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• Description: DNA gyrase (subunit A)
  
  

Gene name	gyrA
Synonyms	nalA
Essential	yes PubMed
Product	DNA gyrase (subunit A)
Function	DNA supercoiling, initation of replication cycle and DNA elongation
Gene expression levels in SubtiExpress: GyrA
Interactions involving this protein in SubtInteract: GyrA
MW, pI	91 kDa, 5.215
Gene length, protein length	2463 bp, 821 aa
Immediate neighbours	gyrB, rrnO-16S
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

The gene

Basic information

• Locus tag: BSU00070

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)

• Protein family: topoisomerase gyrA/parC subunit family (according to Swiss-Prot)

• Paralogous protein(s): ParC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GyrA-GyrB

• Localization:
  • Nucleoid (Homogeneous) PubMed
  • nucleoid-associated in actively growing cells PubMed

Database entries

• Structure:
  • 4DDQ (DNA gyrase A-subunit lacking the C-terminal DNA-wrapping domains) PubMed

• UniProt: P05653

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: gyrA PubMed

• Expression browser: gyrA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed, GyrA is subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage