Lip

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  • Description: extracellular lipase

Gene name lip
Synonyms lipA
Essential no
Product extracellular lipase
Function lipid degradation
Gene expression levels in SubtiExpress: lip
MW, pI 22 kDa, 10.059
Gene length, protein length 636 bp, 212 aa
Immediate neighbours ansZ, yczC
Sequences Protein DNA DNA_with_flanks
Genetic context
Lip context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Lip expression.png















Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU02700

Expression

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): LipB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Bipin Singh, Gopalakrishnan Bulusu, Abhijit Mitra
Understanding the thermostability and activity of Bacillus subtilis lipase mutants: insights from molecular dynamics simulations.
J Phys Chem B: 2015, 119(2);392-409
[PubMed:25495458] [WorldCat.org] [DOI] (I p)

Ashutosh Srivastava, Somdatta Sinha
Thermostability of in vitro evolved Bacillus subtilis lipase A: a network and dynamics perspective.
PLoS One: 2014, 9(8);e102856
[PubMed:25122499] [WorldCat.org] [DOI] (I e)

Feifei Tian, Cao Yang, Congcong Wang, Tailin Guo, Peng Zhou
Mutatomics analysis of the systematic thermostability profile of Bacillus subtilis lipase A.
J Mol Model: 2014, 20(6);2257
[PubMed:24827611] [WorldCat.org] [DOI] (I p)

Virender Kumar, Poornima Yedavalli, Vishal Gupta, Nalam Madhusudhana Rao
Engineering lipase A from mesophilic Bacillus subtilis for activity at low temperatures.
Protein Eng Des Sel: 2014, 27(3);73-82
[PubMed:24402332] [WorldCat.org] [DOI] (I p)

Md Zahid Kamal, Jamshaid Ali, Nalam Madhusudhana Rao
Binding of bis-ANS to Bacillus subtilis lipase: a combined computational and experimental investigation.
Biochim Biophys Acta: 2013, 1834(8);1501-9
[PubMed:23639749] [WorldCat.org] [DOI] (P p)

Poornima Yedavalli, Nalam Madhusudhana Rao
Engineering the loops in a lipase for stability in DMSO.
Protein Eng Des Sel: 2013, 26(4);317-24
[PubMed:23404771] [WorldCat.org] [DOI] (I p)

Wojciech Augustyniak, Hans Wienk, Rolf Boelens, Manfred T Reetz
¹H, ¹³C and ¹⁵N resonance assignments of wild-type Bacillus subtilis Lipase A and its mutant evolved towards thermostability.
Biomol NMR Assign: 2013, 7(2);249-52
[PubMed:22996591] [WorldCat.org] [DOI] (I p)

Wojciech Augustyniak, Agnieszka A Brzezinska, Tjaard Pijning, Hans Wienk, Rolf Boelens, Bauke W Dijkstra, Manfred T Reetz
Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention.
Protein Sci: 2012, 21(4);487-97
[PubMed:22267088] [WorldCat.org] [DOI] (I p)

Shoeb Ahmad, Virender Kumar, K Bhanu Ramanand, N Madhusudhana Rao
Probing protein stability and proteolytic resistance by loop scanning: a comprehensive mutational analysis.
Protein Sci: 2012, 21(3);433-46
[PubMed:22246996] [WorldCat.org] [DOI] (I p)

Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947] [WorldCat.org] [DOI] (I p)

Zhong Ni, Peng Zhou, Xin Jin, Xian-Fu Lin
Integrating In Silico and In vitro approaches to dissect the stereoselectivity of Bacillus subtilis lipase A toward ketoprofen vinyl ester.
Chem Biol Drug Des: 2011, 78(2);301-8
[PubMed:21477088] [WorldCat.org] [DOI] (I p)

Bo Chen, Zhen Cai, Wei Wu, Yunlong Huang, Juergen Pleiss, Zhanglin Lin
Morphing activity between structurally similar enzymes: from heme-free bromoperoxidase to lipase.
Biochemistry: 2009, 48(48);11496-504
[PubMed:19883129] [WorldCat.org] [DOI] (I p)

Thijs R H M Kouwen, René van der Ploeg, Haike Antelmann, Michael Hecker, Georg Homuth, Ulrike Mäder, Jan Maarten van Dijl
Overflow of a hyper-produced secretory protein from the Bacillus Sec pathway into the Tat pathway for protein secretion as revealed by proteogenomics.
Proteomics: 2009, 9(4);1018-32
[PubMed:19180538] [WorldCat.org] [DOI] (I p)

