SubtiWiki - User contributions [en]

Anne Galinier

2008-12-15T21:26:15Z

88.70.2.125: /* Publications */

= Lab head: Anne Galinier=
'''Additional information'''
* [mailto:galinier@ibsm.cnrs-mrs.fr send mail]
* location: Marseille, France
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=%22Galinier+A%22+AND+bacillus&log$=activity Pubmed]

= Main research interests =

carbon catabolite repression, phosphotransferase system (PTS), protein phosphorylation

= Open positions =

Anne Galinier

2008-12-15T21:25:19Z

88.70.2.125: New page: = Lab head: Anne Galinier= '''Additional information''' * [mailto:galinier@ibsm.cnrs-mrs.fr send mail] * location: Marseille, France * Homepage: not available = Publications = [http://ww...

= Lab head: Anne Galinier=
'''Additional information'''
* [mailto:galinier@ibsm.cnrs-mrs.fr send mail]
* location: Marseille, France
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Gorke%2BB%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

carbon catabolite repression, phosphotransferase system (PTS), protein phosphorylation

= Open positions =

Yasutaro Fujita

2008-12-15T21:15:10Z

88.70.2.125: /* Main research interests */

= Lab head: Yasutaro Fujita=
'''Additional information'''
* [mailto:yfujita@bt.fubt.fukuyama-u.ac.jp send mail]
* location: Fukuyama, Japan
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=%22Fujita+Y%22+AND+bacillus&log$=activity Pubmed]

= Main research interests =

sugar metabolism, fatty acid metabolism, carbon catabolite repression

= Open positions =

Yasutaro Fujita

2008-12-15T21:14:18Z

88.70.2.125: /* Publications */

Yasutaro Fujita

2008-12-15T21:13:13Z

88.70.2.125: /* Publications */

= Lab head: Yasutaro Fujita=
'''Additional information'''
* [mailto:yfujita@bt.fubt.fukuyama-u.ac.jp send mail]
* location: Fukuyama, Japan
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=%22Fujita+Y%22[Author]+AND+bacillus&log$=activity Pubmed]

= Main research interests =

sugar metabolism, carbon catabolite repression

= Open positions =

Yasutaro Fujita

2008-12-15T21:11:34Z

88.70.2.125:

= Lab head: Yasutaro Fujita=
'''Additional information'''
* [mailto:yfujita@bt.fubt.fukuyama-u.ac.jp send mail]
* location: Fukuyama, Japan
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/portal/utils/pageresolver.fcgi?log$=activity&recordid=1229375443161558 Pubmed]

= Main research interests =

sugar metabolism, carbon catabolite repression

= Open positions =

Milton H. Saier

2008-12-15T21:03:35Z

88.70.2.125: /* Publications */

= Lab head: Milton H. Saier, Jr.=
'''Additional information'''
* [mailto:saier@biomail.ucsd.edu send mail]
* location: San Diego, California, USA
* Homepage: [http://biology.ucsd.edu/faculty/saier.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Saier%2BMH%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

sugar transport, phosphotransferase system (PTS), carbon catabolite repression

= Open positions =

Milton H. Saier

2008-12-15T21:03:06Z

88.70.2.125:

= Lab head: Milton H. Saier, Jr.=
'''Additional information'''
* [mailto:saier@biomail.ucsd.edu send mail]
* location: San Diego, California, USA
* Homepage: [http://biology.ucsd.edu/faculty/saier.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Saier%2BMJ%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

sugar transport, phosphotransferase system (PTS), carbon catabolite repression

= Open positions =

Ciaran Condon

2008-12-15T20:59:57Z

88.70.2.125: /* Main research interests */

= Lab head: Ciaran Condon=
'''Additional information'''
* [mailto:ciaran.condon@ibpc.fr send mail]
* location: Paris, France
* Homepage: [http://www.ibpc.fr/UPR9073/condon/index_en.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Condon%2BC%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

mRNA processing, RNA degradation

= Open positions =

Ciaran Condon

2008-12-15T20:59:35Z

88.70.2.125:

