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		<id>http://subtiwiki-test.uni-goettingen.de/wiki//api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Raphael2215</id>
		<title>SubtiWiki - User contributions [en]</title>
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		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Special:Contributions/Raphael2215"/>
		<updated>2026-04-07T12:54:54Z</updated>
		<subtitle>User contributions</subtitle>
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	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=MciZ&amp;diff=208935</id>
		<title>MciZ</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=MciZ&amp;diff=208935"/>
				<updated>2015-12-03T07:36:21Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: mciZ}}&lt;br /&gt;
&lt;br /&gt;
* '''Description:''' [[FtsZ]] inhibition peptide &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''mciZ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || '' ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[FtsZ]] inhibition peptide&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || inhibits GTPase activity of [[FtsZ]] and Z ring formation during sporulation&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=3375 mciZ]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MciZ MciZ]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 4.8 kDa, y.yy &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 120 bp, 40 aa&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[nudF]]'', ''[[yqkF]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt     Gene sequence      (+200bp)   ]''' &lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_protein.txt Protein sequence]'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:nudF_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell division]]}},&lt;br /&gt;
{{SubtiWiki category|[[short peptides]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:'''&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
This gene was only recently identified. [http://www.ncbi.nlm.nih.gov/sites/entrez/18284588 PubMed]&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' inhibits GTPase activity of [[FtsZ]] and Z ring formation during sporulation {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:''' &lt;br /&gt;
** [[MciZ]]-[[FtsZ]] {{PubMed|18284588}}, K&amp;lt;sub&amp;gt;D&amp;lt;/sub&amp;gt;: 150 nM {{PubMed|25848052}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''	&lt;br /&gt;
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=2MRW 2MRW] {{PubMed|25848052}}&lt;br /&gt;
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4u39 4U39] ([[FtsZ]]-[[MciZ]] complex) {{PubMed|25848052}}&lt;br /&gt;
&lt;br /&gt;
* '''Swiss prot entry:'''&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:'''&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' &lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=3375 mciZ] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
	&lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 19680248 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
==Original Publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;,18284588, 25848052&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:short peptide]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YxjC&amp;diff=208826</id>
		<title>YxjC</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YxjC&amp;diff=208826"/>
				<updated>2015-09-21T12:16:25Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: yxjC}}&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=HbuT&amp;diff=208825</id>
		<title>HbuT</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=HbuT&amp;diff=208825"/>
				<updated>2015-09-21T12:09:55Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: Created page with &amp;quot;{{#bank: hbuT}}&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: hbuT}}&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YxjC&amp;diff=208824</id>
		<title>YxjC</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YxjC&amp;diff=208824"/>
				<updated>2015-09-21T12:08:50Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: hbuT}}&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Utilization_of_specific_carbon_sources&amp;diff=208823</id>
		<title>Utilization of specific carbon sources</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Utilization_of_specific_carbon_sources&amp;diff=208823"/>
				<updated>2015-09-21T12:07:36Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{CategoryTree&lt;br /&gt;
|Parents=&lt;br /&gt;
* 2. [[Metabolism]]&lt;br /&gt;
** 2.2. [[Carbon metabolism]]&lt;br /&gt;
|Neighbours=&lt;br /&gt;
* 2.2.1. [[Carbon core metabolism]]&lt;br /&gt;
* 2.2.2. [[Utilization of specific carbon sources]]&lt;br /&gt;
|Related=&lt;br /&gt;
* [[CcpA regulon]]&lt;br /&gt;
|}}&lt;br /&gt;
&lt;br /&gt;
== Genes in this functional category ==&lt;br /&gt;
&lt;br /&gt;
=== Utilization of organic acids === &lt;br /&gt;
* ''[[acsA]]''&lt;br /&gt;
* ''[[acuA]]''&lt;br /&gt;
* ''[[acuC]]''&lt;br /&gt;
* ''[[cimH]]''&lt;br /&gt;
* ''[[citH]]''&lt;br /&gt;
* ''[[citM]]''&lt;br /&gt;
* ''[[citS]]''&lt;br /&gt;
* ''[[citT]]''&lt;br /&gt;
* ''[[dctB]]''&lt;br /&gt;
* ''[[dctP]]''&lt;br /&gt;
* ''[[dctR]]''&lt;br /&gt;
* ''[[dctS]]''&lt;br /&gt;
* ''[[hbuT]]''&lt;br /&gt;
* ''[[lutA]]''&lt;br /&gt;
* ''[[lutB]]''&lt;br /&gt;
* ''[[lutC]]''&lt;br /&gt;
* ''[[lutP]]''&lt;br /&gt;
* ''[[lutR]]''&lt;br /&gt;
* ''[[maeA]]''&lt;br /&gt;
* ''[[maeN]]''&lt;br /&gt;
* ''[[malK]]''&lt;br /&gt;
* ''[[malR]]''&lt;br /&gt;
* ''[[malS]]''&lt;br /&gt;
* ''[[mleA]]''&lt;br /&gt;
* ''[[mleN]]''&lt;br /&gt;
* ''[[scoA]]''&lt;br /&gt;
* ''[[scoB]]''&lt;br /&gt;
* ''[[yflS]]''&lt;br /&gt;
* ''[[yoaB]]''&lt;br /&gt;
* ''[[yraO]]''&lt;br /&gt;
* ''[[ytsJ]]''&lt;br /&gt;
* ''[[yxjF]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of acetoin === &lt;br /&gt;
* ''[[acoA]]''&lt;br /&gt;
* ''[[acoB]]''&lt;br /&gt;
* ''[[acoC]]''&lt;br /&gt;
* ''[[acoL]]''&lt;br /&gt;
* ''[[acoR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of glycerol/ glycerol-3-phosphate === &lt;br /&gt;
* ''[[glpD]]''&lt;br /&gt;
* ''[[glpF]]''&lt;br /&gt;
* ''[[glpK]]''&lt;br /&gt;
* ''[[glpP]]''&lt;br /&gt;
* ''[[glpQ]]''&lt;br /&gt;
* ''[[glpT]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of ribose === &lt;br /&gt;
* ''[[rbsA]]''&lt;br /&gt;
* ''[[rbsB]]''&lt;br /&gt;
* ''[[rbsC]]''&lt;br /&gt;
* ''[[rbsD]]''&lt;br /&gt;
* ''[[rbsK]]''&lt;br /&gt;
* ''[[rbsR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of xylan/ xylose === &lt;br /&gt;
* ''[[araE]]''&lt;br /&gt;
* ''[[xylA]]''&lt;br /&gt;
* ''[[xylB]]''&lt;br /&gt;
* ''[[xylR]]''&lt;br /&gt;
* ''[[xynA]]''&lt;br /&gt;
* ''[[xynB]]''&lt;br /&gt;
* ''[[xynP]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of arabinan/ arabinose/ arabitol === &lt;br /&gt;
* ''[[abf2]]''&lt;br /&gt;
* ''[[abfA]]''&lt;br /&gt;
* ''[[abn2]]''&lt;br /&gt;
* ''[[abnA]]''&lt;br /&gt;
*'' [[araA]]''&lt;br /&gt;
* ''[[araB]]''&lt;br /&gt;
* ''[[araD]]''&lt;br /&gt;
* ''[[araE]]''&lt;br /&gt;
* ''[[araN]]''&lt;br /&gt;
* ''[[araP]]''&lt;br /&gt;
* ''[[araQ]]''&lt;br /&gt;
* ''[[araR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of fructose === &lt;br /&gt;
* ''[[fruA]]''&lt;br /&gt;
* ''[[fruK]]''&lt;br /&gt;
* ''[[fruR]]''&lt;br /&gt;
* ''[[levD]]''&lt;br /&gt;
* ''[[levE]]''&lt;br /&gt;
* ''[[levF]]''&lt;br /&gt;
* ''[[levG]]''&lt;br /&gt;
* ''[[levR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of galactose === &lt;br /&gt;
* ''[[araE]]''&lt;br /&gt;
* ''[[galE]]''&lt;br /&gt;
* ''[[galK]]''&lt;br /&gt;
* ''[[galM]]''&lt;br /&gt;
* ''[[galT]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of mannose === &lt;br /&gt;
* ''[[manA]]''&lt;br /&gt;
* ''[[manP]]''&lt;br /&gt;
* ''[[manR]]''&lt;br /&gt;
* ''[[pmi]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of mannitol === &lt;br /&gt;
* ''[[mtlA]]''&lt;br /&gt;
* ''[[mtlD]]''&lt;br /&gt;
* ''[[mtlF]]''&lt;br /&gt;
* ''[[mtlR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of glucitol === &lt;br /&gt;
* ''[[gutB]]''&lt;br /&gt;
* ''[[gutP]]''&lt;br /&gt;
* ''[[gutR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of gluconate === &lt;br /&gt;
* ''[[gntK]]''&lt;br /&gt;
* ''[[gntP]]''&lt;br /&gt;
* ''[[gntR]]''&lt;br /&gt;
* ''[[gntZ]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of glucarate/galactarate === &lt;br /&gt;
* ''[[ycbC]]''&lt;br /&gt;
* ''[[ycbD]]''&lt;br /&gt;
* ''[[ycbE]]''&lt;br /&gt;
* ''[[ycbF]]''&lt;br /&gt;
* ''[[ycbG]]''&lt;br /&gt;
*'' [[ycbH]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of hexuronate  === &lt;br /&gt;
* ''[[exuR]]''&lt;br /&gt;
* ''[[exuT]]''&lt;br /&gt;
* ''[[kdgA]]''&lt;br /&gt;
* ''[[kdgK]]''&lt;br /&gt;
* ''[[kdgR]]''&lt;br /&gt;
* ''[[kdgT]]''&lt;br /&gt;
* ''[[kduD]]''&lt;br /&gt;
* ''[[kduI]]''&lt;br /&gt;
* ''[[uxaA]]''&lt;br /&gt;
* ''[[uxaB]]''&lt;br /&gt;
* ''[[uxaC]]''&lt;br /&gt;
* ''[[uxuA]]''&lt;br /&gt;
* ''[[yjmB]]''&lt;br /&gt;
* ''[[yjmC]]''&lt;br /&gt;
* ''[[yjmD]]''&lt;br /&gt;
* ''[[yjmF]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of inositol === &lt;br /&gt;
* ''[[iolA]]''&lt;br /&gt;
* ''[[iolB]]''&lt;br /&gt;
* ''[[iolC]]''&lt;br /&gt;
* ''[[iolD]]''&lt;br /&gt;
* ''[[iolE]]''&lt;br /&gt;
* ''[[iolF]]''&lt;br /&gt;
* ''[[iolG]]''&lt;br /&gt;
* ''[[iolH]]''&lt;br /&gt;
* ''[[iolI]]''&lt;br /&gt;
* ''[[iolJ]]''&lt;br /&gt;
* ''[[iolR]]''&lt;br /&gt;
* ''[[iolS]]''&lt;br /&gt;
* ''[[iolT]]''&lt;br /&gt;
* ''[[iolW]]''&lt;br /&gt;
* ''[[iolX]]''&lt;br /&gt;
* ''[[yfiG]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of amino sugars === &lt;br /&gt;
* ''[[frlB]]''&lt;br /&gt;
* ''[[frlD]]''&lt;br /&gt;
* ''[[frlM]]''&lt;br /&gt;
* ''[[frlN]]''&lt;br /&gt;
*'' [[frlO]]''&lt;br /&gt;
* ''[[frlR]]''&lt;br /&gt;
* ''[[gamA]]''&lt;br /&gt;
* ''[[gamP]]''&lt;br /&gt;
* ''[[gamR]]''&lt;br /&gt;
* ''[[murP]]''&lt;br /&gt;
* ''[[murQ]]''&lt;br /&gt;
* ''[[murR]]''&lt;br /&gt;
* ''[[nagA]]''&lt;br /&gt;
* ''[[nagB]]''&lt;br /&gt;
* ''[[nagP]]''&lt;br /&gt;
* ''[[nagR]]''&lt;br /&gt;
* ''[[yurJ]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of beta-glucosides === &lt;br /&gt;
* ''[[bglA]]''&lt;br /&gt;
* ''[[bglH]]''&lt;br /&gt;
* ''[[bglP]]''&lt;br /&gt;
* ''[[licT]]''&lt;br /&gt;
* ''[[yckE]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of sucrose === &lt;br /&gt;
* ''[[fruC]]''&lt;br /&gt;
* ''[[sacA]]''&lt;br /&gt;
* ''[[sacB]]''&lt;br /&gt;
* ''[[sacP]]''&lt;br /&gt;
* ''[[sacT]]''&lt;br /&gt;
* ''[[sacX]]''&lt;br /&gt;
* ''[[sacY]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of trehalose === &lt;br /&gt;
* ''[[glcK]]''&lt;br /&gt;
* ''[[treA]]''&lt;br /&gt;
* ''[[treP]]''&lt;br /&gt;
* ''[[treR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of melibiose === &lt;br /&gt;
* ''[[amyC]]''&lt;br /&gt;
* ''[[amyD]]''&lt;br /&gt;
* ''[[melA]]''&lt;br /&gt;
* ''[[msmE]]''&lt;br /&gt;
* ''[[msmR]]''&lt;br /&gt;
* ''[[msmX]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of maltose === &lt;br /&gt;
* ''[[glcK]]''&lt;br /&gt;
*'' [[glvR]]''&lt;br /&gt;
* ''[[malA]]''&lt;br /&gt;
* ''[[malP]]''&lt;br /&gt;
* ''[[yvdK]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of starch/ maltodextrin === &lt;br /&gt;
* ''[[amyE]]''&lt;br /&gt;
* ''[[amyX]]''&lt;br /&gt;
* ''[[glcK]]''&lt;br /&gt;
* ''[[maa]]''&lt;br /&gt;
* ''[[malL]]''&lt;br /&gt;
* ''[[mdxE]]''&lt;br /&gt;
* ''[[mdxF]]''&lt;br /&gt;
* ''[[mdxG]]''&lt;br /&gt;
* ''[[msmX]]''&lt;br /&gt;
* ''[[pgcM]]''&lt;br /&gt;
* ''[[yvdE]]''&lt;br /&gt;
* ''[[yvdF]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of glucomannan === &lt;br /&gt;
* ''[[gmuA]]''&lt;br /&gt;
* ''[[gmuB]]''&lt;br /&gt;
* ''[[gmuC]]''&lt;br /&gt;
* ''[[gmuD]]''&lt;br /&gt;
* ''[[gmuE]]''&lt;br /&gt;
* ''[[gmuF]]''&lt;br /&gt;
* ''[[gmuG]]''&lt;br /&gt;
* ''[[gmuR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of rhamnose and pectin === &lt;br /&gt;
* ''[[lplD]]''&lt;br /&gt;
* ''[[rhaA]]''&lt;br /&gt;
* ''[[rhaB]]''&lt;br /&gt;
* ''[[rhaEW]]''&lt;br /&gt;
* ''[[rhaM]]''&lt;br /&gt;
* ''[[rhaR]]''&lt;br /&gt;
* ''[[rhiF]]''&lt;br /&gt;
* ''[[rhiG]]''&lt;br /&gt;
* ''[[rhiL]]''&lt;br /&gt;
* ''[[rhiN]]''&lt;br /&gt;
* ''[[rhgR]]''&lt;br /&gt;
* ''[[ybcM]]''&lt;br /&gt;
* ''[[yesT]]''&lt;br /&gt;
* ''[[yesV]]''&lt;br /&gt;
* ''[[yesW]]''&lt;br /&gt;
* ''[[yesX]]''&lt;br /&gt;
* ''[[yesY]]''&lt;br /&gt;
* ''[[yesZ]]''&lt;br /&gt;
* ''[[yteR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of galactan === &lt;br /&gt;
* ''[[cycB]]''&lt;br /&gt;
* ''[[ganA]]''&lt;br /&gt;
* ''[[ganB]]''&lt;br /&gt;
*'' [[ganP]]''&lt;br /&gt;
* ''[[ganQ]]''&lt;br /&gt;
* ''[[lacR]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of other polymeric carbohydrates === &lt;br /&gt;
* ''[[bglC]]''&lt;br /&gt;
* ''[[bglS]]''&lt;br /&gt;
* ''[[csn]]''&lt;br /&gt;
* ''[[levB]]''&lt;br /&gt;
* ''[[levD]]''&lt;br /&gt;
* ''[[levE]]''&lt;br /&gt;
* ''[[levF]]''&lt;br /&gt;
* ''[[levG]]''&lt;br /&gt;
* ''[[levR]]''&lt;br /&gt;
* ''[[licA]]''&lt;br /&gt;
* ''[[licB]]''&lt;br /&gt;
* ''[[licC]]''&lt;br /&gt;
* ''[[licH]]''&lt;br /&gt;
* ''[[licR]]''&lt;br /&gt;
* ''[[licT]]''&lt;br /&gt;
* ''[[pel]]''&lt;br /&gt;
* ''[[pelB]]''&lt;br /&gt;
* ''[[pelC]]''&lt;br /&gt;
* ''[[sacC]]''&lt;br /&gt;
* ''[[senS]]''&lt;br /&gt;
* ''[[xynC]]''&lt;br /&gt;
* ''[[xynD]]''&lt;br /&gt;
* ''[[ysdC]]''&lt;br /&gt;
* ''[[ytoP]]''&lt;br /&gt;
* ''[[yugT]]''&lt;br /&gt;
&lt;br /&gt;
=== Utilization of other pentoses and hexoses===&lt;br /&gt;
* ''[[ydaE]]''&lt;br /&gt;
&lt;br /&gt;
== Utilization of specific carbon sources in [http://subtiwiki.uni-goettingen.de/subtipathways.html ''Subti''Pathways]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Utilization of different carbon sources]==&lt;br /&gt;
&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 11018147 19202299 17982469 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
=Back to [[categories]]=&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Hbut&amp;diff=208822</id>
		<title>Hbut</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Hbut&amp;diff=208822"/>
				<updated>2015-09-21T12:07:16Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: Created page with &amp;quot;{{#bank: hbuT}}&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: hbuT}}&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208408</id>
		<title>GudB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208408"/>
				<updated>2015-07-21T13:29:47Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: GudB}}&lt;br /&gt;
&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208407</id>
		<title>GudB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208407"/>
				<updated>2015-07-21T13:24:59Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase (cryptic in 168 and derivatives)&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''gudB''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''ypcA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || glutamate utilization, control of [[GltC]] activity&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960 gudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GudB GudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gudB gudB]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 47 kDa, 5.582  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1278 bp, 426 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[ypdA]]'', ''[[ypbH]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU22960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU22960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU22960 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:gudB_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:gudB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of amino acids]]}}, [[glutamate metabolism]], &lt;br /&gt;
{{SubtiWiki category|[[transcription factors and their control]]}},&lt;br /&gt;
{{SubtiWiki category|[[trigger enzyme]]}},&lt;br /&gt;
{{SubtiWiki category|[[phosphoproteins]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU22960&lt;br /&gt;
&lt;br /&gt;
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression]&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
* The gene is cryptic. If ''gudB'' is activated (''gudB1'' mutation), the bacteria are able to utilize glutamate as the only carbon source. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+18326565 PubMed]&lt;br /&gt;
