[cell division]]
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently we offer a position for a '''PhD student''': [http://www.vaam.uni-halle.de/pdf/PhD_RKI.pdf] and for a '''master student''':[http://www.career.tu-braunschweig.de/cms/cs/2012/06/Abschlussarbeit_Molekulare_Mikrobiologie_Robert-Koch-Institut_20120625.pdf]

'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2012-09-11T12:03:48Z

SHalbedel: /* Looking for */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]] like proteins
*Listeria virulence
*Spatiotemporal coordination of bacterial [[cell division]]
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Minimal genomes

==Open positions==
*Interested students from all levels of education are encouraged to ask for possibilities to join.

*Currently we offer a position for a '''PhD student''': [http://www.vaam.uni-halle.de/pdf/PhD_RKI.pdf] and for a '''master student''':[http://www.career.tu-braunschweig.de/cms/cs/2012/06/Abschlussarbeit_Molekulare_Mikrobiologie_Robert-Koch-Institut_20120625.pdf]

'''Back to [[Labs working on Bacillus]]'''

RacA

2012-07-18T11:17:01Z

SHalbedel:

* '''Description:''' [[cell division]] protein: attaches the chromosome to the cell pole at the onset of [[sporulation]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''racA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywkC ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[cell division]] protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || [[cell division]]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RacA RacA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 9.514
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 552 bp, 184 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywkD]]'', ''[[ywkB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15720]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywkC_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=racA_3798789_3799343_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:racA_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[SigH regulon]]}},
{{SubtiWiki regulon|[[Spo0A regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU37030

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/racA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11314]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' racA family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[RacA]] is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of [[SubtInteract|interactions]] {{PubMed|21630458}}
** [[RacA]]-[[DisA]]
** [[RacA]]-[[DivIVA]] {{PubMed|19478798}}

* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P45870 P45870]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37030]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''racA'' (according to [http://dbtbs.hgc.jp/COG/prom/racA.html DBTBS])

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=racA_3798789_3799343_1 racA] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigH]] {{PubMed|12493822}}

* '''Regulation:'''
** expressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|12493822,14651647,15687200}}

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription activation {{PubMed|12493822,14651647,15687200}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>15780934,19478798,12493822,12950914,21630458,14651647,15687200, 12493822 </pubmed>

[[Category:Protein-coding genes]]

Sven Halbedel

2012-06-27T11:02:32Z

SHalbedel: /* Research Interests */

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*[[DivIVA]] like proteins
*Listeria virulence
*Spatiotemporal coordination of bacterial [[cell division]]
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Minimal genomes

==Looking for==
*Interested students from all levels of education are encouraged to ask for possibilities to join. Currently we are looking for a master student with a keen interest in molecular microbiology:[http://www.career.tu-braunschweig.de/cms/cs/2012/06/Abschlussarbeit_Molekulare_Mikrobiologie_Robert-Koch-Institut_20120625.pdf]

'''Back to [[Labs working on Bacillus]]'''

Sven Halbedel

2012-06-27T11:01:12Z

SHalbedel:

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: [mailto:halbedels@rki.de send mail]

== Publications==
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Halbedel%2BS%22%5Bau%5D&dispmax=50 Pubmed]

==Research Interests==
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Spatiotemporal coordination of bacterial [[cell division]]
*[[DivIVA]] like proteins
*Minimal genomes
*Listeria virulence

==Looking for==
*Interested students from all levels of education are encouraged to ask for possibilities to join. Currently we are looking for a master student with a keen interest in molecular microbiology:[http://www.career.tu-braunschweig.de/cms/cs/2012/06/Abschlussarbeit_Molekulare_Mikrobiologie_Robert-Koch-Institut_20120625.pdf]

'''Back to [[Labs working on Bacillus]]'''

DivIVA

2012-05-16T13:59:34Z

SHalbedel:

* '''Description:''' cell-division initiation protein (septum placement), part of the Min system (with [[MinC]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DivIVA DivIVA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:divIVA_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[Spo0A regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed] A divIVA mutant has a severe sporulation defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:'''
** phosphorylated on Arg-102 {{PubMed|22517742}}
** The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed]
** DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed][http://www.ncbi.nlm.nih.gov/sites/entrez/22211696 PubMed]