= Lab head: Ciaran Condon=
'''Additional information'''
* [mailto:ciaran.condon@ibpc.fr send mail]
* location: Paris, France
* Homepage: [http://www.ibpc.fr/UPR9073/condon/index_en.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Condon%2BC%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

sugar transport, phosphotransferase system (PTS), protein phosphorylation

= Open positions =

David Bechhofer

2008-12-15T20:57:20Z

88.70.2.125: /* Lab head: DAvid Bechhofer */

= Lab head: David Bechhofer=
'''Additional information'''
* [mailto:david.bechhofer@mssm.edu send mail]
* location: New York, USA
* Homepage: [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Bechhofer%2BD%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

RNA degradation

= Open positions =

David Bechhofer

2008-12-15T20:57:07Z

88.70.2.125: /* Lab head: DAvid Bechhofer */

= Lab head: DAvid Bechhofer=
'''Additional information'''
* [mailto:david.bechhofer@mssm.edu send mail]
* location: New York, USA
* Homepage: [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Bechhofer%2BD%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

RNA degradation

= Open positions =

David Bechhofer

2008-12-15T20:56:35Z

88.70.2.125:

= Lab head: DAvid Bechhofer=
'''Additional information'''
* [mailto: david.bechhofer@mssm.edu send mail]
* location: New York, USA
* Homepage: [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Bechhofer%2BD%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

RNA degradation

= Open positions =

Harald Putzer

2008-12-15T20:54:25Z

88.70.2.125:

= Lab head: Harald Putzer=
'''Additional information'''
* [mailto:harald.putzer@ibpc.fr send mail]
* location: Paris, France
* Homepage: [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Putzer%2BH%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

mRNA processing, RNA degradation

= Open positions =

Harald Putzer

2008-12-15T20:53:54Z

88.70.2.125:

= Lab head: Harald Putzer=
'''Additional information'''
* [mailto:harald.putzer@ibpc.fr send mail]
* location: Paris, France
* Homepage: [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Putzer%2BH%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

mRNA processing, RNA degradation

= Open positions =

Richard Brennan

2008-12-15T20:50:27Z

88.70.2.125: /* Main research interests */

= Lab head: Richard Brennan=
'''Additional information'''
* [mailto:rgbrenna@mdanderson.org send mail]
* location: Houston, Texas, USA
* Homepage: [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Brennan%2BRG%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

structural biology, carbon catabolite repression, small RNAs

= Open positions =

Richard Brennan

2008-12-15T20:49:04Z

88.70.2.125:

= Lab head: Richard Brennan=
'''Additional information'''
* [mailto:rgbrenna@mdanderson.org send mail]
* location: Houston, Texas, USA
* Homepage: [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Brennan%2BRG%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

sugar transport, phosphotransferase system (PTS), protein phosphorylation

= Open positions =

Wolfgang Hillen

2008-12-15T20:45:56Z

88.70.2.125:

= Lab head: Wolfgang Hillen=
'''Additional information'''
* [mailto:whillen@biologie.uni-erlangen.de send mail]
* location: Erlangen, Germany
* Homepage: [http://www.biologie.uni-erlangen.de/mibi/index2.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Hillen%2BW%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

carbon catabolite repression, protein-protein interactions

= Open positions =

Michael Hecker

2008-12-15T20:42:51Z

88.70.2.125:

= Lab head: Michael Hecker=
'''Additional information'''
* [mailto:Hecker@uni-greifswald.de send mail]
* location: Greifswald, Germany
* Homepage: [http://www.mikrobiologie.uni-greifswald.de/index.php?id=20 link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Hecker%2BM%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

stress responses, proteomics, protein degradation, systems biology, protein phosphorylation

= Open positions =

Uwe Völker

2008-12-15T20:39:13Z

88.70.2.125:

= Lab head: Uwe Völker=
'''Additional information'''
* [mailto:voelker@uni-greifswald.de send mail]
* location: Greifswald, Germany
* Homepage: [http://www.medizin.uni-greifswald.de/funkgenom/about/voelker/cv.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Volker%2BU%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

stress responses, systems biology

= Open positions =

Boris Görke

2008-12-15T20:36:09Z

88.70.2.125: /* Lab head: Boris Görke */

= Lab head: Boris Görke=
'''Additional information'''
* [mailto:bgoerke@gwdg.de send mail]
* location: Göttingen, Germany
* Homepage: [http://wwwuser.gwdg.de/~genmibio/goerke.html link]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Gorke%2BB%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

carbon catabolite repression, phosphotransferase system (PTS), protein phosphorylation, small RNAs

