SubtiWiki - User contributions [en]

SubtiWiki:Community Portal

2009-11-20T11:49:23Z

Leendert Hamoen: /* Marie Curie short term training fellowships: */

= Fellowships =

Please enter any fellowships in the space provided below:

= Positions =

Please enter any open positions in the space provided below:

October 5, 2009

Postdoc position in Molecular Genetics, University of Groningen, the Netherlands
at the Groningen Biomolecular Sciences and Biotechnology Institute, the Netherlands

The Postdoc will take part in a large collaborative project (BetNet) of the GBB with the Institute of Environmental Sciences (CML, Leiden University), the Mathematical Institute (MI, Leiden University), and the Leiden institute of Advanced Computer Science (LIACS, Leiden University), funded by the Dutch Science Foundation (NWO), within the Computational Lif Sciences programme. The goal of this project is to resolve the dynamics of gene regulation networks, by using the quantitative demands on the shape of bacterial bet-hedging strategy distributions that are imposed by fluctuating selection environments. As a model system, we study the adaptation of the distribution of sporulation-initiation timing in B.subtilis to randomly changing environments and cue reliability.

The project at Molecular Genetics mainly focuses on the experimental part, involving the design and execution of specific selection experiments in B. subtilis, the characterization of evolved strains by transcriptome analysis, time lapse microscopy, FACS, and whole genome resequencing of selected strains. For the modeling, evolution biology and bioinformnatics we will closely collaborate with the partners in Leiden.

Requirements: PhD degree in Molecular Microbiology or related disciplines. Preferably experience in working with Bacillus subtilis.

Appointment
We offer a temporary full time position for a period of 2.5 years. Monthly gross salary is from €2379,- to €3755,- a month depending on qualification and experience of the applicant. Appointment will be under the term of the Collective Labour Agreement of Dutch Universities (CAO Nederlandse Universiteiten).

Further information and a full project desciption can be obtained by mailing o.p.kuipers@rug.nl.
Applications: send a CV + accompanying letter to o.p.kuipers@rug.nl before October 31.
The positions are at the Molecular Genetics Dept., University of Groningen, Kerklaan 30, 9728 XG Haren, the Netherlands.

-----------------------------------------------------------------------

April 3, 2009

Ph.D. positions in Jan Kok's lab (Groningen):

Dear colleagues,

I would really appreciate if you would disseminate the following to _excellen_t students that you may be guiding at the moment or that have just finished and are looking for a PhD position: I have a number of possible bursary proposals on /Lactococcus lactis/ as well as /Enterococcus faecalis/ research in the frame of our yearly Ubbo Emmius Scholarship awards. See below for more information.

With best regards,
Jan Kok
-----------------------------------------------------------------------

Main Page

2009-11-20T11:48:17Z

Leendert Hamoen:

<big>Welcome to '''''Subti''Wiki'''! </big> - ''Bacillus subtilis'' annotation the "wiki" way! 

[[File:Start_page_small.png|right|link=http://www.subtiwiki.uni-goettingen.de/]]
[[File:Playground_small.png|right|link=http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Playground]]
[[File:Wish_List_small.png|right|link=http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Wish_List]]
Pages for all genes are available, but the information is not complete. Besides gene pages, there are also pages on other topics available. 

<big>'''Contact'''</big>
*[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage of General Microbiology, University of Göttingen]
* [mailto:jstuelk@gwdg.de send mail]

{| border="0" cellpadding="1"
|width="300"|<big>'''Genes''' (examples)</big>
|width="300"|<big>'''Additional pages'''</big>
|- style="height:20px"
| ||
|-
| ''[[eno]]'' || '''[http://subtipathways.uni-goettingen.de ''Subti''Pathways]'''
|-
| ''[[glnA]]'' || '''[[Lists of genes, proteins and RNAs]]'''
|-
| ''[[pckA]]'' || '''[[methods]]'''
|-
| ''[[ptkA]]'' || '''[[list of plasmids]]'''
|-
| ''[[mreC]]'' || '''[[labs working on Bacillus|labs working on ''Bacillus'']]'''
|-
| ''[[ccpA]]'' || '''[[the people behind SubtiWiki|the people behind ''Subti''Wiki]]'''
|-
| ''[[sr1]]'' || '''[[templates]]'''
|-
| ''[[gapA]]'' ||
|-
| ''[[dnaA]]'' ||
|-
| ''[[ccpN]]'' ||
|}

SepF

2009-05-02T14:40:07Z

Leendert Hamoen: /* Your additional remarks */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
SepF binds to FtsZ

* '''Localization:''' cytoplasm (according to Swiss-Prot)
Septum

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Leendert Hamoen]], CBCB, Newcastle University, UK

=Your additional remarks=
''SepF'' mutation is synthetic lethal in combination with an ''ezrA'' mutation or an ''ftsA'' mutation.