* A ''[[rocG]] [[gudB]]'' mutant is sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin due to the downregulation of the [[SigW regulon]] {{PubMed|22178969}}&lt;br /&gt;
* transcription profile of a ''[[rocG]] [[gudB]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9 GEO] {{PubMed|22178969}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gudB.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11435]&lt;br /&gt;
&lt;br /&gt;
* '''GEO entry:''' [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9] (''[[rocG]] [[gudB]]'' mutant vs. wild type)&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamate + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O + NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; = 2-oxoglutarate + NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):''' [[RocG]]&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
** phosphorylated on Arg-56, Arg-83, and Arg-421 and/or Arg-423 {{PubMed|22517742}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;/NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[GltC]]-[[GudB]] {{PubMed|25711804}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K8Z 3K8Z] (enzymatically active GudB1) {{PubMed|20630473}} &lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P50735 P50735]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.2 1.4.1.2]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''gudB'' {{PubMed|22178973}}&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 gudB] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+9829940 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' constitutively expressed {{PubMed|22178973}} 	&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' GudB is subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 239 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 6565 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1157 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 580 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 888 {{PubMed|21395229}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
* '''Mutant:''' GP691 (''ΔgudB::cat''), GP1160 (''ΔgudB::aphA3'') both available in [[Jörg Stülke]]'s lab&lt;br /&gt;
* '''Mutant:''' BP442 (''ΔgudB::aphA3''), lacking the complete promoter, available in [[Fabian Commichau]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:''' &lt;br /&gt;
** for purification of GudB from ''E. coli'' carrying an N-terminal Strep-tag: pGP863 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for purification of GudB1 from ''E. coli'' carrying an N-terminal Strep-tag: pGP864 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for ectopic expression of ''gudB'' with its native promoter: pGP900 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** wild type ''gudB'', expression in ''B. subtilis'', in [[pBQ200]]: pGP1712, available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** pBP179 (N-terminal Strep-tag ''gudB+'', purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Jörg Stülke]]'s lab    &lt;br /&gt;
    &lt;br /&gt;
* '''lacZ fusion:''' pGP651 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''FLAG-tag construct:''' GP1194 (''gudB'', ''spc'', based on [[pGP1331]]), GP1195 (''gudB1'', ''spc'', based on [[pGP1331]]),  available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' (''gudB+'') B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
* '''Antibody:''' antibody against [[RocG]] recognizes GudB, available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]&lt;br /&gt;
* [[Fabian Commichau]] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
The GudB protein is active in other legacy ''B. subtilis'' strains (e.g. strain 122). Thus, it can be speculated that the ancestral ''gudB'' gene was not cryptic, but became so as a product of the &amp;quot;domestication&amp;quot; of ''B. subtilis'' 168 in the lab. [http://www.ncbi.nlm.nih.gov/pubmed/18723616 PubMed]&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19698086 8299344 7705101 19895831 22625175&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18603778,9829940 ,17183217 18723616, 18326565 20630473 17981983 21219666 22178973 22517742 23338837 22178969 23785476 24263382 24473333 25610436 25711804&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YwlE&amp;diff=208406</id>
		<title>YwlE</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YwlE&amp;diff=208406"/>
				<updated>2015-07-21T13:12:31Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: YwlE}}&lt;br /&gt;
&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208405</id>
		<title>GudB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208405"/>
				<updated>2015-07-21T13:10:09Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase (cryptic in 168 and derivatives)&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''gudB''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''ypcA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || glutamate utilization, control of [[GltC]] activity&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960 gudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GudB GudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gudB gudB]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 47 kDa, 5.582  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1278 bp, 426 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[ypdA]]'', ''[[ypbH]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU22960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU22960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU22960 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:gudB_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:gudB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of amino acids]]}}, [[glutamate metabolism]], &lt;br /&gt;
{{SubtiWiki category|[[transcription factors and their control]]}},&lt;br /&gt;
{{SubtiWiki category|[[trigger enzyme]]}},&lt;br /&gt;
{{SubtiWiki category|[[phosphoproteins]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU22960&lt;br /&gt;
&lt;br /&gt;
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression]&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
* The gene is cryptic. If ''gudB'' is activated (''gudB1'' mutation), the bacteria are able to utilize glutamate as the only carbon source. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+18326565 PubMed]&lt;br /&gt;
* A ''[[rocG]] [[gudB]]'' mutant is sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin due to the downregulation of the [[SigW regulon]] {{PubMed|22178969}}&lt;br /&gt;
* transcription profile of a ''[[rocG]] [[gudB]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9 GEO] {{PubMed|22178969}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gudB.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11435]&lt;br /&gt;
&lt;br /&gt;
* '''GEO entry:''' [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9] (''[[rocG]] [[gudB]]'' mutant vs. wild type)&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamate + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O + NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; = 2-oxoglutarate + NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):''' [[RocG]]&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
** phosphorylated on Arg-56, Arg-83, and Arg-421 and/or Arg-423 {{PubMed|22517742}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;/NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[GltC]]-[[GudB]] {{PubMed|25711804}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K8Z 3K8Z] (enzymatically active GudB1) {{PubMed|20630473}} &lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P50735 P50735]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.2 1.4.1.2]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''gudB'' {{PubMed|22178973}}&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 gudB] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+9829940 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' constitutively expressed {{PubMed|22178973}} 	&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' GudB is subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 239 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 6565 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1157 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 580 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 888 {{PubMed|21395229}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
* '''Mutant:''' GP691 (''ΔgudB::cat''), GP1160 (''ΔgudB::aphA3'') both available in [[Jörg Stülke]]'s lab&lt;br /&gt;
* '''Mutant:''' BP442 (''ΔgudB::aphA3''), lacking the complete promoter, available in [[Fabian Commichau]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:''' &lt;br /&gt;
** for purification of GudB from ''E. coli'' carrying an N-terminal Strep-tag: pGP863 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for purification of GudB1 from ''E. coli'' carrying an N-terminal Strep-tag: pGP864 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for ectopic expression of ''gudB'' with its native promoter: pGP900 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** wild type ''gudB'', expression in ''B. subtilis'', in [[pBQ200]]: pGP1712, available in [[Jörg Stülke]]'s lab        &lt;br /&gt;
* '''lacZ fusion:''' pGP651 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''FLAG-tag construct:''' GP1194 (''gudB'', ''spc'', based on [[pGP1331]]), GP1195 (''gudB1'', ''spc'', based on [[pGP1331]]),  available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' (''gudB+'') B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
* '''Antibody:''' antibody against [[RocG]] recognizes GudB, available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]&lt;br /&gt;
* [[Fabian Commichau]] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
The GudB protein is active in other legacy ''B. subtilis'' strains (e.g. strain 122). Thus, it can be speculated that the ancestral ''gudB'' gene was not cryptic, but became so as a product of the &amp;quot;domestication&amp;quot; of ''B. subtilis'' 168 in the lab. [http://www.ncbi.nlm.nih.gov/pubmed/18723616 PubMed]&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19698086 8299344 7705101 19895831 22625175&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18603778,9829940 ,17183217 18723616, 18326565 20630473 17981983 21219666 22178973 22517742 23338837 22178969 23785476 24263382 24473333 25610436 25711804&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=McsB&amp;diff=208404</id>
		<title>McsB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=McsB&amp;diff=208404"/>
				<updated>2015-07-21T13:09:09Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: McsB}}&lt;br /&gt;
&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=McsB&amp;diff=208403</id>
		<title>McsB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=McsB&amp;diff=208403"/>
				<updated>2015-07-21T13:08:51Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[protein kinases and phosphatases|protein arginine kinase]], adaptor protein, modulator of [[CtsR]]-dependent repression &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''mcsB''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''yacI ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[protein kinases and phosphatases|protein arginine kinase]]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || control of [[CtsR]] activity&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850 mcsB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=McsB McsB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mcsB mcsB]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 40 kDa, 5.068  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1089 bp, 363 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[mcsA]]'', ''[[clpC]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU00850 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU00850 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU00850 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:mcsB_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:mcsB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[protein modification]]}},&lt;br /&gt;
{{SubtiWiki category|[[transcription factors and their control]]}},&lt;br /&gt;
{{SubtiWiki category|[[sporulation proteins]]}},&lt;br /&gt;
{{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}},&lt;br /&gt;
{{SubtiWiki category|[[heat shock proteins]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[CtsR regulon]]}},&lt;br /&gt;
{{SubtiWiki regulon|[[SigB regulon]]}},&lt;br /&gt;
{{SubtiWiki regulon|[[SigF regulon]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU00850&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00850&amp;amp;redirect=T BSU00850]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10147]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' targets non-functional CtsR for degradation by [[ClpP]]/[[ClpC]] {{PubMed|20852588}}&lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' ATP:guanido phosphotransferase family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:''' &lt;br /&gt;
** autophosphorylation on specific arginine residues {{PubMed|20852588,21622759}}, dephosphorylation by [[YwlE]] {{PubMed|20852588,21622759}}&lt;br /&gt;
** autophosphorylation stimulates McsB activity as adaptor protein for [[ClpC]] {{PubMed|21622759}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:''' &lt;br /&gt;
** [[McsA]]-[[McsB]]-[[CtsR]],  [[McsB]]-[[CtsR]] {{PubMed|20852588}},   [[McsA]]-[[McsB]]&lt;br /&gt;
** [[McsB]]-[[YwlE]] {{PubMed|20852588}}&lt;br /&gt;
** [[McsB]]-[[ClpP]]/[[ClpC]] (rapid degradation of phosphorylated McsB)  {{PubMed|20852588}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00850&amp;amp;redirect=T BSU00850]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''	&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37570 P37570]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00850]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:'''&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ctsR]]-[[mcsA]]-[[mcsB]]-[[clpC]]-[[radA]]-[[disA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 mcsB] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2], [[SigF]] {{PubMed|16497325}}&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** expressed early during sporulation in the forespore ([[SigF]]) {{PubMed|16497325}}&lt;br /&gt;
** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]&lt;br /&gt;
** induced by heat ([[CtsR]]) [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
** [[CtsR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9987115 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:'''&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 142 {{PubMed|24696501}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:''' &lt;br /&gt;
** ''mcsB::aphA3'' availbale from the Gerth lab&lt;br /&gt;
** mcsBC167S::spec available from the Gerth lab&lt;br /&gt;
** GP1457 (''mcsB''::''aphA3''),  available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** BP69 (spc), available in [[Fabian Commichau]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab&lt;br /&gt;
** expression of native ''mcsB'' in ''B. subtilis'': pBP529186 (in [[pBQ200]]), available in [[Fabian Commichau]]'s lab&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:''' available in Gerth lab&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
* [[Ulf Gerth]], Greifswald, Germany&lt;br /&gt;
&lt;br /&gt;
* [[Fabian Commichau]] Göttingen, Germany&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&lt;br /&gt;
==Reviews==&lt;br /&gt;
&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 23375660 19609260&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
==Original Publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24825175 24263382 25610436&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208401</id>
		<title>GudB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208401"/>