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''[[SubtInteract|Interactions]]:'''
** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}
** [[DivIVA]]-[[RacA]], [[DivIVA]]-[[DivIVA]]
** [[Maf]]-[[DivIVA]] {{PubMed|21564336}}
** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}

* '''[[Localization]]:'''
** DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].
** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}

=== Database entries ===

* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 divIVA] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 19654604, 19884039 </pubmed>
==Original Publications==
<pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742</pubmed>
[[Category:Protein-coding genes]]

DivIVA

2012-04-24T15:12:26Z

SHalbedel:

* '''Description:''' cell-division initiation protein (septum placement), part of the Min system (with [[MinC]], [[MinD]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DivIVA DivIVA]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:divIVA_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[Spo0A regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed] A divIVA mutant has a severe sporulation defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:'''
** phosphorylated on Arg-102 {{PubMed|22517742}}
** The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed]
** DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed][http://www.ncbi.nlm.nih.gov/sites/entrez/22211696 PubMed]

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''[[SubtInteract|Interactions]]:'''
** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}
** [[DivIVA]]-[[RacA]], [[DivIVA]]-[[DivIVA]]
** [[Maf]]-[[DivIVA]] {{PubMed|21564336}}

* '''[[Localization]]:'''
** DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].
** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}

=== Database entries ===

* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=divIVA_1612521_1613015_1 divIVA] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 19654604, 19884039 </pubmed>
==Original Publications==
<pubmed>19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742</pubmed>
[[Category:Protein-coding genes]]

Wish List

2011-11-22T12:08:22Z

SHalbedel:

SubtiWiki is all about making a useful tool for the ''Bacillus'' community. It is thus very important to receive feedback on what the community needs.

We believe that there are many types of users, with different requirements. For example:

* You could be a molecular biologist wanting to know the sequence of your gene of interest to make a PCR
* You find a reference to a gene in a paper you are reading and are interested to know more about that gene
* You have some hypotheses in mind and want to travel around the wiki, gathering some facts to get inspired
* You have a piece of information that you need almost daily and want to make it available not only to you, but also your group or to the whole community

In all of these cases, you will need different kind of information. The way you want it to be presented would differ too.

The wish list below takes into consideration all the possible users. Please add your wishes on what you would like to see.

= Wish list =

* Would it be possible to have a BLAST tool implemented in Subtiwiki?
* Überarbeitung der [[rsmY]]-Seite, da fehlt fast alles
* [[Genes_controlled_by_SigA | Extraseite: Liste der sigA kontrollierten Gene bearbeiten]]
* The search should not be case sensitive
* It should be possible to get DNA sequences from intergenic regions, not only 200 bp up- and downstream of the gene
* For all fused and splitted genes, there should be a description of the fate (history of the annotation procedure). See for example [[YqbL]]
* Eine Einteilung der Genprodukte in funktionelle Gruppe ähnlich denen in der alten SubtiList wäre schön.

Sven Halbedel

2011-06-15T15:19:15Z

SHalbedel: /* Looking for */

DivIVA

2010-05-28T08:42:39Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed] A divIVA mutant has a severe sporulation defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed].
The DivIVA from Streptococcus pneumoniae is phosphorylated at a threonine side chain by the Ser/Thr protein kinase Sktp1. [http://www.ncbi.nlm.nih.gov/sites/entrez/20453092 PubMed]

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''Interactions:''' ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}, [[DivIVA]]-[[RacA]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''divIVA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 19654604, 19884039 </pubmed>
==Original Publications==
<pubmed>19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 </pubmed>
[[Category:Protein-coding genes]]

SecA

2009-12-14T10:17:34Z

SHalbedel: /* The protein */

* '''Description:''' preprotein translocase subunit (ATPase) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''secA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''div, div-341, ts-341 ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || preprotein translocase subunit (ATPase)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || protein secretion
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 95 kDa, 5.34
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2523 bp, 841 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[prfB]]'', ''[[yvyD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15547]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:secA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU35300

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/secA-prfB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10741]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP -> ADP + Pi + preprotein translocation

* '''Protein family:''' SecA family (according to Swiss-Prot)