=References=

# Hamoen et al. (2006) SepF, a novel FtsZ-interacting protein required for a late step in cell division ''Mol. Microbiol.'' '''59(3):''' 989-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 Pubmed]
# Ishikawa et al. (2006) A new FtsZ-interacting protein, YlmF, complements the activity of FtsA during progression of cell division in ''Bacillus subtilis'' ''Mol. Microbiol.'' '''60(6):''' 1364-80. [http://www.ncbi.nlm.nih.gov/sites/entrez/16796675 Pubmed]

SepF

2009-05-02T14:39:38Z

Leendert Hamoen: /* Your additional remarks */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
SepF binds to FtsZ

* '''Localization:''' cytoplasm (according to Swiss-Prot)
Septum

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Leendert Hamoen]], CBCB, Newcastle University, UK

=Your additional remarks=
''SepF'' mutation is synthetic lethal when combined with an ''ezrA'' mutation or an ''ftsA'' mutation.

=References=

# Hamoen et al. (2006) SepF, a novel FtsZ-interacting protein required for a late step in cell division ''Mol. Microbiol.'' '''59(3):''' 989-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 Pubmed]
# Ishikawa et al. (2006) A new FtsZ-interacting protein, YlmF, complements the activity of FtsA during progression of cell division in ''Bacillus subtilis'' ''Mol. Microbiol.'' '''60(6):''' 1364-80. [http://www.ncbi.nlm.nih.gov/sites/entrez/16796675 Pubmed]

SepF

2009-05-02T14:34:56Z

Leendert Hamoen: /* References */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
SepF binds to FtsZ

* '''Localization:''' cytoplasm (according to Swiss-Prot)
Septum

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Leendert Hamoen]], CBCB, Newcastle University, UK

=Your additional remarks=

=References=

# Hamoen et al. (2006) SepF, a novel FtsZ-interacting protein required for a late step in cell division ''Mol. Microbiol.'' '''59(3):''' 989-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 Pubmed]
# Ishikawa et al. (2006) A new FtsZ-interacting protein, YlmF, complements the activity of FtsA during progression of cell division in ''Bacillus subtilis'' ''Mol. Microbiol.'' '''60(6):''' 1364-80. [http://www.ncbi.nlm.nih.gov/sites/entrez/16796675 Pubmed]

SepF

2009-05-02T14:31:22Z

Leendert Hamoen: /* References */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
SepF binds to FtsZ

* '''Localization:''' cytoplasm (according to Swiss-Prot)
Septum

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Leendert Hamoen]], CBCB, Newcastle University, UK

=Your additional remarks=

=References=

# Hamoen et al. (2006) SepF, a novel FtsZ-interacting protein required for a late step in cell division ''Mol. Microbiol.'' '''59(3):''' 989-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/16420366 Pubmed]

SepF

2009-05-02T14:25:17Z

Leendert Hamoen: /* Labs working on this gene/protein */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
SepF binds to FtsZ

* '''Localization:''' cytoplasm (according to Swiss-Prot)
Septum

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Leendert Hamoen]], CBCB, Newcastle University, UK

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

SepF

2009-05-02T14:18:32Z

Leendert Hamoen: /* Labs working on this gene/protein */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
SepF binds to FtsZ

* '''Localization:''' cytoplasm (according to Swiss-Prot)
Septum

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
Leendert Hamoen, CBCB, Newcastle University, UK

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

SepF

2009-05-02T14:16:37Z

Leendert Hamoen: /* Extended information on the protein */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
SepF binds to FtsZ

* '''Localization:''' cytoplasm (according to Swiss-Prot)
Septum

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

SepF

2009-05-02T14:14:59Z

Leendert Hamoen: /* Basic information/ Evolution */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
Cell division

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

SepF

2009-05-02T14:14:19Z

Leendert Hamoen: /* Phenotypes of a mutant */

* '''Description:''' part of the divisome 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sepF ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylmF''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || FtsZ-interacting protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || proper execution of septum synthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.863
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylmE]]'', ''[[ylmG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13413]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ylmF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===
Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylmDEFGH.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13375]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15390]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Leendert Hamoen

2009-05-02T14:04:20Z

Leendert Hamoen:

= Lab head: Leendert Hamoen=
'''Additional information'''
* [mailto:l.hamoen@newcastle.ac.uk send mail]
* location: Newcastle, UK
* Homepage: [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Hamoen%2BLW%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =
cell division, protein localization, gene regulation

= Open positions =