cymR
168
pleiotropic regulator of sulfur metabolism
locus
BSU_27520
Molecular weight
11.75 kDa
pI
9.88
function
regulation of sulfur metabolism
product
transcription repressor
essential
no
synonyms
cymR, yrzC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1959 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,811,717 2,812,133
Phenotypes of a mutant
impaired growth in the presence of cystine and increased sensitivity to hydrogen peroxide-, disulfide-, paraquat- and tellurite-induced stresses [Pubmed|20608979]
increased intracellular cysteine pool and hydrogen sulfide formation [Pubmed|20608979]
depletion of branched-chain amino acids [Pubmed|20608979]
The protein
Protein family
Rrf2 family of transcription regulators
[wiki|Domains]
[wiki|HTH rrf2-type domain] (aa 2-125) (according to UniProt)
[wiki|Cofactors]
[protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK] acts as corepressor [Pubmed|18974048]
Structure
[PDB|2Y75] [Pubmed|21624051]
[AF|O34527]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
description
[Pubmed|22383849]
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
description
[Pubmed|16885442]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16885442], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B515 (yrzC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1514 NBRP B. subtilis, Japan]
BKE27520 ([gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATGAACACCTCTGTTA, downstream forward: _UP4_ATTTAGATCAGAGGTGTAAA
BKK27520 ([gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATGAACACCTCTGTTA, downstream forward: _UP4_ATTTAGATCAGAGGTGTAAA
labs
[wiki|Isabelle Martin-Verstraete], Institute Pasteur, Paris, France
References
Other original publications
The [wiki|CymR regulon]
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Time of last update: 2024-11-18 16:37:06
Author of last update: Jstuelk