gpsB
168
cell cycle regulator, cytosolic adaptor for multiple cell wall enzymes, required for removal of [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1] from the cell pole after completion of cell pole maturation
locus
BSU_22180
Molecular weight
11.45 kDa
pI
5.63
function
[wiki|cell division], cell elongation, spatiotemporal control of penicillin‐binding protein activity
product
[protein|search|cell division] adaptor protein, member of the [wiki|divisome]
essential
no
synonyms
gpsB, ypsB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3599 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,331,779 → 2,332,075
Phenotypes of a mutant
synthetic lethal phenotype with a ''[gene|672EA84D7725BE21F649DF30A11EB4E0EDFC3925|ftsA]'' mutation [Pubmed|18776011]; synthetic sick phenotype when combined with an ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' mutation [Pubmed|18363795], although this seems controversial [Pubmed|18776011]
reduced growth at high salt [Pubmed|25845974]
The protein
Catalyzed reaction/ biological activity
activates [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] [Pubmed|25845974]
Protein family
GpsB family (single member, according to UniProt)
[wiki|Domains]
contains a [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|divIVA]-like N-terminal lipid binding domain [pubmed|30887576]
Structure
[PDB|4UG3] (N-terminal domain, aa 1-68) [Pubmed|26575090]
[PDB|5AN5] (C-terminal domain, aa 76-98) [Pubmed|26575090]
GpsB is hexameric (trimer of dimers) [Pubmed|26575090]
[PDB|6GP7] (Complexed with peptide fragment of PBP1A) [Pubmed|30651563]
[PDB|6GPZ] (Complexed with peptide fragment of L. monocytogenes PBP1A) [Pubmed|30651563]
[AF|P0CI74]
Modification
phosphorylation on Thr-75 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] [Pubmed|25845974,17218307]
Paralogous protein(s)
[protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|divIVA]
[wiki|Localization]
dynamic, shuttles between lateral membrane and the divison septum [Pubmed|18363795], localizes to the divisome after Z-ring formation [Pubmed|18776011]
Expression and Regulation
Operons
genes
[gene|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB]-[gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[gene|0DF27BEAF763545258CE7DD56F65CD5BF74DD887|ypsC]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A472 (gpsB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/472 NBRP B. subtilis, Japan]
1A934 ( ''gpsB''::''kan''), [Pubmed|18776011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
1A970 ( ''gpsB''::''kan''), [Pubmed|18776011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A970&Search=1A970 BGSC]
BKE22180 (Δ[gene|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAAT
BKK22180 (Δ[gene|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAAT
two-hybrid system
[Pubmed|18363795]
Antibody
raised against L. monocytogenes GpsB [Pubmed|26575090] available in [wiki|Sven Halbedel] 's lab
GFP fusion
[Pubmed|18363795,18776011]
References
Reviews
Original Publications
Page visits: 5122
Time of last update: 2024-11-05 05:29:29
Author of last update: Jstuelk