				<updated>2015-07-21T13:01:47Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase (cryptic in 168 and derivatives)&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''gudB''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''ypcA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || glutamate utilization, control of [[GltC]] activity&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960 gudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GudB GudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gudB gudB]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 47 kDa, 5.582  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1278 bp, 426 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[ypdA]]'', ''[[ypbH]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU22960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU22960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU22960 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:gudB_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:gudB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of amino acids]]}}, [[glutamate metabolism]], &lt;br /&gt;
{{SubtiWiki category|[[transcription factors and their control]]}},&lt;br /&gt;
{{SubtiWiki category|[[trigger enzyme]]}},&lt;br /&gt;
{{SubtiWiki category|[[phosphoproteins]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU22960&lt;br /&gt;
&lt;br /&gt;
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression]&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
* The gene is cryptic. If ''gudB'' is activated (''gudB1'' mutation), the bacteria are able to utilize glutamate as the only carbon source. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+18326565 PubMed]&lt;br /&gt;
* A ''[[rocG]] [[gudB]]'' mutant is sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin due to the downregulation of the [[SigW regulon]] {{PubMed|22178969}}&lt;br /&gt;
* transcription profile of a ''[[rocG]] [[gudB]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9 GEO] {{PubMed|22178969}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gudB.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11435]&lt;br /&gt;
&lt;br /&gt;
* '''GEO entry:''' [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9] (''[[rocG]] [[gudB]]'' mutant vs. wild type)&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamate + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O + NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; = 2-oxoglutarate + NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):''' [[RocG]]&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
** phosphorylated on Arg-56, Arg-83, and Arg-421 and/or Arg-423 {{PubMed|22517742}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;/NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[GltC]]-[[GudB]] {{PubMed|25711804}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K8Z 3K8Z] (enzymatically active GudB1) {{PubMed|20630473}} &lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P50735 P50735]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.2 1.4.1.2]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''gudB'' {{PubMed|22178973}}&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 gudB] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+9829940 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' constitutively expressed {{PubMed|22178973}} 	&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' GudB is subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 239 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 6565 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1157 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 580 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 888 {{PubMed|21395229}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
* '''Mutant:''' GP691 (''ΔgudB::cat''), GP1160 (''ΔgudB::aphA3'') both available in [[Jörg Stülke]]'s lab&lt;br /&gt;
* '''Mutant:''' BP442 (''ΔgudB::aphA3''), lacking the complete promoter, available in [[Fabian Commichau]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:''' &lt;br /&gt;
** for purification of GudB from ''E. coli'' carrying an N-terminal Strep-tag: pGP863 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for purification of GudB1 from ''E. coli'' carrying an N-terminal Strep-tag: pGP864 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for ectopic expression of ''gudB'' with its native promoter: pGP900 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** wild type ''gudB'', expression in ''B. subtilis'', in [[pBQ200]]: pGP1712, available in [[Jörg Stülke]]'s lab        &lt;br /&gt;
* '''lacZ fusion:''' pGP651 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''FLAG-tag construct:''' GP1194 (''gudB'', ''spc'', based on [[pGP1331]]), GP1195 (''gudB1'', ''spc'', based on [[pGP1331]]),  available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' (''gudB+'') B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
* '''Antibody:''' antibody against [[RocG]] recognizes GudB, available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]&lt;br /&gt;
* [[Fabian Commichau]] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
The GudB protein is active in other legacy ''B. subtilis'' strains (e.g. strain 122). Thus, it can be speculated that the ancestral ''gudB'' gene was not cryptic, but became so as a product of the &amp;quot;domestication&amp;quot; of ''B. subtilis'' 168 in the lab. [http://www.ncbi.nlm.nih.gov/pubmed/18723616 PubMed]&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19698086 8299344 7705101 19895831 22625175&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18603778,9829940 ,17183217 18723616, 18326565 20630473 17981983 21219666 22178973 22517742 23338837 22178969 23785476 24263382 24473333 25610436 25711804&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208400</id>
		<title>GudB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208400"/>
				<updated>2015-07-21T13:01:17Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase (cryptic in 168 and derivatives)&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''gudB''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''ypcA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || glutamate utilization, control of [[GltC]] activity&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960 gudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GudB GudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gudB gudB]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 47 kDa, 5.582  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1278 bp, 426 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[ypdA]]'', ''[[ypbH]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU22960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU22960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU22960 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:gudB_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:gudB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of amino acids]]}}, [[glutamate metabolism]], &lt;br /&gt;
{{SubtiWiki category|[[transcription factors and their control]]}},&lt;br /&gt;
{{SubtiWiki category|[[trigger enzyme]]}},&lt;br /&gt;
{{SubtiWiki category|[[phosphoproteins]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU22960&lt;br /&gt;
&lt;br /&gt;
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression]&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
* The gene is cryptic. If ''gudB'' is activated (''gudB1'' mutation), the bacteria are able to utilize glutamate as the only carbon source. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+18326565 PubMed]&lt;br /&gt;
* A ''[[rocG]] [[gudB]]'' mutant is sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin due to the downregulation of the [[SigW regulon]] {{PubMed|22178969}}&lt;br /&gt;
* transcription profile of a ''[[rocG]] [[gudB]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9 GEO] {{PubMed|22178969}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gudB.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11435]&lt;br /&gt;
&lt;br /&gt;
* '''GEO entry:''' [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9] (''[[rocG]] [[gudB]]'' mutant vs. wild type)&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamate + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O + NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; = 2-oxoglutarate + NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):''' [[RocG]]&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
** phosphorylated on Arg-56, Arg-83, and Arg-421 and/or Arg-423 {{PubMed|22517742}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;/NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[GltC]]-[[GudB]] {{PubMed|25711804}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K8Z 3K8Z] (enzymatically active GudB1) {{PubMed|20630473}} &lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P50735 P50735]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.2 1.4.1.2]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''gudB'' {{PubMed|22178973}}&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 gudB] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+9829940 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' constitutively expressed {{PubMed|22178973}} 	&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' GudB is subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 239 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 6565 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1157 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 580 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 888 {{PubMed|21395229}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
* '''Mutant:''' GP691 (''ΔgudB::cat''), GP1160 (''ΔgudB::aphA3'') both available in [[Jörg Stülke]]'s lab&lt;br /&gt;
* '''Mutant:''' BP442 (''ΔgudB::aphA3''), lacking the complete promoter, available in [[Fabian Commichau]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:''' &lt;br /&gt;
** for purification of GudB from ''E. coli'' carrying an N-terminal Strep-tag: pGP863 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for purification of GudB1 from ''E. coli'' carrying an N-terminal Strep-tag: pGP864 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for ectopic expression of ''gudB'' with its native promoter: pGP900 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** wild type ''gudB'', expression in ''B. subtilis'', in [[pBQ200]]: pGP1712, available in [[Jörg Stülke]]'s lab        &lt;br /&gt;
* '''lacZ fusion:''' pGP651 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''FLAG-tag construct:''' GP1194 (''gudB'', ''spc'', based on [[pGP1331]]), GP1195 (''gudB1'', ''spc'', based on [[pGP1331]]),  available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' (''gudB'') B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Fabian Commichau]]'s lab &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
* '''Antibody:''' antibody against [[RocG]] recognizes GudB, available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]&lt;br /&gt;
* [[Fabian Commichau]] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
The GudB protein is active in other legacy ''B. subtilis'' strains (e.g. strain 122). Thus, it can be speculated that the ancestral ''gudB'' gene was not cryptic, but became so as a product of the &amp;quot;domestication&amp;quot; of ''B. subtilis'' 168 in the lab. [http://www.ncbi.nlm.nih.gov/pubmed/18723616 PubMed]&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19698086 8299344 7705101 19895831 22625175&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18603778,9829940 ,17183217 18723616, 18326565 20630473 17981983 21219666 22178973 22517742 23338837 22178969 23785476 24263382 24473333 25610436 25711804&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208399</id>
		<title>GudB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208399"/>
				<updated>2015-07-21T12:59:06Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Biological materials */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase (cryptic in 168 and derivatives)&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''gudB''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''ypcA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || glutamate utilization, control of [[GltC]] activity&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960 gudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GudB GudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gudB gudB]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 47 kDa, 5.582  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1278 bp, 426 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[ypdA]]'', ''[[ypbH]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU22960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU22960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU22960 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:gudB_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:gudB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of amino acids]]}}, [[glutamate metabolism]], &lt;br /&gt;
{{SubtiWiki category|[[transcription factors and their control]]}},&lt;br /&gt;
{{SubtiWiki category|[[trigger enzyme]]}},&lt;br /&gt;
{{SubtiWiki category|[[phosphoproteins]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU22960&lt;br /&gt;
&lt;br /&gt;
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression]&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
* The gene is cryptic. If ''gudB'' is activated (''gudB1'' mutation), the bacteria are able to utilize glutamate as the only carbon source. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+18326565 PubMed]&lt;br /&gt;
* A ''[[rocG]] [[gudB]]'' mutant is sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin due to the downregulation of the [[SigW regulon]] {{PubMed|22178969}}&lt;br /&gt;
* transcription profile of a ''[[rocG]] [[gudB]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9 GEO] {{PubMed|22178969}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gudB.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11435]&lt;br /&gt;
&lt;br /&gt;
* '''GEO entry:''' [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9] (''[[rocG]] [[gudB]]'' mutant vs. wild type)&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamate + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O + NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; = 2-oxoglutarate + NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):''' [[RocG]]&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
** phosphorylated on Arg-56, Arg-83, and Arg-421 and/or Arg-423 {{PubMed|22517742}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;/NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[GltC]]-[[GudB]] {{PubMed|25711804}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K8Z 3K8Z] (enzymatically active GudB1) {{PubMed|20630473}} &lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P50735 P50735]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.2 1.4.1.2]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''gudB'' {{PubMed|22178973}}&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 gudB] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+9829940 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' constitutively expressed {{PubMed|22178973}} 	&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' GudB is subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 239 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 6565 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1157 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 580 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 888 {{PubMed|21395229}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
* '''Mutant:''' GP691 (''ΔgudB::cat''), GP1160 (''ΔgudB::aphA3'') both available in [[Jörg Stülke]]'s lab&lt;br /&gt;
* '''Mutant:''' BP442 (''ΔgudB::aphA3''), lacking the complete promoter, available in [[Fabian Commichau]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:''' &lt;br /&gt;
** for purification of GudB from ''E. coli'' carrying an N-terminal Strep-tag: pGP863 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for purification of GudB1 from ''E. coli'' carrying an N-terminal Strep-tag: pGP864 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for ectopic expression of ''gudB'' with its native promoter: pGP900 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** wild type ''gudB'', expression in ''B. subtilis'', in [[pBQ200]]: pGP1712, available in [[Jörg Stülke]]'s lab        &lt;br /&gt;
* '''lacZ fusion:''' pGP651 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''FLAG-tag construct:''' GP1194 (''gudB'', ''spc'', based on [[pGP1331]]), GP1195 (''gudB1'', ''spc'', based on [[pGP1331]]),  available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab &lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' gudB+ gene, B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:''' antibody against [[RocG]] recognizes GudB, available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]&lt;br /&gt;
* [[Fabian Commichau]] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
The GudB protein is active in other legacy ''B. subtilis'' strains (e.g. strain 122). Thus, it can be speculated that the ancestral ''gudB'' gene was not cryptic, but became so as a product of the &amp;quot;domestication&amp;quot; of ''B. subtilis'' 168 in the lab. [http://www.ncbi.nlm.nih.gov/pubmed/18723616 PubMed]&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19698086 8299344 7705101 19895831 22625175&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18603778,9829940 ,17183217 18723616, 18326565 20630473 17981983 21219666 22178973 22517742 23338837 22178969 23785476 24263382 24473333 25610436 25711804&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208398</id>
		<title>GudB</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=GudB&amp;diff=208398"/>
				<updated>2015-07-21T12:57:20Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase (cryptic in 168 and derivatives)&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''gudB''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''ypcA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || glutamate utilization, control of [[GltC]] activity&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960 gudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GudB GudB]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gudB gudB]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 47 kDa, 5.582  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1278 bp, 426 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[ypdA]]'', ''[[ypbH]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU22960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU22960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU22960 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:gudB_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:gudB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22960]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of amino acids]]}}, [[glutamate metabolism]], &lt;br /&gt;
{{SubtiWiki category|[[transcription factors and their control]]}},&lt;br /&gt;
{{SubtiWiki category|[[trigger enzyme]]}},&lt;br /&gt;
{{SubtiWiki category|[[phosphoproteins]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU22960&lt;br /&gt;
&lt;br /&gt;
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 Expression]&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
* The gene is cryptic. If ''gudB'' is activated (''gudB1'' mutation), the bacteria are able to utilize glutamate as the only carbon source. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+18326565 PubMed]&lt;br /&gt;
* A ''[[rocG]] [[gudB]]'' mutant is sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin due to the downregulation of the [[SigW regulon]] {{PubMed|22178969}}&lt;br /&gt;
* transcription profile of a ''[[rocG]] [[gudB]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9 GEO] {{PubMed|22178969}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gudB.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11435]&lt;br /&gt;
&lt;br /&gt;
* '''GEO entry:''' [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&amp;amp;submit.x=22&amp;amp;submit.y=9] (''[[rocG]] [[gudB]]'' mutant vs. wild type)&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamate + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O + NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; = 2-oxoglutarate + NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):''' [[RocG]]&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
** phosphorylated on Arg-56, Arg-83, and Arg-421 and/or Arg-423 {{PubMed|22517742}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' NAD&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;/NADH + H&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[GltC]]-[[GudB]] {{PubMed|25711804}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU22960&amp;amp;redirect=T BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K8Z 3K8Z] (enzymatically active GudB1) {{PubMed|20630473}} &lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P50735 P50735]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22960]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.2 1.4.1.2]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''gudB'' {{PubMed|22178973}}&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudB_2402067_2403350_-1 gudB] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+9829940 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' constitutively expressed {{PubMed|22178973}} 	&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' GudB is subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 239 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 6565 {{PubMed|24696501}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1157 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 580 {{PubMed|21395229}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 888 {{PubMed|21395229}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
* '''Mutant:''' GP691 (''ΔgudB::cat''), GP1160 (''ΔgudB::aphA3'') both available in [[Jörg Stülke]]'s lab&lt;br /&gt;
* '''Mutant:''' BP442 (''ΔgudB::aphA3''), lacking the complete promoter, available in [[Fabian Commichau]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:''' &lt;br /&gt;
** for purification of GudB from ''E. coli'' carrying an N-terminal Strep-tag: pGP863 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for purification of GudB1 from ''E. coli'' carrying an N-terminal Strep-tag: pGP864 (in [[pGP172]]) available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** for ectopic expression of ''gudB'' with its native promoter: pGP900 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
** wild type ''gudB'', expression in ''B. subtilis'', in [[pBQ200]]: pGP1712, available in [[Jörg Stülke]]'s lab        &lt;br /&gt;
* '''lacZ fusion:''' pGP651 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''FLAG-tag construct:''' GP1194 (''gudB'', ''spc'', based on [[pGP1331]]), GP1195 (''gudB1'', ''spc'', based on [[pGP1331]]),  available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:''' antibody against [[RocG]] recognizes GudB, available in [[Jörg Stülke]]'s lab&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]&lt;br /&gt;
* [[Fabian Commichau]] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
The GudB protein is active in other legacy ''B. subtilis'' strains (e.g. strain 122). Thus, it can be speculated that the ancestral ''gudB'' gene was not cryptic, but became so as a product of the &amp;quot;domestication&amp;quot; of ''B. subtilis'' 168 in the lab. [http://www.ncbi.nlm.nih.gov/pubmed/18723616 PubMed]&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19698086 8299344 7705101 19895831 22625175&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18603778,9829940 ,17183217 18723616, 18326565 20630473 17981983 21219666 22178973 22517742 23338837 22178969 23785476 24263382 24473333 25610436 25711804&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YjzF&amp;diff=208395</id>
		<title>YjzF</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YjzF&amp;diff=208395"/>
				<updated>2015-07-21T08:28:59Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' unknown &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''yjzF''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || '' ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || -&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || unknown&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || unknown&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11928 yjzF]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 5.3 kDA, 11.749 &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 129 bp, 43 aa&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[yjcN]]'', ''[[yjzG]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt     Gene sequence      (+200bp)   ]''' &lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_protein.txt Protein sequence]'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:yjzF_context.gif]]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yjzF_1265530_1265661_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:yjzF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11928]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[proteins of unknown function]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU11928&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU11928&amp;amp;redirect=T BSU11928]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' no entry &lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU11928&amp;amp;redirect=T BSU11928]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' 	&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/C0H3Z0 C0H3Z0]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:'''&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:'''&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' &lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:''' &lt;br /&gt;
** BKE11928 (''[[yjzF]]''::''erm'', available in the BGSC and in [[Jörg Stülke]]'s lab)&lt;br /&gt;
** GP1821 (''[[yjzF]]''::''erm'', available in [[Jörg Stülke]]'s lab)&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
	&lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&lt;br /&gt;
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&amp;amp;cmd=DetailsSearch&amp;amp;term=subtilis+yjzF&amp;amp;log$=activity search PubMed]&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:ScpB_expression.png&amp;diff=207096</id>
		<title>File:ScpB expression.png</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:ScpB_expression.png&amp;diff=207096"/>
				<updated>2015-02-10T11:40:47Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=206016</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=206016"/>
				<updated>2014-07-28T14:42:52Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name''' glaube ich oder &lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:quintos.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:test.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2000 {{PubMed|24696501}} &lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 4000 {{PubMed|24696501}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=206015</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=206015"/>
				<updated>2014-07-28T14:34:28Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name''' glaube ich nicht &lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:quintos.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:test.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2000 {{PubMed|24696501}} &lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 4000 {{PubMed|24696501}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:YgzD1_expression.png&amp;diff=205771</id>
		<title>File:YgzD1 expression.png</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:YgzD1_expression.png&amp;diff=205771"/>
				<updated>2014-06-12T09:43:58Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YgzD&amp;diff=205770</id>
		<title>YgzD</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=YgzD&amp;diff=205770"/>
				<updated>2014-06-12T09:43:28Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{#bank: YgzD}}&lt;br /&gt;
&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:Test.gif&amp;diff=205362</id>
		<title>File:Test.gif</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:Test.gif&amp;diff=205362"/>
				<updated>2014-05-07T07:48:42Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=205361</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=205361"/>
				<updated>2014-05-07T07:47:26Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:quintos.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:test.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2000 {{PubMed|24696501}} &lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 4000 {{PubMed|24696501}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=205360</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=205360"/>
				<updated>2014-05-07T07:46:40Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:quintos.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2000 {{PubMed|24696501}} &lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 4000 {{PubMed|24696501}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=205359</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=205359"/>
				<updated>2014-05-07T07:43:22Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
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&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:quintos.gif]][[Media:Seminare.doc]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2000 {{PubMed|24696501}} &lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 4000 {{PubMed|24696501}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
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				<updated>2014-05-07T07:41:52Z</updated>
		
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				<updated>2014-05-07T07:20:32Z</updated>
		
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				<updated>2014-05-07T07:20:20Z</updated>
		
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				<updated>2014-05-07T07:19:53Z</updated>
		
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	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Main_Page&amp;diff=204357</id>
		<title>Main Page</title>
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				<updated>2014-04-17T11:57:24Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
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&lt;div&gt;&amp;lt;big&amp;gt;Welcome to '''''Subti''Wiki'''! &amp;lt;/big&amp;gt; - ''Bacillus subtilis'' annotation the &amp;quot;wiki&amp;quot; way!&amp;lt;br/&amp;gt;&lt;br /&gt;
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&amp;lt;big&amp;gt;'''Contact:'''&amp;lt;/big&amp;gt; [http://wwwuser.gwdg.de/~genmibio/stuelke.html General Microbiology, University of Göttingen], [mailto:jstuelk@gwdg.de send mail]&lt;br /&gt;
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&amp;lt;big&amp;gt;'''Upcoming events:'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* The next European BACELL meeting will take place in Bratislava from April 29 to April 30, 2014. The meeting will be organized by [[Imrich Barak]], [http://www.imb.savba.sk/dept/mige/bacell/index.php website], [mailto:Imrich.Barak@savba.sk contact], [[program]]&lt;br /&gt;
* [[PTS meeting 2014|PTS 50 - The PTS celebrates 50 years of transport and regulation]], organized by [[Josef Deutscher]], [[Milton Saier]] and [[Jörg Stülke]] will take place from September 07 to 08 in Göttingen, Germany. [[PTS meeting 2014|website]], [http://subtiwiki.uni-goettingen.de/data/Registration_from_PTS50.doc Registration form], [mailto:jstuelk@gwdg.de contact]&lt;br /&gt;
* The [[PTM meeting 2014|1st International Conference on Post-Translational Modifications in Bacteria]], organized by [[Ivan Mijakovic]], Christophe Grangeasse, Alan Kent Nielsen and [[Jörg Stülke]] will take place from September 09 to 10 in Göttingen, Germany. [[PTM meeting 2014|website]], [http://subtiwiki.uni-goettingen.de/data/Registration_form_PTM_2014 Registration form], [mailto:jstuelk@gwdg.de contact]&lt;br /&gt;
* [[Past conferences]]&lt;br /&gt;