= Open positions =

Boris Görke

2008-12-15T20:34:48Z

88.70.2.125: /* Publications */

= Lab head: Boris Görke=
'''Additional information'''
* [mailto:bgoerke@gwdg.de send mail]
* location: Göttingen, Germany
* Homepage: [http://wwwuser.gwdg.de/~genmibio/goerke.html homepage]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Gorke%2BB%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

carbon catabolite repression, phosphotransferase system (PTS), protein phosphorylation, small RNAs

= Open positions =

Boris Görke

2008-12-15T20:33:52Z

88.70.2.125: /* Publications */

= Lab head: Boris Görke=
'''Additional information'''
* [mailto:bgoerke@gwdg.de send mail]
* location: Göttingen, Germany
* Homepage: [http://wwwuser.gwdg.de/~genmibio/goerke.html homepage]

= Publications =

= Main research interests =

carbon catabolite repression, phosphotransferase system (PTS), protein phosphorylation, small RNAs

= Open positions =

Boris Görke

2008-12-15T20:33:22Z

88.70.2.125: /* Publications */

= Lab head: Boris Görke=
'''Additional information'''
* [mailto:bgoerke@gwdg.de send mail]
* location: Göttingen, Germany
* Homepage: [http://wwwuser.gwdg.de/~genmibio/goerke.html homepage]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=Gorke%2BB%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

carbon catabolite repression, phosphotransferase system (PTS), protein phosphorylation, small RNAs

= Open positions =

Boris Görke

2008-12-15T20:32:41Z

88.70.2.125:

= Lab head: Boris Görke=
'''Additional information'''
* [mailto:bgoerke@gwdg.de send mail]
* location: Göttingen, Germany
* Homepage: [http://wwwuser.gwdg.de/~genmibio/goerke.html homepage]

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%Gorke%2BB%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

carbon catabolite repression, phosphotransferase system (PTS), protein phosphorylation, small RNAs

= Open positions =

Michel Debarbouille

2008-12-15T20:30:01Z

88.70.2.125: New page: = Lab head: Josef Deutscher= '''Additional information''' * [mailto:Josef.Deutscher@grignon.inra.fr send mail] * location: Paris-Grignon, France * Homepage: not available = Publications ...

= Lab head: Josef Deutscher=
'''Additional information'''
* [mailto:Josef.Deutscher@grignon.inra.fr send mail]
* location: Paris-Grignon, France
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Deutscher%2BJ%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

sugar transport, phosphotransferase system (PTS), protein phosphorylation

= Open positions =

Yasutaro Fujita

2008-12-15T20:29:29Z

Milton H. Saier

2008-12-15T20:29:02Z

Ciaran Condon

2008-12-15T20:28:21Z

David Bechhofer

2008-12-15T20:28:00Z

Harald Putzer

2008-12-15T20:27:25Z

Richard Brennan

2008-12-15T20:27:00Z

Wolfgang Hillen

2008-12-15T20:26:36Z

Uwe Völker

2008-12-15T20:25:42Z

Michael Hecker

2008-12-15T20:25:29Z

Boris Görke

2008-12-15T20:25:10Z

Josef Deutscher

2008-12-15T20:24:27Z

88.70.2.125: /* Publications */

Josef Deutscher

2008-12-15T20:24:06Z

88.70.2.125: /* Lab head: Josef Deutscher */

= Lab head: Josef Deutscher=
'''Additional information'''
* [mailto:Josef.Deutscher@grignon.inra.fr send mail]
* location: Paris-Grignon, France
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Deutscher%2BJ%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests = sugar transport, phosphotransferase system (PTS), protein phosphorylation

= Open positions =

Josef Deutscher

2008-12-15T20:23:52Z

88.70.2.125: New page: = Lab head: Josef Deutscher= '''Additional information''' * [mailto:Josef.Deutscher@grignon.inra.fr send mail] * location: Pris-Grignon, France * Homepage: not available = Publications =...