Allison V Banse, Arnaud Chastanet, Lilah Rahn-Lee, Errett C Hobbs, Richard Losick
Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2008, 105(40);15547-52
[PubMed:18840696] [WorldCat.org] [DOI] (I p)

Ykelien L Boersma, Tjaard Pijning, Margriet S Bosma, Almer M van der Sloot, Luís F Godinho, Melloney J Dröge, Remko T Winter, Gertie van Pouderoyen, Bauke W Dijkstra, Wim J Quax
Loop grafting of Bacillus subtilis lipase A: inversion of enantioselectivity.
Chem Biol: 2008, 15(8);782-9
[PubMed:18721749] [WorldCat.org] [DOI] (P p)

Ykelien L Boersma, Melloney J Dröge, Almer M van der Sloot, Tjaard Pijning, Robbert H Cool, Bauke W Dijkstra, Wim J Quax
A novel genetic selection system for improved enantioselectivity of Bacillus subtilis lipase A.
Chembiochem: 2008, 9(7);1110-5
[PubMed:18383241] [WorldCat.org] [DOI] (I p)

Eerappa Rajakumara, Priyamvada Acharya, Shoeb Ahmad, Rajan Sankaranaryanan, Nalam M Rao
Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH.
Biochim Biophys Acta: 2008, 1784(2);302-11
[PubMed:18053819] [WorldCat.org] [DOI] (P p)

Melloney J Dröge, Ykelien L Boersma, Gertie van Pouderoyen, Titia E Vrenken, Carsten J Rüggeberg, Manfred T Reetz, Bauke W Dijkstra, Wim J Quax
Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection.
Chembiochem: 2006, 7(1);149-57
[PubMed:16342303] [WorldCat.org] [DOI] (P p)

Helga Westers, Peter G Braun, Lidia Westers, Haike Antelmann, Michael Hecker, Jan D H Jongbloed, Hirofumi Yoshikawa, Teruo Tanaka, Jan Maarten van Dijl, Wim J Quax
Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis.
Appl Environ Microbiol: 2005, 71(4);1899-908
[PubMed:15812018] [WorldCat.org] [DOI] (P p)

Thorsten Eggert, Ulf Brockmeier, Melloney J Dröge, Wim J Quax, Karl-Erich Jaeger
Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH.
FEMS Microbiol Lett: 2003, 225(2);319-24
[PubMed:12951259] [WorldCat.org] [DOI] (P p)

Melloney J Dröge, Carsten J Rüggeberg, Almer M van der Sloot, Judith Schimmel, Dolf Swaving Dijkstra, Raymond M D Verhaert, Manfred T Reetz, Wim J Quax
Binding of phage displayed Bacillus subtilis lipase A to a phosphonate suicide inhibitor.
J Biotechnol: 2003, 101(1);19-28
[PubMed:12523966] [WorldCat.org] [DOI] (P p)

Jan D H Jongbloed, Haike Antelmann, Michael Hecker, Reindert Nijland, Sierd Bron, Ulla Airaksinen, Frens Pries, Wim J Quax, Jan Maarten van Dijl, Peter G Braun
Selective contribution of the twin-arginine translocation pathway to protein secretion in Bacillus subtilis.
J Biol Chem: 2002, 277(46);44068-78
[PubMed:12218047] [WorldCat.org] [DOI] (P p)

Kosei Kawasaki, Hidemasa Kondo, Mamoru Suzuki, Satoru Ohgiya, Sakae Tsuda
Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 A resolution.
Acta Crystallogr D Biol Crystallogr: 2002, 58(Pt 7);1168-74
[PubMed:12077437] [WorldCat.org] [DOI] (P p)

T Eggert, G van Pouderoyen, B W Dijkstra, K E Jaeger
Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure.
FEBS Lett: 2001, 502(3);89-92
[PubMed:11583117] [WorldCat.org] [DOI] (P p)

G van Pouderoyen, T Eggert, K E Jaeger, B W Dijkstra
The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme.
J Mol Biol: 2001, 309(1);215-26
[PubMed:11491291] [WorldCat.org] [DOI] (P p)

E Lesuisse, K Schanck, C Colson
Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme.
Eur J Biochem: 1993, 216(1);155-60
[PubMed:8396026] [WorldCat.org] [DOI] (P p)

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