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&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''New: Subtiwiki is now on [http://www.facebook.com/pages/SubtiWiki/201847669864806 Facebook] - please give it a +1!'''&amp;lt;/big&amp;gt; &lt;br /&gt;
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&amp;lt;big&amp;gt;'''Now online: A new description of ''Subti''Wiki and its acompanying modules in the [http://nar.oxfordjournals.org/content/40/D1/D1278.full.pdf+html 2014 Database issue of Nucleic Acids Research]'''&amp;lt;/big&amp;gt; &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 24178028 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Paper of the month: April 2014'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* [[DNA repair/ recombination|DNA repair and recombination]] involve the unwinding and digestion of the DNA duplex by the [[AddA]]-[[AddB]] complex from the broken end until they encounter a χ sequence, whereupon the proteins produce a 3′ single-stranded DNA tail onto which they initiate loading of the [[RecA]] protein. Now, two studies from the lab of [[Mark Dillingham]] address the structure of [[AddA]]-[[AddB]] complexed to a χ sequence, and the effect of χ binding for processivity and ATP hydrolysis by [[AddA]]-[[AddB]].&lt;br /&gt;
* '''Relevant ''Subti''Wiki pages:''' [[Mark Dillingham]], [[DNA repair/ recombination]], [[AddA]], [[AddB]]&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 24682829 24670664 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
* [[Previous papers of the month]]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''New procedure for account creation:'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* Due to massive spam attacks, we had to change our policy for the creation of accounts. If you want to create an account, just [mailto:jstuelk@gwdg.de send me a mail]. We will create the account for you as quickly as possible, then you can change your password. This does not affect existing accounts, which remain active. &amp;lt;br/&amp;gt;&lt;br /&gt;
* '''Please remember that all members of the ''Bacillus'' community are invited to create accounts and to contribute to ''Subti''Wiki!''' &lt;br /&gt;
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&amp;lt;big&amp;gt;'''News in ''Subti''Wiki:'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* '''April 2013:''' SubtiWiki uses a new, faster Mediawiki platform! The times for page access are significantly reduced. Enjoy!&lt;br /&gt;
* '''March 2013:''' updated Excel files that map all genes to [[categories]] and [[regulons]] as well as the general updated [http://www.subtiwiki.uni-goettingen.de/wiki/index.php/File:SubtiWiki_id_mapping.xls identity mapping] of all genes were uploaded.&lt;br /&gt;
* '''October 2012''': the [[essential genes]] have been categorized&lt;br /&gt;
* '''September 2012''': 300 mutant strains available at the [http://www.bgsc.org BGSC] were added to the corresponding gene pages&lt;br /&gt;
* '''August 2012''': [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express], the new compagnon of ''Subti''Wiki devoted to gene expression is online! Try it out!&lt;br /&gt;
* '''April 2012:''' Links for expression data under 104 different conditions (based on [http://www.ncbi.nlm.nih.gov/pubmed/22383849 Nicolas ''et al''., 2012]) have been added to the expression section of the individual gene pages&lt;br /&gt;
* '''April 2011:''' [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact], a presentation of [[protein-protein interactions]] in ''B. subtilis'', is online! &lt;br /&gt;
* '''December 2010:''' [http://cellpublisher.gobics.de/scp.html SubtiCellPublisher] allows the conversion of CellDesigner diagrams to navigatable presentations in the style of [[SubtiPathways]]&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
{| border=&amp;quot;0&amp;quot; cellpadding=&amp;quot;1&amp;quot;&lt;br /&gt;
|width=&amp;quot;300&amp;quot;|&amp;lt;big&amp;gt;'''Genes''' (examples)&amp;lt;/big&amp;gt;&lt;br /&gt;
|width=&amp;quot;300&amp;quot;|&amp;lt;big&amp;gt;'''Additional pages'''&amp;lt;/big&amp;gt;&lt;br /&gt;
|- style=&amp;quot;height:20px&amp;quot; &lt;br /&gt;
| ''[[spo0A]]'' || '''[http://subtiwiki.uni-goettingen.de/subtipathways/start.php ''Subti''Pathways]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[glnA]]'' ||  '''[http://subtiwiki.uni-goettingen.de/interact/ SubtInteract]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[rny]]'' ||  '''[http://subtiwiki.uni-goettingen.de/apps/expression/ SubtiExpress]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[psdA]]'' || '''[[Regulons]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[pckA]]'' || '''[[methods]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[dnaA]]'' ||  '''[[metabolism]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[rnpA]]'' ||  '''[[categories]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[sigB]]'' || '''[[genome-wide analyses]]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[ptkA]]'' || '''[[list of plasmids]]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[mreC]]'' || '''[[labs working on Bacillus|labs working on ''Bacillus'']]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[ccpA]]'' || '''[[the people behind SubtiWiki|the people behind ''Subti''Wiki]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[sr1]]'' || '''[[template]]''' &lt;br /&gt;
|-&lt;br /&gt;
|  || &lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Publications describing ''Subti''Wiki and ''Subti''Pathways:'''&amp;lt;/big&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:73%&amp;quot;&amp;gt;&amp;lt;pubmed&amp;gt;20157485 19959575 22096228 24178028 &amp;lt;/pubmed&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Main_Page&amp;diff=204356</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Main_Page&amp;diff=204356"/>
				<updated>2014-04-17T11:56:13Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&amp;lt;big&amp;gt;Welcome to '''''Subti''Wiki'''! &amp;lt;/big&amp;gt; - ''Bacillus subtilis'' annotation the &amp;quot;wiki&amp;quot; way!&amp;lt;br/&amp;gt;&lt;br /&gt;
[[File:Start_page_small.png|right|link=http://www.subtiwiki.uni-goettingen.de/]]&lt;br /&gt;
[[File:Playground_small.png|right|link=http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Playground]]&lt;br /&gt;
[[File:Wish_List_small.png|right|link=http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Wish_List]]&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Contact:'''&amp;lt;/big&amp;gt; [http://wwwuser.gwdg.de/~genmibio/stuelke.html General Microbiology, University of Göttingen], [mailto:jstuelk@gwdg.de send mail]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Upcoming events:'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* The next European BACELL meeting will take place in Bratislava from April 29 to April 30, 2014. The meeting will be organized by [[Imrich Barak]], [http://www.imb.savba.sk/dept/mige/bacell/index.php website], [mailto:Imrich.Barak@savba.sk contact], [[program]]&lt;br /&gt;
* [[PTS meeting 2014|PTS 50 - The PTS celebrates 50 years of transport and regulation]], organized by [[Josef Deutscher]], [[Milton Saier]] and [[Jörg Stülke]] will take place from September 07 to 08 in Göttingen, Germany. [[PTS meeting 2014|website]], [http://subtiwiki.uni-goettingen.de/data/Registration_from_PTS50.doc Registration form], [mailto:jstuelk@gwdg.de contact]&lt;br /&gt;
* The [[PTM meeting 2014|1st International Conference on Post-Translational Modifications in Bacteria]], organized by [[Ivan Mijakovic]], Christophe Grangeasse, Alan Kent Nielsen and [[Jörg Stülke]] will take place from September 09 to 10 in Göttingen, Germany. [[PTM meeting 2014|website]], [http://subtiwiki.uni-goettingen.de/data/Registration_form_PTM_2014 Registration form], [mailto:jstuelk@gwdg.de contact]&lt;br /&gt;
* [[Past conferences]]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''New: Subtiwiki is now on [http://www.facebook.com/pages/SubtiWiki/201847669864806 Facebook] - please give it a +1!'''&amp;lt;/big&amp;gt; &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Now online: A new description of ''Subti''Wiki and its acompanying modules in the [http://nar.oxfordjournals.org/content/40/D1/D1278.full.pdf+html 2014 Database issue of Nucleic Acids Research]'''&amp;lt;/big&amp;gt; &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 24178028 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Paper of the month: April 2014'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* [[DNA repair/ recombination|DNA repair and recombination]] involve the unwinding and digestion of the DNA duplex by the [[AddA]]-[[AddB]] complex from the broken end until they encounter a χ sequence, whereupon the proteins produce a 3′ single-stranded DNA tail onto which they initiate loading of the [[RecA]] protein. Now, two studies from the lab of [[Mark Dillingham]] address the structure of [[AddA]]-[[AddB]] complexed to a χ sequence, and the effect of χ binding for processivity and ATP hydrolysis by [[AddA]]-[[AddB]].&lt;br /&gt;
* '''Relevant ''Subti''Wiki pages:''' [[Mark Dillingham]], [[DNA repair/ recombination]], [[AddA]], [[AddB]]&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 24682829 24670664 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
* [[Previous papers of the month]]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''New procedure for account creation:'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* Due to massive spam attacks, we had to change our policy for the creation of accounts. If you want to create an account, just [mailto:jstuelk@gwdg.de send me a mail]. We will create the account for you as quickly as possible, then you can change your password. This does not affect existing accounts, which remain active. &amp;lt;br/&amp;gt;&lt;br /&gt;
* '''Please remember that all members of the ''Bacillus'' community are invited to create accounts and to contribute to ''Subti''Wiki!''' &lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''News in ''Subti''Wiki:'''&amp;lt;/big&amp;gt; &lt;br /&gt;
* '''April 2013:''' SubtiWiki uses a new, faster Mediawiki platform! The times for page access are significantly reduced. Enjoy!&lt;br /&gt;
* '''March 2013:''' updated Excel files that map all genes to [[categories]] and [[regulons]] as well as the general updated [http://www.subtiwiki.uni-goettingen.de/wiki/index.php/File:SubtiWiki_id_mapping.xls identity mapping] of all genes were uploaded.&lt;br /&gt;
* '''October 2012''': the [[essential genes]] have been categorized&lt;br /&gt;
* '''September 2012''': 300 mutant strains available at the [http://www.bgsc.org BGSC] were added to the corresponding gene pages&lt;br /&gt;
* '''August 2012''': [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express], the new compagnon of ''Subti''Wiki devoted to gene expression is online! Try it out!&lt;br /&gt;
* '''April 2012:''' Links for expression data under 104 different conditions (based on [http://www.ncbi.nlm.nih.gov/pubmed/22383849 Nicolas ''et al''., 2012]) have been added to the expression section of the individual gene pages&lt;br /&gt;
* '''April 2011:''' [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact], a presentation of [[protein-protein interactions]] in ''B. subtilis'', is online! &lt;br /&gt;
* '''December 2010:''' [http://cellpublisher.gobics.de/scp.html SubtiCellPublisher] allows the conversion of CellDesigner diagrams to navigatable presentations in the style of [[SubtiPathways]]&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
{| border=&amp;quot;0&amp;quot; cellpadding=&amp;quot;1&amp;quot;&lt;br /&gt;
|width=&amp;quot;300&amp;quot;|&amp;lt;big&amp;gt;'''Genes''' (examples)&amp;lt;/big&amp;gt;&lt;br /&gt;
|width=&amp;quot;300&amp;quot;|&amp;lt;big&amp;gt;'''Additional pages'''&amp;lt;/big&amp;gt;&lt;br /&gt;
|- style=&amp;quot;height:20px&amp;quot; &lt;br /&gt;
| ''[[spo0A]]'' || '''[http://subtiwiki.uni-goettingen.de/subtipathways/index.php ''Subti''Pathways]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[glnA]]'' ||  '''[http://cellpublisher.gobics.de/subtinteract/startpage/start/ SubtInteract]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[rny]]'' ||  '''[http://subtiwiki.uni-goettingen.de/apps/expression/ SubtiExpress]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[psdA]]'' || '''[[Regulons]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[pckA]]'' || '''[[methods]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[dnaA]]'' ||  '''[[metabolism]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[rnpA]]'' ||  '''[[categories]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[sigB]]'' || '''[[genome-wide analyses]]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[ptkA]]'' || '''[[list of plasmids]]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[mreC]]'' || '''[[labs working on Bacillus|labs working on ''Bacillus'']]'''&lt;br /&gt;
|-&lt;br /&gt;
| ''[[ccpA]]'' || '''[[the people behind SubtiWiki|the people behind ''Subti''Wiki]]''' &lt;br /&gt;
|-&lt;br /&gt;
| ''[[sr1]]'' || '''[[template]]''' &lt;br /&gt;
|-&lt;br /&gt;
|  || &lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Publications describing ''Subti''Wiki and ''Subti''Pathways:'''&amp;lt;/big&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:73%&amp;quot;&amp;gt;&amp;lt;pubmed&amp;gt;20157485 19959575 22096228 24178028 &amp;lt;/pubmed&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=203021</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=203021"/>
				<updated>2014-04-17T08:21:47Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Expression and regulation */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:glmS_context.gif]][[Media:Seminare.doc]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2000 {{PubMed|24696501}} &lt;br /&gt;
** number of protein molecules per cell (complex medium with amino acids, without glucose): 4000 {{PubMed|24696501}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=203004</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=203004"/>
				<updated>2014-04-15T08:16:40Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Expression and regulation */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:glmS_context.gif]][[Media:Seminare.doc]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=202678</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=202678"/>
				<updated>2014-04-08T10:35:28Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:glmS_context.gif]][[Media:Seminare.doc]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=202676</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=202676"/>
				<updated>2014-04-08T10:22:00Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/sandbox.html sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:glmS_context.gif]][[Media:Seminare.doc]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [HELLO BSU00100]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=MciZ&amp;diff=202675</id>
		<title>MciZ</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=MciZ&amp;diff=202675"/>
				<updated>2014-04-08T07:10:54Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Database entries */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[FtsZ]] inhibition peptide &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''mciZ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || '' ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[FtsZ]] inhibition peptide&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || inhibits GTPase activity of [[FtsZ]] and Z ring formation during sporulation&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MciZ MciZ]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 4.8 kDa, y.yy &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 120 bp, 40 aa&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[nudF]]'', ''[[yqkF]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt     Gene sequence      (+200bp)   ]''' &lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_protein.txt Protein sequence]'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:nudF_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell division]]}},&lt;br /&gt;
{{SubtiWiki category|[[short peptides]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:'''&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