* '''Paralogous protein(s):''' none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain

* '''Modification:'''

* '''Cofactor(s):''' magnesium

* '''Effectors of protein activity:''' anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans) [http://www.ncbi.nlm.nih.gov/pubmed/19924216 PubMed]

* '''Interactions:''' [[SecA]]-[[Ffh]], [[CsaA]]-[[SecA]], [[SecA]]-[[SecY]], [[SecA]]-[[SecA]]

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1TF5 1TF5] (open structure), [http://www.rcsb.org/pdb/explore.do?structureId=2IBM 2IBM]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P28366 P28366]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35300]

* '''E.C. number:'''

=== Additional information===

:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]

=Expression and regulation=

* '''Operon:''' ''[[secA]]-[[prfB]]''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]

=Biological materials =

* '''Mutant: ''Pspac-[[secA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/12218047 PubMed]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>19933328, 19924216, 18923516,12873133,12242434,1385592,10816431,19850053 ,9880811, </pubmed>

[[Category:Protein-coding genes]]

SecA

2009-12-14T10:16:11Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' preprotein translocase subunit (ATPase) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''secA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''div, div-341, ts-341 ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || preprotein translocase subunit (ATPase)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || protein secretion
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 95 kDa, 5.34
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2523 bp, 841 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[prfB]]'', ''[[yvyD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15547]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:secA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU35300

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/secA-prfB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10741]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP -> ADP + Pi + preprotein translocation

* '''Protein family:''' SecA family (according to Swiss-Prot)

* '''Paralogous protein(s):''' none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain

* '''Modification:'''

* '''Cofactor(s):''' magnesium

* '''Effectors of protein activity:''' anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans)

* '''Interactions:''' [[SecA]]-[[Ffh]], [[CsaA]]-[[SecA]], [[SecA]]-[[SecY]], [[SecA]]-[[SecA]]

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1TF5 1TF5] (open structure), [http://www.rcsb.org/pdb/explore.do?structureId=2IBM 2IBM]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P28366 P28366]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35300]

* '''E.C. number:'''

=== Additional information===

:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
=Expression and regulation=

* '''Operon:''' ''[[secA]]-[[prfB]]''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]

=Biological materials =

* '''Mutant: ''Pspac-[[secA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/12218047 PubMed]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>19933328, 19924216, 18923516,12873133,12242434,1385592,10816431,19850053 ,9880811, </pubmed>

[[Category:Protein-coding genes]]

SecA

2009-12-14T10:14:15Z

SHalbedel: /* References */

* '''Description:''' preprotein translocase subunit (ATPase) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''secA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''div, div-341, ts-341 ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || preprotein translocase subunit (ATPase)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || protein secretion
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 95 kDa, 5.34
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2523 bp, 841 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[prfB]]'', ''[[yvyD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15547]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:secA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU35300

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/secA-prfB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10741]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP -> ADP + Pi + preprotein translocation

* '''Protein family:''' SecA family (according to Swiss-Prot)

* '''Paralogous protein(s):''' none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain

* '''Modification:'''

* '''Cofactor(s):''' magnesium

* '''Effectors of protein activity:''' anionic phospholipids, preprotein, SecY

* '''Interactions:''' [[SecA]]-[[Ffh]], [[CsaA]]-[[SecA]], [[SecA]]-[[SecY]], [[SecA]]-[[SecA]]

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1TF5 1TF5] (open structure), [http://www.rcsb.org/pdb/explore.do?structureId=2IBM 2IBM]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P28366 P28366]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35300]

* '''E.C. number:'''

=== Additional information===

:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
=Expression and regulation=

* '''Operon:''' ''[[secA]]-[[prfB]]''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]

=Biological materials =

* '''Mutant: ''Pspac-[[secA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/12218047 PubMed]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>19933328, 19924216, 18923516,12873133,12242434,1385592,10816431,19850053 ,9880811, </pubmed>

[[Category:Protein-coding genes]]

AprE

2009-09-04T12:03:44Z

SHalbedel: /* Biological materials */

* '''Description:''' extracellular alkaline serine protease (subtilisin E) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''aprE''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sprE ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || extracellular alkaline serine protease (subtilisin E))
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || protein degradation
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 9.342
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfN]]'', ''[[yhfO]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12870]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:aprE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU10300

