clpC
168
AAA unfoldase, ATPase subunit of the [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] protease, directs proteins phosphorylated on arginine residues to [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]
locus
BSU_00860
Molecular weight
89.93 kDa
pI
5.75
function
protein degradation, positive regulator of autolysin ([protein|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC] and [protein|29219315D31BA4ADE41687EFF17FE41D6C23D157|lytD]) synthesis
product
AAA unfoldase, ATPase subunit of the [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] protease
essential
no
synonyms
clpC, mecB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0542 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
103,572 106,004
Phenotypes of a mutant
inactivation of [gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] reduces sporulation efficiency to 0.4% that of wild type cells; delayed entry into sporulation, defect in engulfment with reduced SigG activity, and production of small spores [Pubmed|26735940]
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
ATPase/chaperone
Protein family
ClpA/ClpB family (with [protein|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE], according to UniProt)
[wiki|Domains]
AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
[wiki|UVR domain] (aa 417-452) (according to UniProt)
Structure
[PDB|2K77] (N-terminal domain)
[PDB|3PXG] [pubmed|21368759]
[PDB|3J3U] (the [protein|331993A875907C10C77105FD8DDD86D4412CE405|mecA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] complex) [Pubmed|23595989]
[PDB|8B3S] (the [protein|7CFDFB42AF816FF5766086E537472008E6CB7E85|yjbA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] complex) [https://www.biorxiv.org/content/10.1101/2024.03.02.583065v1.full.pdf bioRxiv]
[AF|P37571]
Modification
phosphorylated on Arg-5 and Arg-254 [Pubmed|22517742]
phosphorylated on Arg-70 [Pubmed|31221751]
Paralogous protein(s)
[protein|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE]
[wiki|Localization]
cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] [http://www.ncbi.nlm.nih.gov/pubmed/18786145 Pubmed]
forms foci coincident with nucleoid edges, usually near cell poles [Pubmed|18689473]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
Expression and Regulation
Operons
genes
[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
description
[Pubmed|8793870]
regulation
expressed during germination and spore outgrowth [Pubmed|24244006]
regulatory mechanism
[protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|protein:908DB17A39D518E84977250C55825E77FA02E391|ctsR regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|30962353], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8793870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
GP3782 (Δ[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]::neo) available in [wiki|Jörg Stülke]'s lab
[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
BP98 (''clpC''::''spc''), available in [wiki|Fabian Commichau]'s lab [Pubmed|25610436]
BKE00860 ([gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCGTTCTGTAAATCTTCCAA, downstream forward: _UP4_TAATATAGAAGACGGAAATG
BKK00860 ([gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCGTTCTGTAAATCTTCCAA, downstream forward: _UP4_TAATATAGAAGACGGAAATG
Expression vectors
pGP3684 (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Ulf Gerth]'s and [wiki|Jörg Stülke]'s labs
GFP fusion
C-terminal GFP fusions (single copy, also as CFP and YFP variants) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
labs
[wiki|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
[wiki|Kürsad Turgay], Freie Universitt Berlin, Germany [http://www.biologie.fu-berlin.de/en/microbio/mibi-turg/index.html homepage]
References
Reviews
Original Publications
Page visits: 13229
Time of last update: 2024-11-17 20:27:32
Author of last update: Jstuelk