This gene was only recently identified. [http://www.ncbi.nlm.nih.gov/sites/entrez/18284588 PubMed]&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' inhibits GTPase activity of [[FtsZ]] and Z ring formation during sporulation {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:''' &lt;br /&gt;
** [[MciZ]]-[[FtsZ]] {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Swiss prot entry:'''&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:'''&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' &lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
	&lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 19680248 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original Publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;,18284588, &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:short peptide]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=MciZ&amp;diff=202674</id>
		<title>MciZ</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=MciZ&amp;diff=202674"/>
				<updated>2014-04-08T07:10:35Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* The gene */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' [[FtsZ]] inhibition peptide &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''mciZ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || '' ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || [[FtsZ]] inhibition peptide&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || inhibits GTPase activity of [[FtsZ]] and Z ring formation during sporulation&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MciZ MciZ]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 4.8 kDa, y.yy &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 120 bp, 40 aa&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[nudF]]'', ''[[yqkF]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt     Gene sequence      (+200bp)   ]''' &lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[http://subtiwiki.uni-goettingen.de/genE_protein.txt Protein sequence]'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:nudF_context.gif]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell division]]}},&lt;br /&gt;
{{SubtiWiki category|[[short peptides]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:'''&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
This gene was only recently identified. [http://www.ncbi.nlm.nih.gov/sites/entrez/18284588 PubMed]&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' inhibits GTPase activity of [[FtsZ]] and Z ring formation during sporulation {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:''' &lt;br /&gt;
** [[MciZ]]-[[FtsZ]] {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=?&amp;amp;redirect=T ?]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=?&amp;amp;redirect=T ?]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Swiss prot entry:'''&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:'''&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' &lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|18284588}}&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
	&lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 19680248 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Original Publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;,18284588, &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:short peptide]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=DnaA&amp;diff=198480</id>
		<title>DnaA</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=DnaA&amp;diff=198480"/>
				<updated>2014-04-02T12:51:55Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' AAA+ ATPase, replication initiation protein &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''dnaA''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''dnaH, dnaJ, dnaK ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || replication initiation protein&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || [[DNA replication]]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00010 dnaA]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DnaA DnaA]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 50 kDa, 6.035  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1338 bp, 446 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[rpmH]]'', ''[[dnaN]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU00010 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU00010 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU00010 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:DnaA_dnaN_yaaA_recF_yaaB_gyrB_context.png]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dnaA_410_1750_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:dnaA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00010]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[DNA replication]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[Spo0A regulon]]}}&lt;br /&gt;
=The [[DnaA regulon]]=&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU00010&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00010&amp;amp;redirect=T BSU00010]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dnaAN.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10065]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' &lt;br /&gt;
** binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase [[DnaC]]&lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' dnaA family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' AAA+ domain&lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
** [[SirA]] displaces [[DnaA]] from the replication origin {{PubMed|19682252}}&lt;br /&gt;
** [[YabA]] inhibits co-operative binding of DnaA to the ''oriC'' DNA {{PubMed|21895792}}&lt;br /&gt;
** DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either [[Soj]], [[YabA]] or [[DnaN]] with the AAA+ domain of DnaA {{PubMed|23909787,22286949}} &lt;br /&gt;
** interaction with [[DnaD]] inhibits the ability of [[DnaA]] to cooperatively bind to DNA {{PubMed|22821970}}&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[DnaA]] assembles into a right-handed helical oligomer built upon interactions between neighbouring AAA+ domains {{PubMed|23909787}} &lt;br /&gt;
** [[Soj]]-[[DnaA]] {{PubMed|22286949}} &lt;br /&gt;
** [[DnaA]]-[[YabA]]  {{PubMed|23909787,12060778}} &lt;br /&gt;
** [[DnaA]]-[[YabA]]-[[DnaN]] {{PubMed|12060778}} &lt;br /&gt;
** [[DnaA]]-[[DnaD]] {{PubMed|22821970,11222620}}&lt;br /&gt;
** [[SirA]]-[[DnaA]] {{PubMed|19682252,21239581}}&lt;br /&gt;
** [[YqaH]]-[[DnaA]] {{PubMed|12060778}} &lt;br /&gt;
** part of the [[primosome]]: [[DnaA]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[DnaB]] {{PubMed|22797751}} &lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:''' &lt;br /&gt;
** throughout the cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00010&amp;amp;redirect=T BSU00010]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''	&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P05648 P05648]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00010]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:'''&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[dnaA]]-[[dnaN]]''  [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dnaA_410_1750_1 dnaA] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** negatively controlled by [[DnaA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2168872 PubMed] and [[Spo0A]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]&lt;br /&gt;
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:'''&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Philippe Noirot]], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]&lt;br /&gt;
* [[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]&lt;br /&gt;
* [[Alan Grossman]], MIT, Cambridge, MA, USA&lt;br /&gt;
* [[Heath Murray]], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 20157337 21035377 21639790 22575476 22797751&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The [[DnaA regulon]]==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 16120674, &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18854156,19011033, 11222620,14651647,17140409 10844689 ,11222620 12060778 16461910 2987848 2168872 11207367, 19737352 19081080 17932079 19968790 19682252 20511500 21097613 21239581 21895792 22286949 22821970 23909787 22396664,21911367&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=DnaA&amp;diff=198468</id>
		<title>DnaA</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=DnaA&amp;diff=198468"/>
				<updated>2014-04-02T12:51:27Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' AAA+ ATPase, replication initiation protein &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''dnaA''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''dnaH, dnaJ, dnaK ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || replication initiation protein&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || [[DNA replication]]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00010 dnaA]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DnaA DnaA]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 50 kDa, 6.035  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1338 bp, 446 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[rpmH]]'', ''[[dnaN]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU00010 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU00010 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU00010 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:DnaA_dnaN_yaaA_recF_yaaB_gyrB_context.png]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dnaA_410_1750_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:dnaA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00010]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[DNA replication]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[Spo0A regulon]]}}&lt;br /&gt;
=The [[DnaA regulon]]=&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU00010&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00010&amp;amp;redirect=T BSU00010]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dnaAN.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10065]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' &lt;br /&gt;
** binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase [[DnaC]]&lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' dnaA family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' AAA+ domain&lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
** [[SirA]] displaces [[DnaA]] from the replication origin {{PubMed|19682252}}&lt;br /&gt;
** [[YabA]] inhibits co-operative binding of DnaA to the ''oriC'' DNA {{PubMed|21895792}}&lt;br /&gt;
** DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either [[Soj]], [[YabA]] or [[DnaN]] with the AAA+ domain of DnaA {{PubMed|23909787,22286949}} &lt;br /&gt;
** interaction with [[DnaD]] inhibits the ability of [[DnaA]] to cooperatively bind to DNA {{PubMed|22821970}}&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
** [[DnaA]] assembles into a right-handed helical oligomer built upon interactions between neighbouring AAA+ domains {{PubMed|23909787}} &lt;br /&gt;
** [[Soj]]-[[DnaA]] {{PubMed|22286949}} &lt;br /&gt;
** [[DnaA]]-[[YabA]]  {{PubMed|23909787,12060778}} &lt;br /&gt;
** [[DnaA]]-[[YabA]]-[[DnaN]] {{PubMed|12060778}} &lt;br /&gt;
** [[DnaA]]-[[DnaD]] {{PubMed|22821970,11222620}}&lt;br /&gt;
** [[SirA]]-[[DnaA]] {{PubMed|19682252,21239581}}&lt;br /&gt;
** [[YqaH]]-[[DnaA]] {{PubMed|12060778}} &lt;br /&gt;
** part of the [[primosome]]: [[DnaA]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[DnaB]] {{PubMed|22797751}} &lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:''' &lt;br /&gt;
** throughout the cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00010&amp;amp;redirect=T BSU00010]&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00010&amp;amp;redirect=T BSU00010]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''	&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P05648 P05648]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00010]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:'''&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[dnaA]]-[[dnaN]]''  [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dnaA_410_1750_1 dnaA] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** negatively controlled by [[DnaA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2168872 PubMed] and [[Spo0A]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]&lt;br /&gt;
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:'''&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
* [[Philippe Noirot]], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]&lt;br /&gt;
* [[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]&lt;br /&gt;
* [[Alan Grossman]], MIT, Cambridge, MA, USA&lt;br /&gt;
* [[Heath Murray]], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 20157337 21035377 21639790 22575476 22797751&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The [[DnaA regulon]]==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 16120674, &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
==Original publications==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18854156,19011033, 11222620,14651647,17140409 10844689 ,11222620 12060778 16461910 2987848 2168872 11207367, 19737352 19081080 17932079 19968790 19682252 20511500 21097613 21239581 21895792 22286949 22821970 23909787 22396664,21911367&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=198283</id>
		<title>Sandbox</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Sandbox&amp;diff=198283"/>
				<updated>2014-04-02T07:23:34Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Database entries */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' glutamine-fructose-6-phosphate transaminase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''glmS''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''gcaA, ybxD ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || glutamine-fructose-6-phosphate transaminase&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || cell wall synthesis &lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &amp;lt;br/&amp;gt;[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sandbox sandbox]'''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 65 kDa, 4.796  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1800 bp, 600 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[glmM]]'', ''[[ybbU]]''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;amp;#91;EMBLCDS:CAB11954&amp;amp;#93;+-newId sequences] &amp;lt;br/&amp;gt; (Barbe ''et al.'', 2009)'''&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:glmS_context.gif]][[Media:Seminare.doc]]&lt;br /&gt;
 &amp;lt;div align=&amp;quot;right&amp;quot;&amp;gt; &amp;lt;small&amp;gt;This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&amp;lt;/small&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:glmS_expression.png|500px]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[cell wall synthesis]]}},&lt;br /&gt;
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},&lt;br /&gt;
{{SubtiWiki category|[[essential genes]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
{{SubtiWiki regulon|[[glmS ribozyme]]}}&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU01780&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T&amp;quot;]&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' no entry&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10948]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate (according to Swiss-Prot) &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:'''&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	&lt;br /&gt;
&lt;br /&gt;
* '''Domains:''' &lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''Cofactor(s):'''&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:'''&lt;br /&gt;
** cytoplasm (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&amp;amp;object=BSU00240&amp;amp;redirect=T BSU00240]&lt;br /&gt;
&lt;br /&gt;
* '''Structure:'''&lt;br /&gt;