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/aprE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10190]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)

* '''Protein family:''' peptidase S8 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[AprE]]-[[PaiA]]

* '''Localization:''' secreted (according to Swiss-Prot), extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1SBC 1SBC]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P04189 P04189]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10300]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1898931 PubMed]
** repressed during exponential growth ([[ScoC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467 PubMed]
** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/2504584 PubMed]

* '''Regulatory mechanism:'''
** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1898931 PubMed]
** [[ScoC]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467 PubMed]
** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/2504584 PubMed]

* '''Additional information:''' the mRNA is extremely stable (more than 25 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/11101663 PubMed]

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

* '''Additional information:''' The pAPNC213 vector published by Morimoto et al. (2002) is an insertion plasmid for IPTG inducible constructs that can be integrated into the chromosomal aprE locus by replacing the aprE open reading frame via double homologous recombination. [http://www.ncbi.nlm.nih.gov/sites/entrez/12427945 PubMed]

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 19710937 3082852,2447063,2447062,19251843,18414485,6322190,12884008,15126467,1898931,1906467,2504584,18957862 11101663 12055299, </pubmed>

[[Category:Protein-coding genes]]

AprE

2009-09-04T12:02:53Z

SHalbedel: /* Biological materials */

* '''Description:''' extracellular alkaline serine protease (subtilisin E) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''aprE''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sprE ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || extracellular alkaline serine protease (subtilisin E))
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || protein degradation
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 9.342
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfN]]'', ''[[yhfO]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12870]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:aprE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU10300

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/aprE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10190]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)

* '''Protein family:''' peptidase S8 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[AprE]]-[[PaiA]]

* '''Localization:''' secreted (according to Swiss-Prot), extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1SBC 1SBC]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P04189 P04189]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10300]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1898931 PubMed]
** repressed during exponential growth ([[ScoC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467 PubMed]
** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/2504584 PubMed]

* '''Regulatory mechanism:'''
** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1898931 PubMed]
** [[ScoC]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467 PubMed]
** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/2504584 PubMed]

* '''Additional information:''' the mRNA is extremely stable (more than 25 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/11101663 PubMed]

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

* '''Additional information:''' The pAPNC213 vector published by Morimoto et al. (2002) is an insertion plasmid for IPTG inducible constructs that can be integrated into the chromosomal aprE locus by replacing the aprE open reading frame via double homologous recombination.

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 19710937 3082852,2447063,2447062,19251843,18414485,6322190,12884008,15126467,1898931,1906467,2504584,18957862 11101663 12055299, </pubmed>

[[Category:Protein-coding genes]]

MinJ

2009-09-02T10:39:27Z

SHalbedel: /* References */

* '''Description:''' topological determinant of cell division 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''minJ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvjD, swrAB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || topological determinant of cell division
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || cell division site selection
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 9.057
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1191 bp, 397 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvkA]]'', ''[[swrAA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15539]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yvjD_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU35220

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14111]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[MinJ]]-[[MinD]], [[MinJ]]-[[DivIVA]]

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34375 O34375]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35220]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Daniel Kearns]], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage]

=Your additional remarks=

=References=

<pubmed>19019154, 16030230,18976281,, </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T13:17:31Z

SHalbedel: /* Phenotypes of a mutant */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed] A divIVA mutant has a severe sporulation defect. [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed].

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T09:04:02Z

SHalbedel: /* References */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed]. A divIVA mutant has a severe sporulation defect [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed].

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed].

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T09:01:39Z

SHalbedel: /* Additional information */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed]. A divIVA mutant has a severe sporulation defect [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed].

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
Filamentation is suppressed by mutations in minCD [http://www.ncbi.nlm.nih.gov/sites/entrez/9045828 PubMed].

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed].

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T08:44:55Z

SHalbedel: /* Phenotypes of a mutant */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed]. A divIVA mutant has a severe sporulation defect [http://www.ncbi.nlm.nih.gov/sites/entrez/11445541 PubMed].

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
filamentation is suppressed by minCD mutations

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed].