= Lab head: Josef Deutscher=
'''Additional information'''
* [mailto:Josef.Deutscher@grignon.inra.fr send mail]
* location: Pris-Grignon, France
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Deutscher%2BJ%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests = sugar transport, phosphotransferase system (PTS), protein phosphorylation

= Open positions =

GapA

2008-12-15T20:17:38Z

88.70.2.125: /* References */

* '''Description:''' Glyceraldehyde 3-phosphate dehydrogenase, NAD-dependent, glycolytic enzyme 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gapA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || Yes [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 (PubMed)]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || glyceraldehyde 3-phosphate dehydrogenase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || catabolic enzyme in glycolysis
|-
|style="background:#ABCDEF;" align="center"| '''pI, MW''' || 5.033, 35676.2 Da
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1005 bp, 335 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cggR]]'', ''[[pgk]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gapA_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gapA_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:gapA_context.gif]]
|-
|}

__TOC__

 

=The gene=

== Basic information ==

* '''Coordinates:'''

==Phenotypes of a mutant ==

essential

== Database entries ==

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]

* '''SubtiList entry:'''[http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10827]

== Additional information==

=The protein=

== Basic information/ Evolution ==

* '''Catalyzed reaction/ biological activity:''' glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent). Catalyzes the reaction from glyceraldehyde-3-phosphate to 1.3-bi-phosphoglycerate. This reaction is part of the glycolysis.

* '''Protein family:'''

* '''Paralogous protein(s):''' [[GapB]]

== Extended information on the protein ==

* '''Kinetic information:''' K(M) for NAD: 5.7 mM, K(cat) for NAD: 70/sec (determined for GapA from ''Geobacillus stearothermophilus'') [http://www.ncbi.nlm.nih.gov/sites/entrez/10799476 PubMed]

* '''Domains:'''

* '''Modification:''' Phosphorylation (STY)

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''
** GapA-[[PtsH]]: [[PtsH|HPr(Ser-46-P)]] binds GapA resulting in a slight inhibition of enzymatic activity.[http://www.ncbi.nlm.nih.gov/sites/entrez/17142398 PubMed]
** GapA-[[Crh]]: [[Crh|Crh(Ser-46-P)]] binds GapA resulting in a slight inhibition of enzymatic activity.[http://www.ncbi.nlm.nih.gov/sites/entrez/17142398 PubMed]

* '''Localization:''' membrane and cytoplasm

== Database entries ==

* '''Structure:'''
** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3CMC 3CMC] (from ''Geobacillus stearothermophilus'')
** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NQO 1NQO] (from ''Geobacillus stearothermophilus'', mutant with cys 149 replaced by ser, complex with NAD+ und D-Glyceraldehyde-3-Phosphate)

* '''Swiss prot entry:''' [http://www.expasy.ch/cgi-bin/sprot-search-ac?P09124 P09124]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33940 KEGG]

* '''E.C. number:''' [http://www.expasy.ch/cgi-bin/get-enzyme-entry?1.2.1.12 1.2.1.12]

== Additional information==

GAP dehydrogenases from different sources (incl. ''Geobacillus stearothermophilus'') were shown to cleave RNA ([http://www.ncbi.nlm.nih.gov/sites/entrez/12359717 PubMed]). Moreover, mutations in ''gapA'' from ''B. subtilis'' can suppress mutations in genes involved in DNA replication ([http://www.ncbi.nlm.nih.gov/sites/entrez/17505547 PubMed]).

=Expression and regulation=

* '''Operon:'''
** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]''
** ''[[cggR]]-[[gapA]]''

The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the ''[[cggR]]'' open reading frame. This results in stable mature ''[[gapA]]'' and ''[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' mRNAs. The processing event requires the [[Rny]] protein.

* '''Sigma factor:''' SigA

* '''Regulation:''' [[CggR]] represses the operon in the absence of glycolytic sugars

* '''Regulatory mechanism:''' repression

* '''Database entries:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html DBTBS]

* '''Additional information:''' GapA is one of the most abundant proteins in the cell. In the presence of glucose, there are about 25,000 GapA molecules per cell ([http://www.ncbi.nlm.nih.gov/sites/entrez/12634343 PubMed]).