**[http://www.pdb.org/pdb/explore/explore.do?structureId=HIV2 HIV2] (from ''Bacillus subtilis'', 100% identity) {{PubMed|13454352}}&lt;br /&gt;
** [http://www.pdb.org/pdb/explore/explore.do?structureId=2VF4 2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) {{PubMed|18295797}}&lt;br /&gt;
** the ribozyme: [http://www.rcsb.org/pdb/explore.do?structureId=3g8s 3G8S], [http://www.rcsb.org/pdb/explore.do?structureId=3G9C 3G9C], [http://www.rcsb.org/pdb/explore.do?structureId=3g8t 3G8T], [http://www.rcsb.org/pdb/explore.do?structureId=3g95 3G95], [http://www.rcsb.org/pdb/explore.do?structureId=3g96 3G96] (all for the ribozyme from ''Bacillus anthracis''), [http://www.rcsb.org/pdb/explore.do?structureId=2HO7 2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39754 P39754]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01780]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.16 2.6.1.16]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''[[ybbP]]-[[ybbR]]-[[glmM]]-[[glmS]]''&lt;br /&gt;
&lt;br /&gt;
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmS_200277_202079_1 glmS] {{PubMed|22383849}}&lt;br /&gt;
&lt;br /&gt;
* '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} &lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine {{PubMed|14343123}} &lt;br /&gt;
** ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([[glmS]] [[ribozyme]])&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' ''glmS'' [[ribozyme]]: glucosamine 6-phosphate binds the leader mRNA, and a [[riboswitch]] with [[ribozyme]] activity cleaves off the ''[[glmS]]'' section from the mRNA, resulting in stopp of transcript elongation&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=PubMed&amp;amp;dopt=Abstract&amp;amp;list_uids=+17981983 PubMed]&lt;br /&gt;
** A [[ncRNA]] is predicted between ''[[glmM]]'' and ''[[glmS]]'' {{PubMed|20525796}}&lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Wade Winkler]], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
==Reviews==&lt;br /&gt;
&amp;lt;pubmed&amp;gt; 18279655 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==The ''glmS'' Ribozyme==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18079181 ,16484375, 16784238 ,15096624 , 16990543 ,17114942 ,16484375 , 15029187, 17283212 , 16298301, 19228039 21317896 21395279 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Other Original Publications==&lt;br /&gt;
'''Additional publications:''' {{PubMed|22211522}} &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 14343123 17981983 ,11160890, 18295797 20525796  &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Abn2&amp;diff=197860</id>
		<title>Abn2</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Abn2&amp;diff=197860"/>
				<updated>2014-02-20T13:41:42Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' endo-1,5-alpha-L-arabinosidase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''abn2''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''yxiA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || endo-1,5-alpha-L-arabinosidase &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || arabinan degradation&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39330 abn2]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 52 kDa, 7.371  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1407 bp, 469 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[yxiB]]'', ''[[yxzL]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU39330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU39330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU39330 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;color:#FF0000&amp;quot; align=&amp;quot;center&amp;quot; | '''Caution: The sequence for this gene in SubtiList contains errors&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:yxiA_context.gif]]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/seb/images/details/abnB_4039466_4040875_-1.png Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:abn2_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39330]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of specific carbon sources]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:''' BSU39330&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yxiA.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11130]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' glycosyl hydrolase 43 family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
** N-terminal catalytic domain with a characteristic β-propeller fold and a C-terminal domain whose function is unknown  {{PubMed|24549757}}&lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' Ca&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; {{PubMed|24549757}}&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:''' &lt;br /&gt;
** extracellular (signal peptide) {{PubMed|18957862,18408032}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.pdb.org/pdb/search/structidSearch.do?structureId=2X8F 2X8F] {{PubMed|20883454}}&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42293 P42293]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39330]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' 3.2.1.99&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''abn2'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]&lt;br /&gt;
** expression is stimulated by arabinose and pectin and repressed by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
** [[CcpA]]: transcription repression,  unknown [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Isabel de Sa-Nogueira]], Lisboa, Portugal [http://www.itqb.unl.pt/research/biology/microbial-genetics homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18607095, 24549757,18408032,18957862 12850135 18408032 20883454&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Abn2&amp;diff=197859</id>
		<title>Abn2</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Abn2&amp;diff=197859"/>
				<updated>2014-02-20T13:40:23Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' endo-1,5-alpha-L-arabinosidase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''abn2''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''yxiA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || endo-1,5-alpha-L-arabinosidase &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || arabinan degradation&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39330 abn2]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 52 kDa, 7.371  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1407 bp, 469 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[yxiB]]'', ''[[yxzL]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU39330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU39330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU39330 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;color:#FF0000&amp;quot; align=&amp;quot;center&amp;quot; | '''Caution: The sequence for this gene in SubtiList contains errors&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:yxiA_context.gif]]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/seb/images/details/abnB_4039466_4040875_-1.png Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:abn2_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39330]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of specific carbon sources]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:'''&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yxiA.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11130]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' glycosyl hydrolase 43 family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
** N-terminal catalytic domain with a characteristic β-propeller fold and a C-terminal domain whose function is unknown  {{PubMed|24549757}}&lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' Ca&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; {{PubMed|24549757}}&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:''' &lt;br /&gt;
** extracellular (signal peptide) {{PubMed|18957862,18408032}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.pdb.org/pdb/search/structidSearch.do?structureId=2X8F 2X8F] {{PubMed|20883454}}&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42293 P42293]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39330]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' 3.2.1.99&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''abn2'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]&lt;br /&gt;
** expression is stimulated by arabinose and pectin and repressed by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
** [[CcpA]]: transcription repression,  unknown [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Isabel de Sa-Nogueira]], Lisboa, Portugal [http://www.itqb.unl.pt/research/biology/microbial-genetics homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18607095, 24549757,18408032,18957862 12850135 18408032 20883454&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Abn2&amp;diff=197857</id>
		<title>Abn2</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=Abn2&amp;diff=197857"/>
				<updated>2014-02-20T13:39:01Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* '''Description:''' endo-1,5-alpha-L-arabinosidase &amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{| align=&amp;quot;right&amp;quot; border=&amp;quot;1&amp;quot; cellpadding=&amp;quot;2&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Gene name'''&lt;br /&gt;
|''abn2''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Synonyms''' || ''yxiA ''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Essential''' || no &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Product''' || endo-1,5-alpha-L-arabinosidase &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Function''' || arabinan degradation&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39330 abn2]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''MW, pI''' || 52 kDa, 7.371  &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''Gene length, protein length''' || 1407 bp, 469 aa &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''Immediate neighbours''' || ''[[yxiB]]'', ''[[yxzL]]''&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&amp;amp;object=BSU39330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&amp;amp;object=BSU39330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&amp;amp;object=BSU39330 DNA_with_flanks]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; style=&amp;quot;background:#FAF8CC;color:#FF0000&amp;quot; align=&amp;quot;center&amp;quot; | '''Caution: The sequence for this gene in SubtiList contains errors&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; | '''Genetic context''' &amp;lt;br/&amp;gt; [[Image:yxiA_context.gif]]&lt;br /&gt;
|-&lt;br /&gt;
|colspan=&amp;quot;2&amp;quot; |'''[http://genome.jouy.inra.fr/seb/images/details/abnB_4039466_4040875_-1.png Expression at a glance]'''&amp;amp;#160;&amp;amp;#160;&amp;amp;#160;{{PubMed|22383849}}&amp;lt;br/&amp;gt;[[Image:abn2_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSUBSU39330]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
__TOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= [[Categories]] containing this gene/protein =&lt;br /&gt;
{{SubtiWiki category|[[utilization of specific carbon sources]]}}&lt;br /&gt;
&lt;br /&gt;
= This gene is a member of the following [[regulons]] =&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=The gene=&lt;br /&gt;
&lt;br /&gt;
=== Basic information ===&lt;br /&gt;
&lt;br /&gt;
* '''Locus tag:'''&lt;br /&gt;
&lt;br /&gt;
===Phenotypes of a mutant ===&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yxiA.html]&lt;br /&gt;
&lt;br /&gt;
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11130]&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=The protein=&lt;br /&gt;
&lt;br /&gt;
=== Basic information/ Evolution ===&lt;br /&gt;
&lt;br /&gt;
* '''Catalyzed reaction/ biological activity:''' &lt;br /&gt;
&lt;br /&gt;
* '''Protein family:''' glycosyl hydrolase 43 family (according to Swiss-Prot)&lt;br /&gt;
&lt;br /&gt;
* '''Paralogous protein(s):'''&lt;br /&gt;
&lt;br /&gt;
=== Extended information on the protein ===&lt;br /&gt;
&lt;br /&gt;
* '''Kinetic information:'''	With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''[[Domains]]:''' &lt;br /&gt;
** N-terminal catalytic domain with a characteristic β-propeller fold and a C-terminal domain whose function is unknown  {{PubMed|24549757}}&lt;br /&gt;
&lt;br /&gt;
* '''Modification:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Cofactors]]:''' Ca&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; {{PubMed|24549757}}&lt;br /&gt;
&lt;br /&gt;
* '''Effectors of protein activity:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[SubtInteract|Interactions]]:'''&lt;br /&gt;
&lt;br /&gt;
* '''[[Localization]]:''' &lt;br /&gt;
** extracellular (signal peptide) {{PubMed|18957862,18408032}}&lt;br /&gt;
&lt;br /&gt;
=== Database entries ===&lt;br /&gt;
&lt;br /&gt;
* '''Structure:''' [http://www.pdb.org/pdb/search/structidSearch.do?structureId=2X8F 2X8F] {{PubMed|20883454}}&lt;br /&gt;
&lt;br /&gt;
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42293 P42293]&lt;br /&gt;
&lt;br /&gt;
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39330]&lt;br /&gt;
&lt;br /&gt;
* '''E.C. number:''' 3.2.1.99&lt;br /&gt;
&lt;br /&gt;
=== Additional information===&lt;br /&gt;
&lt;br /&gt;
=Expression and regulation=&lt;br /&gt;
&lt;br /&gt;
* '''Operon:''' ''abn2'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulation:''' &lt;br /&gt;
** repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]&lt;br /&gt;
** expression is stimulated by arabinose and pectin and repressed by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Regulatory mechanism:''' &lt;br /&gt;
** [[CcpA]]: transcription repression,  unknown [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]&lt;br /&gt;
&lt;br /&gt;
* '''Additional information:''' &lt;br /&gt;
&lt;br /&gt;
=Biological materials =&lt;br /&gt;
&lt;br /&gt;
* '''Mutant:'''&lt;br /&gt;
&lt;br /&gt;
* '''Expression vector:'''&lt;br /&gt;
        &lt;br /&gt;
* '''lacZ fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''GFP fusion:'''&lt;br /&gt;
&lt;br /&gt;
* '''two-hybrid system:''' &lt;br /&gt;
&lt;br /&gt;
* '''Antibody:'''&lt;br /&gt;
&lt;br /&gt;
=Labs working on this gene/protein=&lt;br /&gt;
&lt;br /&gt;
[[Isabel de Sa-Nogueira]], Lisboa, Portugal [http://www.itqb.unl.pt/research/biology/microbial-genetics homepage]&lt;br /&gt;
&lt;br /&gt;
=Your additional remarks=&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=References=&lt;br /&gt;
&amp;lt;pubmed&amp;gt;18607095, 24549757,18408032,18957862 12850135 18408032 20883454&amp;lt;/pubmed&amp;gt;&lt;br /&gt;
[[Category:Protein-coding genes]]&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:Mutant.jpg&amp;diff=197649</id>
		<title>File:Mutant.jpg</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=File:Mutant.jpg&amp;diff=197649"/>
				<updated>2014-02-12T14:18:56Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=User:Raphael2215&amp;diff=197648</id>
		<title>User:Raphael2215</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=User:Raphael2215&amp;diff=197648"/>
				<updated>2014-02-12T14:16:55Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: Created page with &amp;quot;{| align=&amp;quot;left&amp;quot; border=&amp;quot;5&amp;quot; cellpadding=&amp;quot;5&amp;quot;  |- |style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''user name''' |Raphael Michna ([mailto:rmichna@gwdg.de send mail]) |- |style=&amp;quot;back...&amp;quot;&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{| align=&amp;quot;left&amp;quot; border=&amp;quot;5&amp;quot; cellpadding=&amp;quot;5&amp;quot; &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''user name'''&lt;br /&gt;
|Raphael Michna ([mailto:rmichna@gwdg.de send mail])&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''status''' || PhD student &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''nickname''' || Socke &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''hair color''' || brown&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''weight''' || 80 kg&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''shoe size''' || 43 &lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;| '''hobbies''' ||  unknown&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#ABCDEF;&amp;quot; align=&amp;quot;center&amp;quot;|'''dedication''' || ''Subti''Wiki &amp;amp; [[SubtiPathways|''Subti''Pathways]]&lt;br /&gt;