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T08:41:20Z

SHalbedel: /* Expression and regulation */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
filamentation is suppressed by minCD mutations

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed].

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T08:37:47Z

SHalbedel: /* The protein */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
filamentation is suppressed by minCD mutations

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed] It also recruits RacA to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [http://www.ncbi.nlm.nih.gov/sites/entrez/18388125 PubMed].

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 [http://www.ncbi.nlm.nih.gov/sites/entrez/15985609 PubMed].

* '''Cofactor(s):''' not known

* '''Effectors of protein activity:''' not known

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [http://www.ncbi.nlm.nih.gov/sites/entrez/9219999 PubMed].

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T08:30:47Z

SHalbedel: /* Additional information */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===
filamentation is suppressed by minCD mutations

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. It also recruits RacA to the distal pole of the prespore.

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain.

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73.

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles.

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T08:29:16Z

SHalbedel: /* Biological materials */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. It also recruits RacA to the distal pole of the prespore.

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain.

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73.

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles.

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T08:28:52Z

SHalbedel: /* Biological materials */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. It also recruits RacA to the distal pole of the prespore.

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain.

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73.

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles.

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

DivIVA

2009-08-27T08:27:20Z

SHalbedel: /* References */

* '''Description:''' cell-division initiation protein (septum placement) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''divIVA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division initiation protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 4.846
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 492 bp, 164 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmH]]'', ''[[ileS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13416]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:divIVA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15420

===Phenotypes of a mutant ===
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11835]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' DivIVA is required for polar localisation of MinCD via MinJ. It also recruits RacA to the distal pole of the prespore.

* '''Protein family:''' gpsB family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[GpsB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' The first 60 amino acids constitute a lipid binding domain. [http://www.ncbi.nlm.nih.gov/sites/entrez/19478798 PubMed]
Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain.

* '''Modification:''' The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73.

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]], [[DivIVA]]-[[DivIVA]]

* '''Localization:''' DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles.

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71021 P71021]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' one gene cistron

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' divIVA::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Leendert Hamoen]], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen]

[[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]

=Your additional remarks=

=References=

<pubmed>19654604,19666580,9219999,19019154,12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999 </pubmed>

[[Category:Protein-coding genes]]

Sven Halbedel

2009-08-27T08:19:04Z

SHalbedel:

==Sven Halbedel==
*Institution: Robert Koch Institute
*Position: Scientist
*Contact: halbedels@rki.de [mailto:halbedels@rki.de send mail]

==Research Interests==
*Protein targeting to, in and across membranes
*Chaperone activities in secretion systems
*Spatiotemporal coordination of bacterial cell division
*DivIVA like proteins
*Minimal genomes
*Listeria virulence

==Looking for==
*students interested in molecular microbiology internships

GpsB

2009-07-16T08:50:47Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' required for removal of PBP1 from the cell pole after completion of cell pole maturation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gpsB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypsB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell division protein, member of the divisome
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || cell division, cell elongation
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 5.629
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 294 bp, 98 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnpB]]'', ''[[ypsA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14135]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ypsB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU22180

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11454]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[DivIVA]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' contains a DivIVA-like N-terminal domain

* '''Modification:''' phosphorylation on (Ser-73 OR Thr-75) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''dynamic, shuttles between lateral membrane and the divison septum [http://www.ncbi.nlm.nih.gov/sites/entrez/18363795 PubMed]

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P50839 P50839]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22180]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>18363795,18776011,, 19429628, 17218307, 18363795 </pubmed>

GpsB

2009-07-16T08:48:01Z

SHalbedel: /* Extended information on the protein */

* '''Description:''' required for removal of PBP1 from the cell pole after completion of cell pole maturation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gpsB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypsB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell division protein, member of the divisome
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || cell division, cell elongation
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 5.629
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 294 bp, 98 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnpB]]'', ''[[ypsA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14135]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ypsB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU22180

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11454]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[DivIVA]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' contains a DivIVA-like N-terminal domain

* '''Modification:''' phosphorylation on (Ser-73 OR Thr-75) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P50839 P50839]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22180]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>18363795,18776011,, 19429628, 17218307, 18363795 </pubmed>