SubtiWiki - User contributions [en]

ProH

2012-02-28T09:29:00Z

Hoffmant: /* References */

* '''Description:''' pyrroline-5-carboxylate reductase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''proH''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yoxE ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || pyrroline-5-carboxylate reductase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || osmoadaptive de novo production of proline
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/proline.html Pro], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 7.754
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 891 bp, 297 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[proJ]]'', ''[[rtp]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13741]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:proH_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
{{SubtiWiki category|[[coping with hyper-osmotic stress]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU18480

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11049]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)

* '''Protein family:''' glutamate 5-kinase family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[ProI]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P14383 P14383]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18480]

* '''E.C. number:''' [http://www.expasy.org/enzyme/1.5.1.2 1.5.1.2]

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[proH]]-[[proJ]]'' {{PubMed|21296969}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21784929}}

* '''Regulation:'''
** expressed under conditions of osmotic stress {{PubMed|21784929}}
** induced by cold stress {{PubMed|21296969}}

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
'''Additional publications:''' {{PubMed|21784929}}
<pubmed> ,11418582, 21296969</pubmed>

[[Category:Protein-coding genes]]

ProH

2012-02-28T09:28:23Z

Hoffmant: /* References */