|-&lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''collaborating with ''' &lt;br /&gt;
|style=&amp;quot;background:#FAF8CC;&amp;quot; align=&amp;quot;center&amp;quot;|'''[[User:Aschmei|Aschmei]] and [[Sroppel]] '''&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[Image:mutant.jpg|400px|thumb|left|]]&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=SubtiPathways&amp;diff=197556</id>
		<title>SubtiPathways</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=SubtiPathways&amp;diff=197556"/>
				<updated>2014-01-30T14:12:34Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* metal ion homeostasis */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:Pathway_start_page_small.png|right|link=http://subtiwiki.uni-goettingen.de/subtipathways/start.php]]&lt;br /&gt;
[[File:Legende.jpg|250px|thumb|right|'''legend for ''Subti''Pathways &amp;lt;br/&amp;gt;graphical notation: click to enlarge''']]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;big&amp;gt;'''''Subti''Pathways''' is a model of '''''B. subtilis''''' metabolism and regulation in '''SBML/SBGN''' (Systems Biology Markup Language/ Graphical Notation). There is a color code for key metabolites (see the legend on the right side).&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;It can be used like Google Maps (scroll and zoom to the pathways). The markers of proteins and compounds listed on the right side in each pathway are containing links to the corresponding ''Subti''Wiki and PubChem pages, respectively. There is a short description for each protein in the bubbles, too.&amp;lt;/big&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;big&amp;gt;'''Contact:'''&amp;lt;/big&amp;gt; [http://wwwuser.gwdg.de/~genmibio/stuelke.html General Microbiology, University of Göttingen], [mailto:jstuelk@gwdg.de send mail]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Now online: A description of ''Subti''Wiki, ''Subti''Pathways, and ''Subt''Interact in the 2012 Database issue of Nucleic Acids Research'''&amp;lt;/big&amp;gt; &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 22096228 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
__NOTOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== A collection of important papers on ''B. subtilis'' [[metabolism]] can be found [[metabolism|here]] ==&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
== Carbon metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=cent Central carbon metabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=carb Utilization of different carbon sources] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Nitrogen and amino acid metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=glut Ammonium assimilation and glutamate metabolism (incl. glutamine and arginine)] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=alan Alanine, glycine, serine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=aspa Aspartate, asparagine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=cyst Cysteine, methionine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=hist Histidine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=isol Isoleucine, valine, leucine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=lysi Lysine, threonine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=tryp Phenylalanine, tyrosine, tryptophan] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=prol Proline] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Utilization of other nitrogen sources] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Lipid metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=fatt Fatty acid and phospholipid biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_degradation.html Fatty acid degradation] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Nucleotides ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Gene regulation of nucleotide metabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=nucl Nucleoside catabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=meta Purine biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=puri Purine salvage pathways] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=pyri Pyrimidine metabolism] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Metabolism of cofactors ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=synt CoA synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=fola Folate biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=mena Menaquinone] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=ribo Riboflavin and FAD synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin synthesis] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Other pathways ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/murein/index.html Murein recycling] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=stre Stress responses] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Sulfate assimilation] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=trna tRNA charging] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm formation] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/secretion/index.html Protein secretion] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=lipo Lipoate biosynthesis ] ===&lt;br /&gt;
&lt;br /&gt;
== Publications describing ''Subti''Wiki and ''Subti''Pathways ==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19959575 22096228&amp;lt;/pubmed&amp;gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=SubtiPathways&amp;diff=197555</id>
		<title>SubtiPathways</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=SubtiPathways&amp;diff=197555"/>
				<updated>2014-01-30T13:59:18Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* Phenylalanine, tyrosine, tryptophan */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:Pathway_start_page_small.png|right|link=http://subtiwiki.uni-goettingen.de/subtipathways/start.php]]&lt;br /&gt;
[[File:Legende.jpg|250px|thumb|right|'''legend for ''Subti''Pathways &amp;lt;br/&amp;gt;graphical notation: click to enlarge''']]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;big&amp;gt;'''''Subti''Pathways''' is a model of '''''B. subtilis''''' metabolism and regulation in '''SBML/SBGN''' (Systems Biology Markup Language/ Graphical Notation). There is a color code for key metabolites (see the legend on the right side).&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;It can be used like Google Maps (scroll and zoom to the pathways). The markers of proteins and compounds listed on the right side in each pathway are containing links to the corresponding ''Subti''Wiki and PubChem pages, respectively. There is a short description for each protein in the bubbles, too.&amp;lt;/big&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;big&amp;gt;'''Contact:'''&amp;lt;/big&amp;gt; [http://wwwuser.gwdg.de/~genmibio/stuelke.html General Microbiology, University of Göttingen], [mailto:jstuelk@gwdg.de send mail]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Now online: A description of ''Subti''Wiki, ''Subti''Pathways, and ''Subt''Interact in the 2012 Database issue of Nucleic Acids Research'''&amp;lt;/big&amp;gt; &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 22096228 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
__NOTOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== A collection of important papers on ''B. subtilis'' [[metabolism]] can be found [[metabolism|here]] ==&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
== Carbon metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=cent Central carbon metabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=carb Utilization of different carbon sources] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Nitrogen and amino acid metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=glut Ammonium assimilation and glutamate metabolism (incl. glutamine and arginine)] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=alan Alanine, glycine, serine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=aspa Aspartate, asparagine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=cyst Cysteine, methionine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=hist Histidine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=isol Isoleucine, valine, leucine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=lysi Lysine, threonine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=tryp Phenylalanine, tyrosine, tryptophan] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=prol Proline] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Utilization of other nitrogen sources] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Lipid metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=fatt Fatty acid and phospholipid biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_degradation.html Fatty acid degradation] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Nucleotides ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Gene regulation of nucleotide metabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=nucl Nucleoside catabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=meta Purine biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=puri Purine salvage pathways] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=pyri Pyrimidine metabolism] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Metabolism of cofactors ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=synt CoA synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=fola Folate biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=mena Menaquinone] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=ribo Riboflavin and FAD synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin synthesis] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Other pathways ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/murein/index.html Murein recycling] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=stre Stress responses] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Sulfate assimilation] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=trna tRNA charging] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm formation] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/secretion/index.html Protein secretion] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://cellpublisher.gobics.de/view/GqbblRJkBH metal ion homeostasis] ===&lt;br /&gt;
&lt;br /&gt;
== Publications describing ''Subti''Wiki and ''Subti''Pathways ==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19959575 22096228&amp;lt;/pubmed&amp;gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	<entry>
		<id>http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=SubtiPathways&amp;diff=197554</id>
		<title>SubtiPathways</title>
		<link rel="alternate" type="text/html" href="http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=SubtiPathways&amp;diff=197554"/>
				<updated>2014-01-30T13:58:07Z</updated>
		
		<summary type="html">&lt;p&gt;Raphael2215: /* tRNA charging */&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:Pathway_start_page_small.png|right|link=http://subtiwiki.uni-goettingen.de/subtipathways/start.php]]&lt;br /&gt;
[[File:Legende.jpg|250px|thumb|right|'''legend for ''Subti''Pathways &amp;lt;br/&amp;gt;graphical notation: click to enlarge''']]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;big&amp;gt;'''''Subti''Pathways''' is a model of '''''B. subtilis''''' metabolism and regulation in '''SBML/SBGN''' (Systems Biology Markup Language/ Graphical Notation). There is a color code for key metabolites (see the legend on the right side).&amp;lt;br/&amp;gt;&amp;lt;br/&amp;gt;It can be used like Google Maps (scroll and zoom to the pathways). The markers of proteins and compounds listed on the right side in each pathway are containing links to the corresponding ''Subti''Wiki and PubChem pages, respectively. There is a short description for each protein in the bubbles, too.&amp;lt;/big&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;big&amp;gt;'''Contact:'''&amp;lt;/big&amp;gt; [http://wwwuser.gwdg.de/~genmibio/stuelke.html General Microbiology, University of Göttingen], [mailto:jstuelk@gwdg.de send mail]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;big&amp;gt;'''Now online: A description of ''Subti''Wiki, ''Subti''Pathways, and ''Subt''Interact in the 2012 Database issue of Nucleic Acids Research'''&amp;lt;/big&amp;gt; &lt;br /&gt;
&amp;lt;pubmed&amp;gt; 22096228 &amp;lt;/pubmed&amp;gt;&lt;br /&gt;
__NOTOC__&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== A collection of important papers on ''B. subtilis'' [[metabolism]] can be found [[metabolism|here]] ==&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
== Carbon metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=cent Central carbon metabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=carb Utilization of different carbon sources] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Nitrogen and amino acid metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=glut Ammonium assimilation and glutamate metabolism (incl. glutamine and arginine)] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=alan Alanine, glycine, serine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=aspa Aspartate, asparagine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=cyst Cysteine, methionine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=hist Histidine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=isol Isoleucine, valine, leucine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=lysi Lysine, threonine] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/phenyl_tyr_tryp.html Phenylalanine, tyrosine, tryptophan] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=prol Proline] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Utilization of other nitrogen sources] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Lipid metabolism ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=fatt Fatty acid and phospholipid biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_degradation.html Fatty acid degradation] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Nucleotides ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Gene regulation of nucleotide metabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=nucl Nucleoside catabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=meta Purine biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=puri Purine salvage pathways] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=pyri Pyrimidine metabolism] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Metabolism of cofactors ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=synt CoA synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=fola Folate biosynthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=mena Menaquinone] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=ribo Riboflavin and FAD synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin synthesis] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin synthesis] ===&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br/&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Other pathways ==&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/murein/index.html Murein recycling] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=stre Stress responses] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Sulfate assimilation] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/subtipathways/index.php?pathway=trna tRNA charging] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm formation] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://subtiwiki.uni-goettingen.de/pathways/secretion/index.html Protein secretion] ===&lt;br /&gt;
&lt;br /&gt;
=== [http://cellpublisher.gobics.de/view/GqbblRJkBH metal ion homeostasis] ===&lt;br /&gt;
&lt;br /&gt;
== Publications describing ''Subti''Wiki and ''Subti''Pathways ==&lt;br /&gt;
&amp;lt;pubmed&amp;gt;19959575 22096228&amp;lt;/pubmed&amp;gt;&lt;/div&gt;</summary>
		<author><name>Raphael2215</name></author>	</entry>

	</feed>