=Biological materials =

* '''Mutant:'''

* '''Expression vector:''' pGP90 (N-terminal Strep-tag, purification from ''B. subtilis''), pGP704 (N-terminal His-tag) (available in Stülke lab)

* '''lacZ fusion:''' pGP506 (in pAC7), pGP512 (in pAC6) (available in Stülke lab)

* '''GFP fusion:'''

* '''Antibody:''' available in Stülke lab

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html]

=Your additional remarks=

=References=

# Evguenieva-Hackenberg, E., Schiltz, E., and Klug, G. (2002) Dehydrogenases from all three domains of life cleave RNA. J Biol Chem 277, 46145-46150. [http://www.ncbi.nlm.nih.gov/sites/entrez/12359717 PubMed]
# Fillinger, S., Boschi-Muller, S., Azza, S., Dervyn, E., Branlant, G., and Aymerich, S. (2000) Two glyceraldehyde-3-phosphate dehydrogenases with opposite physiological roles in a nonphotosynthetic bacterium. J Biol Chem 275, 14031-14037. [http://www.ncbi.nlm.nih.gov/sites/entrez/10799476 PubMed]
# Jannière, L., Canceill, D., Suski, C., Kanga, S., Dalmais, B., Lestini, R., Monnier, A. F., Chapuis, J., Bolotin, A., Titok, M., Le Chatelier, E., and Ehrlich, S. D. (2007) Genetic evidence for a link between glycolysis and DNA replication. PLoS ONE 2, e447. [http://www.ncbi.nlm.nih.gov/sites/entrez/17505547 PubMed]
# Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in ''Bacillus subtilis'': evidence for the presence of multiple levels of control of the ''gapA'' operon. Mol Microbiol 41, 409-422.[http://www.ncbi.nlm.nih.gov/sites/entrez/11489127 PubMed]
# Ludwig, H., Rebhan, N., Blencke, H.-M., Merzbacher, M. & Stülke, J. (2002). Control of the glycolytic ''gapA'' operon by the catabolite control protein A in ''Bacillus subtilis'': a novel mechanism of CcpA-mediated regulation. Mol Microbiol 45, 543-553.[http://www.ncbi.nlm.nih.gov/sites/entrez/12123463 PubMed]
# Meinken, C., Blencke, H. M., Ludwig, H., and Stülke, J. (2003) Expression of the glycolytic ''gapA'' operon in ''Bacillus subtilis'': differential synthesis of proteins encoded by the operon. Microbiology 149, 751-761. [http://www.ncbi.nlm.nih.gov/sites/entrez/12634343 PubMed]
# Pompeo ''et al.'' (2007) Interaction of GapA with HPr and its homologue, Crh: Novel levels of regulation of a key step of glycolysis in ''Bacillus subtilis''? J Bacteriol 189, 1154-1157.[http://www.ncbi.nlm.nih.gov/sites/entrez/17142398 PubMed]
# Thomaides, H. B., Davison, E. J., Burston, L., Johnson, H., Brown, D. R., Hunt, A. C., Errington, J., and Czaplewski, L. (2007) Essential bacterial functions encoded by gene pairs. J Bacteriol 189, 591-602. [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]
# Tobisch, S., Zühlke, D., Bernhardt, J., Stülke, J., and Hecker, M. (1999) Role of CcpA in regulation of the central pathways of carbon catabolism in ''Bacillus subtilis''. J Bacteriol 181, 6996-7004.[http://www.ncbi.nlm.nih.gov/sites/entrez/10559165 PubMed]

GltC

2008-12-15T20:15:27Z

88.70.2.125: /* References */

* '''Description:''' Transcriptional activator of the ''gltAB'' operon. Activates expression of the operon in the absence of arginine. 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gltC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || No
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator (LysR family)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || positive regulation of the glutamate synthase operon (''gltAB'')
|-
|style="background:#ABCDEF;" align="center"| '''pI, MW''' || 5.618, 33866.6 Da
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 900 bp, 300 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gltA]]'', ''[[proJ]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gltC_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gltC_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:gltC_context.gif]]
|-
|}

__TOC__

 

=The gene=

== Basic information ==

* '''Coordinates:'''

