cshB
168
DEAD-box RNA helicase, important for adaptation to low temperatures
locus
BSU_25140
Molecular weight
49.86 kDa
pI
9.89
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
cshB, yqfR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,594,184 → 2,595,500
Phenotypes of a mutant
poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]
The protein
Catalyzed reaction/ biological activity
RNA helicase
required for [wiki|ribosome] assembly [Pubmed|23175651]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|DEAD-box RNA helicases] (according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] (aa 35-208) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 235-385) (according to UniProt)
Structure
[PDB|5IVL] ([protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA], 80% coverage, 40% identity) [Pubmed|28238534]
[AF|P54475]
Paralogous protein(s)
[protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA], [protein|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD], [protein|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]
[wiki|Localization]
cytoplasma, colocalizes with the ribosomes [Pubmed|16352840]
Expression and Regulation
Operons
genes
[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C489 ([gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2487 NBRP B. subtilis, Japan]
GP1051 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::cat), available in [wiki|Jörg Stülke]'s lab
BKE25140 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA, downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA
BKK25140 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA, downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], expression from the native chromomsomal site: GP1064 (spc), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|20572937]
FLAG-tag construct
GP1011 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
References
Reviews
Original Publications
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Time of last update: 2024-11-18 00:56:16
Author of last update: Jstuelk