ureB
168
urease (beta subunit)
locus
BSU_36650
Molecular weight
13.50 kDa
pI
9.75
function
utilization of urea as alternative nitrogen source
product
urease (beta subunit)
essential
no
ec
3.5.1.5
synonyms
ureB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0832 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,768,420 → 3,768,794
The protein
Catalyzed reaction/ biological activity
2 H+ + H2O + urea --> CO2 + 2 NH4+ (according to UniProt)
Protein family
urease beta subunit family (single member, according to UniProt)
Structure
[PDB|3UBP] (from B. pasteurii, 48% identity) [pubmed|10368287]
[AF|P71035]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|63CF8C8BBCE39BD585DD5F6C8BBD95E12B500AE6|ywzF]-[gene|1D9596609AEF10456041918BD592E49466D93B1F|ywzE]-[gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]
description
[Pubmed|22383849]
genes
[gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]
description
[Pubmed|9287005]
regulation
induced by nitrogen limitation ([protein|search|GlnR], [protein|search|TnrA]) [Pubmed|9287005]
regulatory mechanism
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, [Pubmed|9287005], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|9287005], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12374841], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|9287005,12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9287005], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|9287005], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Biological materials
Mutant
BKE36650 (Δ[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCAATTTGAAATGCTCCCG, downstream forward: _UP4_GGCTGGATGGAGGGTGTAAT
BKK36650 (Δ[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCAATTTGAAATGCTCCCG, downstream forward: _UP4_GGCTGGATGGAGGGTGTAAT
References
Reviews
Original Publications
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Time of last update: 2025-05-02 15:34:19
Author of last update: Melvin.boenninger