==Phenotypes of a mutant ==

== Database entries ==

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltC.html]

* '''SubtiList entry:'''[http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10810]

== Additional information==

=The protein=

== Basic information/ Evolution ==

* '''Catalyzed reaction/ biological activity:''' transcription activation of the ''gltAB'' operon [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed]

* '''Protein family:''' LysR-type transcription regulator [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed]

* '''Paralogous protein(s):''' none, but there are 19 members of the LysR family in ''B. subtilis''

== Extended information on the protein ==

* '''Kinetic information:'''

* '''Domains:''' DNA-binding helix-turn-helix motif: AA 18 ... 37

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/17134717 PubMed]

* '''Interactions:'''
** GltC-[[RocG]], This interaction takes place in the presence of glutamate. It prevents the transcription activation of the ''gltAB'' operon. Note that [[RocG]] expression is strongly regulated. [http://www.ncbi.nlm.nih.gov/sites/entrez/17608797 PubMed]

* '''Localization:'''

== Database entries ==

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.expasy.ch/cgi-bin/sprot-search-ac?P20668 P20668]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18460 KEGG]

== Additional information==

=Expression and regulation=

* '''Operon:''' ''gltC''

* '''Sigma factor:''' SigA

* '''Regulation:''' autoregulation by GltC

* '''Regulatory mechanism:''' autorepression

* '''Database entries:''' [http://dbtbs.hgc.jp/COG/prom/gltC.html DBTBS]

* ''' Additional information:'''

=Biological materials =

* '''Mutant:''' GP344 (erm), GP738 (spc) (available in Stülke lab)

* '''Expression vector:''' pGP903 (N-terminal His-tag) (available in Stülke lab)

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''Antibody:''' available in Stülke lab

=Labs working on this gene/protein=

[[Linc Sonenshein|Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=

# Belitsky, B. R., and Sonenshein, A. L. (1995) Mutations in GltC that increase ''Bacillus subtilis gltA'' expression. J Bacteriol 177: 5696-5700.[http://www.ncbi.nlm.nih.gov/sites/entrez/7559360 PubMed]
# Belitsky, B. R., and Sonenshein, A. L. (2004) Modulation of activity of ''Bacillus subtilis'' regulatory proteins GltC and TnrA by glutamate dehydrogenase. J Bacteriol 186: 3399-3407.[http://www.ncbi.nlm.nih.gov/sites/entrez/15150225 PubMed]
# Bohannon, D. E., and Sonenshein, A. L. (1989) Positive regulation of glutamate biosynthesis in ''Bacillus subtilis''. J Bacteriol 171: 4718-4727.[http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed]
# Commichau, F. M., Wacker, I., Schleider, J., Blencke, H.-M., Reif, I., Tripal, P., and Stülke, J. (2007) Characterization of ''Bacillus subtilis'' mutants with carbon source-independent glutamate biosynthesis. J Mol Microbiol Biotechnol 12: 106-113. [http://www.ncbi.nlm.nih.gov/sites/entrez/17183217 PubMed]
# Commichau, F. M., Herzberg, C., Tripal, P., Valerius, O., and Stülke, J. (2007) A regulatory protein-protein interaction governs glutamate biosynthesis in ''Bacillus subtilis'': The glutamate dehydrogenase RocG moonlights in controlling the transcription factor GltC. Mol Microbiol 65: 642-654. [http://www.ncbi.nlm.nih.gov/sites/entrez/17608797 PubMed]
# Picossi, S., Belitsky, B. R., and Sonenshein, A. L. (2007) Molecular mechanism of the regulation of ''Bacillus subtilis gltAB'' expression by GltC. J Mol Biol 365: 1298-1313. [http://www.ncbi.nlm.nih.gov/sites/entrez/17134717 PubMed]
# Wacker, I., Ludwig, H., Reif, I., Blencke, H. M., Detsch, C., and Stülke, J. (2003) The regulatory link between carbon and nitrogen metabolism in ''Bacillus subtilis'': regulation of the ''gltAB'' operon by the catabolite control protein CcpA. Microbiology 149: 3001-3009.[http://www.ncbi.nlm.nih.gov/sites/entrez/14523131 PubMed]